A5086671760- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Extracellular vesicles in disease
- Mass Spectrometry Techniques and Applications
- Natural product bioactivities and synthesis
- Metabolomics and Mass Spectrometry Studies
- Ginkgo biloba and Cashew Applications
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Boron Compounds in Chemistry
- Microfluidic and Capillary Electrophoresis Applications
- Phytochemicals and Antioxidant Activities
- Neurological Disease Mechanisms and Treatments
- Redox biology and oxidative stress
- Nuclear Physics and Applications
- Glycosylation and Glycoproteins Research
- Selenium in Biological Systems
- Bacteriophages and microbial interactions
- Bioinformatics and Genomic Networks
- Galectins and Cancer Biology
- Bacterial Genetics and Biotechnology
- Alzheimer's disease research and treatments
- Radiopharmaceutical Chemistry and Applications
- Enzyme Structure and Function
- MicroRNA in disease regulation
- T-cell and B-cell Immunology
Institute of Biomedical Technologies
2016-2025
National Research Council
2016-2025
National Academies of Sciences, Engineering, and Medicine
2010-2025
Istituto per le Tecnologie Didattiche
2006-2025
Institute for Experimental Endocrinology and Oncology
2025
Institute of Experimental Endocrinology of the Slovak Academy of Sciences
2025
Scuola Superiore Sant'Anna
2013-2024
CIMA Research Foundation
2022
Institute of Genetic and Biomedical Research
2022
Deutsche Gesellschaft für Muskelkranke
2021
Abstract Background: The national programs for the harmonization of hemoglobin (Hb)A1c measurements in US [National Glycohemoglobin Standardization Program (NGSP)], Japan [Japanese Diabetes Society (JDS)/Japanese Clinical Chemistry (JSCC)], and Sweden are based on different designated comparison methods (DCMs). future basis international standardization will be reference system developed by IFCC Working Group HbA1c Standardization. aim present study was to determine relationships between...
HbA 1c is the stable glucose adduct to N-terminal group of β-chain 0 . The measurement in human blood most important for long-term control glycaemic state diabetic patients. Because there was no internationally agreed reference method IFCC Working Group on Standardization developed a which here described. In first step haemoglobin cleaved into peptides by enzyme endoproteinase Glu-C, and second glycated non-glycated hexapeptides obtained are separated quantified HPLC electrospray ionisation...
Tumor-promoted constraints negatively affect cytotoxic T lymphocyte (CTL) trafficking to the tumor core and, as a result, inhibit killing. The production of reactive nitrogen species (RNS) within microenvironment has been reported in mouse and human cancers. We describe novel RNS-dependent posttranslational modification chemokines that profound impact on leukocyte recruitment tumors. Intratumoral RNS induces CCL2 chemokine nitration hinders cell infiltration, resulting trapping...
Human CD4(+)CD25(hi)Foxp3(+)CD127(-) Treg and CD4(+)CD25(-)Foxp3(-) Tconv cell functions are governed by their metabolic requirements. Here we report a comprehensive comparative analysis between ex vivo human cells that comprises analyses of the proteomic networks in subcellular compartments. We identified dominant signature at level primarily impacted highly-tuned balance glucose fatty-acid oxidation two types. Ex were highly glycolytic while used predominantly (FAO). When cultured vitro,...
Cell therapy trials using cardiac-resident progenitor cells (CPCs) and bone marrow-derived mesenchymal stem/progenitor (BMCs) in patients after myocardial infarction have provided encouraging results. Exosomes, nanosized extracellular vesicles of endosomal origin, figure prominently the bioactivities these cells. However, a head-to-head comparison exosomes from two cell types has not been performed yet. CPCs BMCs were derived cardiac atrial appendage specimens sternal marrow, respectively,...
Commonly used medicinal plant extracts with standardized content of polyphenols were investigated for their total antioxidant activity (TAA). Green tea, oligomeric procyanidins (from grape seed and pine bark), bilberry, ginkgo exhibited TAA in the range 5.12−2.57 mM Trolox, thereby indicating a valuable capacity. Witch hazel, propolis EPID, artichoke, hawthorn afforded lower (1.54−0.44 Trolox), whereas echinacea, ginseng, passionflower, sweet clover, eleuthero rather uneffective (TAA < 0.32...
In GPxs, the redox-active Se or S, is at hydrogen bonding distance from Gln and Trp residues that contribute to catalysis. From sequence homology of >400 sequences modeling DmGPx as a paradigm, Asn136 emerged fourth essential component active site. Mutational substitution by His, Ala, Asp results in dramatic decline specific activity. Kinetic analysis indicates k+1, rate constant for oxidation enzyme, decreases two three orders magnitude, whereas reductive steps characterized k′+2 are less...
Neuroblastoma (NB) is the most common extracranial solid tumor in childhood, with grim prognosis a half of patients. Exosomes are nanometer-sized membrane vesicles derived from multivesicular bodies (MVBs) endocytic pathway and released by normal neoplastic cells. Tumor-derived exosomes have been shown different model systems to carry molecules that promote cancer growth dissemination. In this respect, we here performed first characterization proteomic analysis isolated human NB cell lines...
Abstract Systemic light chain amyloidosis (AL) is a life-threatening disease caused by aggregation and deposition of monoclonal immunoglobulin chains (LC) in target organs. Severity heart involvement the most important factor determining prognosis. Here, we report 4.0 Å resolution cryo-electron microscopy map molecular model amyloid fibrils extracted from an AL patient with severe cardiomyopathy. The helical are composed single protofilament, showing typical 4.9 stacking cross-β...
In this paper, a pilot production process for mesenchymal stem/stromal freeze-dried secretome was performed in validated good manufacturing practice (GMP)-compliant cell factory. Secretome purified from culture supernatants by ultrafiltration, added to cryoprotectant, lyophilized and characterized. We obtained freeze-dried, "ready-off-the-shelf" free soluble powder containing extracellular vesicles proteins. the product, not-aggregated population of detected nanoparticle tracking analysis;...
Combined administration of anthracyclines (e.g. doxorubicin; Dox) and trastuzumab (Trz), a humanized anti-human epidermal growth factor receptor 2 (HER2; ErbB2), is an effective treatment for HER2-positive breast cancer. However, both agents are associated with cardiac toxicity. Human cardiac-resident mesenchymal progenitor cells (CPCs) secrete extracellular vesicles including nanosized exosomes which protect against myocardial ischaemia. Here, we investigated the effects these using novel...
Abstract During aging, neuronal organelles filled with neuromelanin (a dark-brown pigment) and lipid bodies accumulate in the brain, particularly substantia nigra, a region targeted Parkinson’s disease. We have investigated protein systems involved formation of these synthesis human nigra. Membrane matrix proteins characteristic lysosomes were found neuromelanin-containing at lower number than typical lysosomes, indicating reduced enzymatic activity likely impaired capacity for lysosomal...
Asthma is a chronic inflammatory disease.Reticular basement membrane (RBM) thickening considered feature of airway remodelling (AR) particularly in severe asthma (SA).Omalizumab, mAb to IgE effective SA and can modulate AR.Herein we describe protein profiles bronchial biopsies detect biomarkers anti-IgE effects on AR explain potential mechanisms/pathways.We defined the biopsy profiles, before after treatment.Unsupervised clustering baseline proteomes resulted very good agreement with...
Breast cancer is the most common among women, and metabolic syndrome (MetS) a risk factor for breast cancer, especially postmenopausal cancer. We evaluated role of advanced glycated end products (AGEs) levels contributing to association between MetS risk. Plasma AGEs were measured in case-control study nested within Hormones Diet Etiology Tumors (ORDET) cohort, including 40 incident cases (20 with 20 without) controls without). The was analyzed using Bayesian logistic regression models. An...
Rooibos tea originates from the leaves and stems of indigenous South African plant Aspalathus linearis. It has gained much attention for clinical purposes in case nervous tension, allergies (dermatitis), various indigestive problems. Recently, antioxidative activity was also attributed to on basis its flavonoid content. Therefore, an HPLC method using a C(18) reversed phase column developed assay 10 flavonoids aqueous methanolic infusions. Main compounds determined were dihydrochalcone...
The purpose of this study is to identify novel proteins released by cancer cells that are involved in extracellular matrix (ECM) remodeling using small-volume samples and automated technology. We applied multidimensional protein identification technology (MudPIT), which incorporates two-dimensional capillary chromatography coupled tandem mass spectrometry small quantities serum-free supernatants resting or phorbol ester-activated Suit-2 pancreatic cells. Selected markers were validated...