A5024670718- Advanced X-ray Imaging Techniques
- Advanced Electron Microscopy Techniques and Applications
- X-ray Spectroscopy and Fluorescence Analysis
- Crystallography and Radiation Phenomena
- Particle Accelerators and Free-Electron Lasers
- Nuclear Physics and Applications
- Advanced MRI Techniques and Applications
- Medical Imaging Techniques and Applications
- Cultural Heritage Materials Analysis
- Advanced X-ray and CT Imaging
- Advanced Neuroimaging Techniques and Applications
- X-ray Diffraction in Crystallography
- Laser-Plasma Interactions and Diagnostics
- Surface Roughness and Optical Measurements
- Traumatic Brain Injury and Neurovascular Disturbances
- Bone Tissue Engineering Materials
- Photoacoustic and Ultrasonic Imaging
- Electron and X-Ray Spectroscopy Techniques
- Advancements in Photolithography Techniques
- Cell Image Analysis Techniques
- Ion-surface interactions and analysis
- Bone health and osteoporosis research
- Advanced Fluorescence Microscopy Techniques
- Image Processing and 3D Reconstruction
- Intracranial Aneurysms: Treatment and Complications
Istituto di Nanotecnologia
2016-2025
P.N. Lebedev Physical Institute of the Russian Academy of Sciences
2011-2024
Sapienza University of Rome
2013-2022
National Research Council
2006-2021
Istituto Nanoscienze
2018-2021
Istituto di Fotonica e Nanotecnologie
2005-2020
Institute of Nanotechnology
2020
Center for Biomolecular Nanotechnologies
2013-2014
Istituto Nazionale di Fisica Nucleare
2013
A.V. Shubnikov Institute of Crystallography
2011-2012
Abstract Faults in vascular (VN) and neuronal networks of spinal cord are responsible for serious neurodegenerative pathologies. Because inadequate investigation tools, the lacking knowledge complete fine structure VN system represents a crucial problem. Conventional 2D imaging yields incomplete spatial coverage leading to possible data misinterpretation, whereas standard 3D computed tomography achieves insufficient resolution contrast. We show that X-ray high-resolution phase-contrast...
Abstract The degenerative effects of multiple sclerosis at the level vascular and neuronal networks in central nervous system are currently object intensive investigation. Preclinical studies have demonstrated efficacy mesenchymal stem cell (MSC) therapy experimental autoimmune encephalomyelitis (EAE), animal model for sclerosis, but neuropathology specific lesions EAE MSC treatment under debate. Because conventional imaging techniques entail protocols that alter tissues, limiting...
Alzheimer’s disease (AD), a debilitating neurodegenerative disorder, remains one of the foremost public health challenges affecting more than 30 million people worldwide with etiology still largely enigmatic. The intricate gut-brain axis, serving as vital communication network between gut and brain, appears to wield influence in progression AD. Our study showcases remarkable precision x-ray phase-contrast tomography (XPCT) conducting an advanced three-dimensional examination cellular...
Abstract The investigation of the neuronal network in mouse spinal cord models represents basis for research on neurodegenerative diseases. In this framework, quantitative analysis single elements different districts is a crucial task. However, conventional 3D imaging techniques do not have enough spatial resolution and contrast to allow network. Exploiting high coherence flux synchrotron sources, X-ray Phase-Contrast multiscale-Tomography allows microanatomy without any aggressive sample...
Abstract A collection of more than 1800 carbonized papyri, discovered in the Roman ‘Villa dei Papiri’ at Herculaneum is unique classical library survived from antiquity. These papyri were charred during 79 A.D. Vesuvius eruption, a circumstance which providentially preserved them until now. This magnificent contains an impressive amount treatises by Greek philosophers and, especially, Philodemus Gadara, Epicurean thinker 1st century BC. We read many portions text hidden inside using enhanced...
We report a method that allows complete quantitative characterization of whole single cells, assessing the total amount carbon, nitrogen, oxygen, sodium, and magnesium providing submicrometer maps element molar concentration, cell density, mass, volume. This approach quantifying elements down to 106 atoms/μm3. result was obtained by applying multimodal fusion combines synchrotron radiation microscopy techniques with off-line atomic force microscopy. The proposed permits us find concentration...
We present here a correlative X-ray microscopy approach for quantitative single cell imaging of molar concentrations. By combining the elemental content provided by fluorescence and morphology information extracted from phase nanotomography, we determine intracellular molarity distributions. This method was demonstrated on freeze-dried human phagocytic to obtain absolute concentration maps K, P, Fe. The results showed very good agreement with atomic-force measurements. work opens way...
Only a few Herculaneum rolls exhibit writing on their reverse side. Since unrolled papyri are permanently glued to paperboard, so far, this fact was known us only from 18th-century drawings. The application of shortwave-infrared (SWIR; 1000-2500 nm) hyperspectral imaging (HSI) one them (PHerc. 1691/1021) has revealed portions Greek text hidden the back more than 220 years after first discovery, making it possible recover primary source for ongoing new edition precious book. SWIR HSI produced...
Diffraction and refraction phenomena at the entrance of a hollow x-ray waveguide with weakly absorbing dielectric cladding layers are investigated using two independent approaches: (a) analytical (b) numerical solutions wave equation in paraxial (parabolic) approximation. It is shown that penetrating through material substantially modifies field near entrance. results significant increase total energy flux inside guiding layer additional spatial modulation electromagnetic field.
Computed x-ray phase contrast micro-tomography is the most valuable tool for a three dimensional (3D) and non destructive analysis of tissue engineered bone morphology. We used Talbot interferometer installed at SYRMEP beamline ELETTRA synchrotron (Trieste, Italy) precise 3D reconstruction both soft connective tissue, regenerated in vivo within porous scaffold. For first time tomographic reconstructions have been combined with scanning micro-diffraction measurement on same sample, order to...
Abstract Visualization and characterization of $$\beta$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>β</mml:mi></mml:math> -amyloid deposits is a fundamental task in pre-clinical study Alzheimer’s disease (AD) to assess its evolution monitor the efficiency new therapeutic strategies. While cerebellum one brain areas most underestimated context AD, renewed interest cerebellar lesions has recently arisen as they may link motor cognitive alterations. Thus, we quantitatively...
Abstract The 3D complexity of biological tissues and intricate structural-functional connections call for state-of-the-art X-ray imaging approaches to overcome limitations classical imaging. Unlike other techniques, phase-contrast tomography (XPCT) offers a highly sensitive approach investigate different disease-relevant networks at levels ranging from single cell through intact organ. We present here concomitant study the evolution tissue damage inflammation in organs affected by disease...
A three-image method to extract absorption, refraction and scattering information for hard x-ray grating interferometry is presented. The comprises a post-processing approach alternative the conventional phase stepping procedure inspired by similar technique developed analyzer-based imaging. Results obtained with this algorithm are quantitatively comparable phase-stepping. This can be further extended samples negligible scattering, where only two images needed separate absorption signal....
The coupling and propagation of electromagnetic waves through planar X-ray waveguides (WG) with vacuum gap Si claddings are analyzed in detail, starting from the source ending at detector. general case linearly tapered WGs (i.e. entrance aperture different exit one) is considered. Different kinds sources, i.e. synchrotron radiation laboratory desk-top have been considered, former providing a fully coherent incoming beam latter partially beams. It demonstrated that useful information about...
We report a qualitative study on central nervous system (CNS) damage that demonstrates the ability of X-ray phase contrast tomography (XPCT) to confirm data obtained with standard 2D methodology and permits description additional features are not detected or other 3D techniques. In magnetic resonance computed tomography, XPCT makes possible high-resolution imaging soft tissues classically considered “invisible” X-rays without use agents, need for intense processing tissue required by Most...
Abstract Ancient Herculaneum papyrus scrolls, hopelessly charred in the 79 A.D. Vesuvius eruption, contain valuable writings of Greek philosophers day, including works Epicurean Philodemus. X-ray phase contrast tomography has recently begun unlocking their secrets. However, only small portions text hidden inside scroll have been recover. One challenging tasks papyri investigation is virtual unfolding because highly complicated structure and three-dimensional arrangement. Although this...
We report the performance of an X-ray phase contrast microscope for laboratory sources with 300 nm spatial resolution. The is based on a commercial microfocus source equipped planar waveguide able to produce sub-micrometer x-ray beam in one dimension. Phase images representative samples are reported. achieved and resolution discussed different configurations. proposed approach could represent simple, inexpensive, solution imaging small setups.