A5036447979- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Dental Implant Techniques and Outcomes
- Orthopaedic implants and arthroplasty
- Wound Healing and Treatments
- Advanced X-ray Imaging Techniques
- Tissue Engineering and Regenerative Medicine
- Bone health and osteoporosis research
- Electrospun Nanofibers in Biomedical Applications
- Silk-based biomaterials and applications
- X-ray Diffraction in Crystallography
- 3D Printing in Biomedical Research
- Bone fractures and treatments
- Bone Metabolism and Diseases
- Traumatic Brain Injury and Neurovascular Disturbances
- Corneal Surgery and Treatments
- Osteoarthritis Treatment and Mechanisms
- Calcium Carbonate Crystallization and Inhibition
- Cell Adhesion Molecules Research
- Tendon Structure and Treatment
- Spine and Intervertebral Disc Pathology
- Anatomy and Medical Technology
- Extracellular vesicles in disease
- Bone and Dental Protein Studies
University of Genoa
2014-2024
Ospedale Policlinico San Martino
2012-2024
Alleanza Contro il Cancro
2006-2018
GTx (United States)
2017
National Cancer Research Institute
2015
Lomonosov Moscow State University
2011
Cornell University
2010
Tecnologie Avanzate (Italy)
2000-2006
New York Psychoanalytic Society and Institute
2004
New York State Psychiatric Institute
2004
Extensive bone loss is still a major problem in orthopedics. A number of different therapeutic approaches have been developed and proposed, but so far none proven to be fully satisfactory. We used new tissue engineering approach treat four patients with large diaphysis defects poor alternatives. To obtain implantable three-dimensional (3D) living constructs, cells isolated from the patients' marrow stroma were expanded culture seeded onto porous hydroxyapatite (HA) ceramic scaffolds designed...
Structured Abstract Authors – Mastrogiacomo M, Muraglia A, Komlev V, Peyrin F, Rustichelli Crovace Cancedda R Background Large bone defects still represent a major problem in orthopedics. Traditional bone‐repair treatments can be divided into two groups: the transport (Ilizarov technology) and graft transplant (autologous or allogeneic grafts). Thus far, none of these strategies have proven to always resolving. As an alternative, tissue engineering approach has been proposed where osteogenic...
Umbilical cord mesenchymal stem cells (UC-MSCs) show properties similar to bone marrow (BM-MSCs), although controversial data exist regarding their osteogenic potential. We prepared clinical-grade UC-MSCs from Wharton's Jelly and we investigated if could be used as substitutes for BM-MSCs in muscoloskeletal regeneration a more readily available functional source of MSCs. were loaded onto scaffolds implanted subcutaneously (ectopically) critical-sized calvarial defects (orthotopically) mice....
Bone-marrow stromal cells can differentiate into multiple mesenchymal lineages including cartilage and bone. When these are seeded in high-density 'pellet culture', they undergo chondrogenesis form a tissue that is morphologically biochemically defined as cartilage. Here, we show dual chondro-osteogenic differentiation be obtained the same micromass culture of human bone-marrow cells. Human were pellet cultured for 4 weeks chondro-inductive medium. Cartilage 'beads' resulting from then...
In this study we evaluated the performance of Skelite, a resorbable bioceramic based on silicon stabilized tricalcium phosphate (Si-TCP), in promoting repair large-sized, experimentally induced defect weight-bearing long bone sheep model. Eighteen 2-year-old ewes were used study. Animals sacrificed at 3, 6, and 12 months. One animal entered very prolonged followup was 2 years after surgery. Bone formation scaffold resorption by sequential x-ray studies, CT scans, histology, immunohistology,...
The heterogeneous population of non-haematopoietic cells residing in the bone marrow (bone stromal cells, BMSCs) and different fractions components obtained from platelet-rich plasma provide an invaluable source autologous growth factors for other connective tissue reconstruction. In this study, we investigated effect allogenic platelet lysate on human BMSCs proliferation differentiation. Cell number performed cell doublings were enhanced cultures supplemented with platelet-derived (platelet...
Human platelet lysate (hPL) is a pool of growth factors and cytokines able to induce regeneration different tissues. Despite its good potentiality as therapeutic tool for regenerative medicine applications, hPL has been only moderately exploited in this field. A more widespread adoption limited because rapid degradation at room temperature that decreases functionality. Another limiting factor extensive use the difficulty handling gels. In work, silk fibroin-based patches were developed...
Abstract Faults in vascular (VN) and neuronal networks of spinal cord are responsible for serious neurodegenerative pathologies. Because inadequate investigation tools, the lacking knowledge complete fine structure VN system represents a crucial problem. Conventional 2D imaging yields incomplete spatial coverage leading to possible data misinterpretation, whereas standard 3D computed tomography achieves insufficient resolution contrast. We show that X-ray high-resolution phase-contrast...
Abstract The degenerative effects of multiple sclerosis at the level vascular and neuronal networks in central nervous system are currently object intensive investigation. Preclinical studies have demonstrated efficacy mesenchymal stem cell (MSC) therapy experimental autoimmune encephalomyelitis (EAE), animal model for sclerosis, but neuropathology specific lesions EAE MSC treatment under debate. Because conventional imaging techniques entail protocols that alter tissues, limiting...
Tissue engineering approaches have recently been devised to repair large bone losses. takes advantages of the combined use cultured living cells and 3D scaffolds deliver vital damaged site patient. Cultured marrow stromal (BMSCs) can be regarded as a mesenchymal progenitor/precursor cell population derived from adult stem cells. When implanted in immunodeficient mice, BMSCs with mineralized form primary tissue that is highly vascularized. We used autologous BMSC/bioceramic composites treat...
Bone repair was addressed in a critical-sized defect model sheep, combining ceramic biomaterial and mesenchymal progenitor cells. The defects the tibial mid-diaphysis were treated with autologous bone or silicon-stabilized tricalcium phosphate biomaterial, implemented not by addition of expanded marrow stromal An internal locking compression plate an external fixator applied for stabilization. Radiographies taken during 8 months follow-up: pixel grey levels lesion areas determined to...
Platelet lysates (PL), which are derived from platelets, cocktails of growth factors and cytokines that can promote tissue regeneration. Until today, most studies have focused on factor content platelets rather than their potential as a reservoir mediators cytokines. Taking advantage an in vitro scratch assay performed under both normal inflammatory conditions, the present work, we report at physiologic concentrations, PL enhanced wound closure rates NCTC 2544 human keratinocytes. This...
The periosteum plays a pivotal role during bone development and repair contributing to vascularization osteoprogenitor cells source. We propose periosteal substitute engineered using platelet-rich plasma (PRP) membrane incorporating autologous marrow-derived mesenchymal stem (PRP/BMSC gel membrane) be wrapped around an osteoconductive scaffold for regeneration of compromised defects. PRP/BMSC was optimized different compositions optimal release vascular endothelial growth factor (VEGF)...
Present cell culture medium supplements, in most cases based on animal sera, are not fully satisfactory especially for the vitro expansion of cells intended human therapy. This paper refers to (i) an heparin-free platelet lysate (PL) devoid serum or plasma components (v-PL) and (ii) derived from PL (Pl-s) their use as single combination primary line cultures. Human mesenchymal stem (MSC) cultures were obtained adipose tissue, bone marrow, umbilical cord. chondrocytes articular cartilage...