A5083818461- Adipose Tissue and Metabolism
- Magnesium in Health and Disease
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Plant Micronutrient Interactions and Effects
- Nutrition and Health in Aging
- Metabolism, Diabetes, and Cancer
- Muscle metabolism and nutrition
- Trace Elements in Health
- Aluminum toxicity and tolerance in plants and animals
- Amino Acid Enzymes and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Metabolism and Genetic Disorders
- Cardiovascular and exercise physiology
- Ion Transport and Channel Regulation
- Plant nutrient uptake and metabolism
- Force Microscopy Techniques and Applications
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Fatty Acid Research and Health
- Cardiovascular Function and Risk Factors
- Mitochondrial Function and Pathology
- Vitamin C and Antioxidants Research
- Exercise and Physiological Responses
Deutsches Diabetes-Zentrum e.V.
2018-2025
Heinrich Heine University Düsseldorf
2018-2025
German Center for Diabetes Research
2018-2025
Freie Universität Berlin
2013-2024
Università Cattolica del Sacro Cuore
2009-2011
Weatherford College
2008
Epidemiological studies have shown that increased circulating branched-chain amino acids (BCAAs) are associated with insulin resistance and type 2 diabetes (T2D). This may result from altered energy metabolism or dietary habits. We hypothesized a lower intake of BCAAs improves tissue-specific sensitivity. randomized, placebo-controlled, double-blinded, crossover trial examined well-controlled T2D patients receiving isocaloric diets (protein: 1 g/kg body weight) for 4 wk. Protein requirements...
Disturbed hepatic energy metabolism contributes to non-alcoholic fatty liver (NAFLD), but the development of changes over time and obesity- or diabetes-related mechanisms remained unclear.Two-day old male C57BL/6j mice received streptozotocin (STZ) placebo (PLC) then high-fat (HFD) regular chow diet (RCD) from week 4 (W4) either W8 W16, yielding control [CTRL = PLC + RCD], diabetes [DIAB STZ obesity [OBES HFD] steatohepatitis [NASH models. Mitochondrial respiration was measured by...
The athlete’s paradox states that intramyocellular triglyceride accumulation associates with insulin resistance in sedentary but not endurance-trained humans. Underlying mechanisms and the role of muscle lipid distribution composition on glucose metabolism remain unclear. We compared highly trained athletes (ATHL) normal weight (LEAN) overweight-to-obese (OVWE) male female individuals. This observational study found ATHL show higher sensitivity, mitochondrial content, capacity, lower...
Abstract The mechanisms underlying improved insulin sensitivity after surgically-induced weight loss are still unclear. We monitored skeletal muscle metabolism in obese individuals before and over 52 weeks metabolic surgery. Initial occurs parallel with a decrease oxidative capacity respiratory control ratio. Persistent elevation of intramyocellular lipid intermediates, likely resulting from unrestrained adipose tissue lipolysis, accompanies the lack rapid changes sensitivity....
OBJECTIVE Individuals with type 2 diabetes are at higher risk of progression nonalcoholic fatty liver (steatosis) to steatohepatitis (NASH), fibrosis, and cirrhosis. The hepatic metabolism obese individuals adapts by upregulation mitochondrial capacity, which may be lost during the steatosis. However, role regard function in NASH remains unclear. RESEARCH DESIGN AND METHODS We therefore examined histologically proven without (OBE) (n = 30; BMI 52 ± 9 kg/m2) or (T2D) 15; 51 7 as well healthy...
Parkinson's disease (PD) is a complex multifactorial ailment predetermined by the interplay of various environmental and genetic factors. Systemic intracellular magnesium (Mg) deficiency has long been suspected to contribute development progress PD other neurodegenerative diseases. However, molecular background unknown. Interestingly, gene SLC41A1 located in novel locus PARK16 recently identified as being Na+/Mg2+ exchanger (NME, Mg2+ efflux system), key component cellular homeostasis. Here,...
Abstract The important role of magnesium (Mg 2+ ) in normal cellular physiology requires flexible, yet tightly regulated, intracellular Mg homeostasis (IMH). However, only little is known about transporters subcellular compartments such as mitochondria, despite their obvious importance for the deposition and reposition pools. In particular, knowledge mechanisms responsible extrusion from mitochondria lacking. Based on circumstantial evidence, two possible release were predicted: (1) efflux...
SEV may serve as new biomarkers for predicting the individual response of insulin sensitivity to exercising.
Hepatic and myocardial ectopic lipid deposition has been associated with insulin resistance (IR) cardiovascular risk. Lipid overload promotes increased hepatic oxidative capacity, stress, impaired mitochondrial efficiency, driving the progression of nonalcoholic fatty liver disease (NAFLD). We hypothesized that higher availability ischemia-induced cardiac dysfunction decreases efficiency. Mice adipose tissue–specific overexpression sterol element–binding protein 1c as model combined NAFLD-IR...
Abstract Aims/hypothesis Energy-dense nutrition generally induces insulin resistance, but dietary composition may differently affect glucose metabolism. This study investigated initial effects of monounsaturated vs saturated lipid meals on basal and insulin-stimulated myocellular metabolism signalling. Methods In a randomised crossover study, 16 lean metabolically healthy volunteers received single containing safflower oil (SAF), palm (PAL) or vehicle (VCL). Whole-body was assessed from...
Highlights•Glycemia and liver lipid content jointly affect the adaptation of hepatic oxidative capacity to insulin resistance•Prediabetes affects signaling, mitochondrial dynamics relates fibrosis prevalence•Fasting plasma glucose predicts decline plasticity more robustly than 2-hour OGTT glucoseAbstractBackground & AimsHepatic respiration is higher in steatosis, but lower overt type 2 diabetes. We hypothesized that OXPHOS increases with a greater degree resistance obesity, independent other...
Mitochondrial Mg channel MRS2 overexpression influences concentration and sensitivity to apoptosis.
Hyperbaric oxygen (HBO) therapy may improve hyperglycaemia in humans with type 2 diabetes, but underlying mechanisms are unclear. Our objective was to examine the glucometabolic effects of HBO on whole-body glucose disposal diabetes.In a randomised placebo-controlled crossover trial located at German Diabetes Center, 12 male individuals diabetes (age 18-75 years, BMI <35 kg/m2, HbA1c 42-75 mmol/mol [6-9%]), randomly allocated by one person, underwent 2-h HBO, once 100% (240 kPa; HBO) and 21%...
Abstract Mammary epithelial cells (HC11) chronically adapted to grow in a low‐magnesium (0.05 mM vs. 0.5 mM) or high‐magnesium (40 medium were used investigate on the mechanisms of cell magnesium transport under conditions non‐physiological availability. Magnesium influx was higher compared control cells, whereas efflux and cells. partially inhibited by imipramine, inhibitor Na + /Mg 2+ exchange. Using monoclonal antibody detecting ∼70 kDa protein associated with exchange activity, we found...
The Na+/Mg2+ exchanger SLC41A1 (A1), a key component of intracellular Mg homeostasis (IMH), is the major cellular Mg2+ efflux system, and its overexpression decreases [Mg2+]intracellular. IMH plays an important role in regulation many processes, including signaling. However, whether A1 consequent drop [Mg2+]i impact on signaling unknown. To examine latter, we utilized dynamic mass redistribution (DMR) assay, PathScan® RTK antibody (PRSA) array, confirmatory Western blot (WB) analyses...