A5020221014- Silk-based biomaterials and applications
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Antimicrobial Peptides and Activities
- Extracellular vesicles in disease
- Tissue Engineering and Regenerative Medicine
- Biochemical and Structural Characterization
- Periodontal Regeneration and Treatments
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Osteoarthritis Treatment and Mechanisms
- Wound Healing and Treatments
- Phytochemical compounds biological activities
- Protein Hydrolysis and Bioactive Peptides
- Protease and Inhibitor Mechanisms
- RNA Interference and Gene Delivery
- Graphene and Nanomaterials Applications
- Bee Products Chemical Analysis
- Tendon Structure and Treatment
- Advancements in Transdermal Drug Delivery
- Saffron Plant Research Studies
- Microencapsulation and Drying Processes
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Production and Characterization
- Xenotransplantation and immune response
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2022-2024
Sapienza University of Rome
2024
University of Pavia
2017-2021
In this paper, a pilot production process for mesenchymal stem/stromal freeze-dried secretome was performed in validated good manufacturing practice (GMP)-compliant cell factory. Secretome purified from culture supernatants by ultrafiltration, added to cryoprotectant, lyophilized and characterized. We obtained freeze-dried, "ready-off-the-shelf" free soluble powder containing extracellular vesicles proteins. the product, not-aggregated population of detected nanoparticle tracking analysis;...
From the end of 2019, world population has been faced spread novel coronavirus SARS-CoV-2 responsible for COVID-19 infection. In approximately 14% patients affected by coronavirus, infection progresses with development pneumonia that requires mechanical ventilation. At moment, there is no specific antiviral treatment recommended pandemic and therapeutic strategies to deal are only supportive. our opinion, mesenchymal stem cell secretome could offer a new approach in treating pneumonia, due...
To validate the use of ultrafiltration (UF) as an alternative applicable industrial method to replace ultracentrifugation (UC) in purification mesenchymal stromal cell (MSC)-secretome.Pharmaceutical formulations containing secretome and/or extracellular vesicles were extracted from adipose-MSCs and bone marrow-MSCs by combining UF or UC with lyophilization.UF led higher protein, lipid, cytokine exosomes yields compared UC. The isolation procedure source influenced immunomodulatory activity,...
Sodium alginate (SA)-based hydrogels are often employed as bioink for three-dimensional (3D) scaffold bioprinting. They offer a suitable environment cell proliferation and differentiation during tissue regeneration also control the release of growth factors mesenchymal stem secretome, which is useful biointegration. However, such show poor mechanical properties, fast-release kinetics, low biological performance, hampering their successful clinical application. In this work, silk fibroin...
Accumulating evidence shows that Mesenchymal Stem/Stromal Cells (MSCs) exert their therapeutic effects by the release of secretome, made both soluble proteins and nano/microstructured extracellular vesicles (EVs). In this work, for first time, we proved a proteomic investigation adipose-derived (AD)-MSC-secretome contains alpha-1-antitrypsin (AAT), main elastase inhibitor in lung, 72 other involved protease/antiprotease balance, 46 response to bacteria. By secretome fractionation, AAT is...
Standard treatments of chronic skin ulcers based on the direct application dressings still present several limits with regard to a complete tissue regeneration. Innovative strategies in engineering offer materials that can tune cell behavior and promote growth favoring recruitment early stages wound healing. A combination Alginate (Alg), Sericin (SS) Platelet Lysate (PL), as freeze-dried sponge, is proposed generate bioactive dressing care lesions. Biomembranes at different composition were...
Three-dimensional printing of poly(ε-caprolactone) (PCL) is a consolidated scaffold manufacturing technique for bone regenerative medicine. Simultaneously, the mesenchymal stem/stromal cell (MSC) secretome osteoinductive, promoting colonization by cells, proliferation, and differentiation. The present paper combines 3D-printed PCL scaffolds with lyosecretome, freeze-dried formulation MSC secretome, containing proteins extracellular vesicles (EVs). We designed lyosecretome two loading...
Developing drug delivery systems to target cytotoxic drugs directly into tumor cells is still a compelling need with regard reducing side effects and improving the efficacy of cancer chemotherapy. In this work, silk fibroin nanoparticles (SFNs) have been designed load previously described compound (NDI-1) that disrupts cell cycle by specifically interacting non-canonical secondary structures DNA. SFNs were then functionalized on their surface cyclic pentapeptides incorporating Arg-Gly-Asp...
Some natural compounds have recently been widely employed in wound healing applications due to their biological properties. One such compound is sericin, which produced by Bombix mori, while active polyphenols, polysaccharides and proteins are synthetized Chlorella vulgaris Arthrospira platensis microalgae. Our hypothesis was that as an optimal bioactive polymeric carrier for microencapsulation process, could also improve the regenerative effect of A solvent-free extraction method spray...
Introduction: Initiation and progression of intervertebral disk degeneration are linked to oxidative stress, with reactive oxygen species being a key factor. Therefore, as potentially novel approach able regenerate the damaged disk, this work aimed prepare an “active per sé” drug delivery system by combining sericin crocetin: both bioactive compounds antioxidant, anti-inflammatory, immunomodulant regenerative properties. Methods: In detail, nanoparticles were prepared using crocetin...
Arg-Gly-Asp (RGD)-based cyclopentapeptides (cRGDs) have a high affinity towards integrin αvβ3 and αvβ5, which are overexpressed by many tumor cells. Here, curcumin-loaded silk fibroin nanoparticles (SFNs) been functionalized on the surface with cRGD to provide active targeting cells; “click reaction” between RGD-based cyclopentapeptide carrying an azide group triple-bond-functionalized has exploited. Both naked SFNs were less than 200 nm in diameter showed round-shaped morphology but, after...
To date, more than 100 million people worldwide have recovered from COVID-19. Unfortunately, although the virus is eradicated in such patients, fibrotic irreversible interstitial lung disease (pulmonary fibrosis, PF) clinically evident. Given vast numbers of individuals affected, it urgent to design a strategy prevent second wave late mortality associated with COVID-19 PF as long-term consequence devastating pandemic. Available antifibrotic therapies, namely nintedanib and pirfenidone, might...
The volatile fractions from fresh inflorescences of naturally growing orchids Anacamptis coriophora (L.) R. M. Bateman, Pridgeon & W. Chase subsp. fragrans (Pollini), pyramidalis Ophrys holosericea (Burm.) Greuter and Serapias vomeracea (Burm. f.) B. were isolated by steam distillation analyzed GC/FID GC/MS. Saturated hydrocarbons quantified as the major constituents fraction (47.87-81.57% total essential oil), which long-chain monounsaturated accounted 9.20% to 32.04% oil. Double bond...
Producing mesenchymal stem cell (MSC)-secretome for dose escalation studies and clinical practice requires scalable good manufacturing (GMP)-compliant production procedures formulation into a standardized medicinal product. Starting from method that combines ultrafiltration freeze-drying to transform MSC-secretome pharmaceutical product, the lyosecretome, this work aims to: (i) optimize lyosecretome formulation; (ii) investigate sources of variability can affect robustness process; (iii)...
Ionotropic gelation is a low-cost, easy and green microencapsulation technique. However, the encapsulation of highly soluble compounds challenging because wide loss material into external water phase by passive diffusion consequent low efficiency. In this work an important increase efficiency for Thymus vulgaris L. aqueous extract in alginate-based microparticles has been obtained. A formulation with proper thyme extract/alginate ratio (30:70) was used as reference then optimized adding...
Silk fibroin nanoparticles (SFNs) have been widely investigated for drug delivery, but their clinical application still faces technical (large-scale and GMP-compliant manufacturing), economic (cost-effectiveness in comparison to other polymer-based nanoparticles), biological (biodistribution assessments) challenges. To address biodistribution challenge, we provide a straightforward desolvation method (in acetone) produce homogeneous SFNs incorporating increasing amounts of Fe