A5022774436- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Chemokine receptors and signaling
- Ferroptosis and cancer prognosis
- Immune Response and Inflammation
- Nanoplatforms for cancer theranostics
- Monoclonal and Polyclonal Antibodies Research
- Phagocytosis and Immune Regulation
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Lymphoma Diagnosis and Treatment
- Intraperitoneal and Appendiceal Malignancies
- Inflammasome and immune disorders
- Nanoparticle-Based Drug Delivery
- Cancer Genomics and Diagnostics
- HER2/EGFR in Cancer Research
- Neuroinflammation and Neurodegeneration Mechanisms
- MicroRNA in disease regulation
- Autoimmune and Inflammatory Disorders Research
- Histone Deacetylase Inhibitors Research
- Ovarian cancer diagnosis and treatment
- Epigenetics and DNA Methylation
- Inflammatory Bowel Disease
Automation and Process Engineering Laboratory
2023-2024
Université Claude Bernard Lyon 1
2023-2024
Centre National de la Recherche Scientifique
2023-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2013-2024
University of Messina
2024
Agostino Gemelli University Polyclinic
2024
Policlinico Abano Terme
2024
Institut National d'Oncologie
2024
Hospices Civils de Lyon
2024
Ospedale Policlinico San Martino
2024
Tumor-promoted constraints negatively affect cytotoxic T lymphocyte (CTL) trafficking to the tumor core and, as a result, inhibit killing. The production of reactive nitrogen species (RNS) within microenvironment has been reported in mouse and human cancers. We describe novel RNS-dependent posttranslational modification chemokines that profound impact on leukocyte recruitment tumors. Intratumoral RNS induces CCL2 chemokine nitration hinders cell infiltration, resulting trapping...
Local delivery of anticancer agents has the potential to maximize treatment efficacy and minimize acute long-term systemic toxicities. Here, we used unsupervised systematic evolution ligands by exponential enrichment identify four RNA aptamers that specifically recognized mouse human myeloid cells infiltrating tumors but not their peripheral or circulating counterparts in multiple models from patients with head neck squamous cell carcinoma (HNSCC). The use these conjugated doxorubicin...
Myeloid Derived suppressor cells (MDSCs) play a key role in the progression and recurrence of human malignancies restraining efficacy adjuvant therapies. We have previously shown that Tadalafil lowers MDSCs regulatory T (Treg) blood tumor, primes tumor specific immune response, increases number activated intratumoral CD8+T patients with primary Head Neck Squamous Cell Carcinoma (HNSCC). However, despite these important modulatory actions, to date no clinically significant effects been...
Cancer-induced 'emergency' myelopoiesis plays a key role in tumor progression by inducing the accumulation of myeloid cells with suppressive phenotype peripherally and tumor. Chemokine receptors (CCRs) and, particular, CCR1, CCR2, CCR5, CCR7 are emerging as regulators cell trafficking function but their precise has not been completely clarified yet because signal redundancy, integration, promiscuity chemokines expression these CCRs on other leukocyte subsets.We used 4PD nanoparticle for vivo...
Conventional therapies for inflammatory bowel diseases are mainly based on systemic treatments which cause side effects and toxicity over long-term administration. Nanoparticles appear as a valid alternative to allow preferential accumulation in inflamed tissues following oral administration while reducing drug exposure. To increase their residence time the intestine, nanoparticles here associated with hydrogel matrix. A bioadhesive peptide-based is mixed nanoemulsions, creating hybrid...
Abstract Emerging evidence suggests that not only the frequency and composition of tumor-infiltrating leukocytes but also their spatial organization might be a major determinant tumor progression response to therapy. Therefore, mapping analyzing fine immune architecture could potentially provide insights for predicting cancer prognosis. Here, we performed an explorative, prospective clinical study assess whether structures within microenvironment can predict recurrence after salvage surgery...
The ability to detect and target β cells in vivo can substantially refine how diabetes is studied treated. However, the lack of specific probes still hampers a precise characterization human cell mass delivery therapeutics clinical settings. Here, we report identification two RNA aptamers that specifically selectively recognize mouse cells. putative targets are transmembrane p24 trafficking protein 6 (TMED6) clusterin (CLUS). When given systemically immune deficient mice, these islet graft...
Abstract Myeloid cells play a key role in tumor progression and metastasis by providing nourishment immune protection, as well facilitating cancer invasion seeding to distal sites. Although advances have been made understanding the biology of these tumor-educated myeloid (TEMCs), their intrinsic plasticity challenges our further biology. Indeed, vitro experiments only mimic vivo setting, current gene-knockout technologies do not allow simultaneous, temporally controlled, cell-specific...
Abstract Inflammatory responses rapidly detect pathogen invasion and mount a regulated reaction. However, dysregulated anti-pathogen immune can provoke life-threatening inflammatory pathologies collectively known as cytokine release syndrome (CRS), exemplified by key clinical phenotypes unearthed during the SARS-CoV-2 pandemic. The underlying pathophysiology of CRS remains elusive. We found that FLIP, protein controls caspase-8 death pathways, was highly expressed in myeloid cells COVID-19...
Abstract Inflammatory responses rapidly detect pathogen invasion and mount a regulated reaction. However, dysregulated anti-pathogen immune can provoke life-threatening inflammatory pathologies collectively known as cytokine release syndrome (CRS), exemplified by key clinical phenotypes unearthed during the SARS-Cov-2 pandemic. The underlying pathophysiology of CRS remains elusive. We found that FLIP, protein controls caspase-8 death pathways, was highly expressed in myeloid cells COVID-19...
Abstract Introduction: Depending on their polarization, myeloid cells can either promote or restrain tumor progression. By secreting myelopoietic factors tumours polarization toward a phenotype that provides immune protection and stimulate neoplastic growth, invasiveness, metastasis. Chemokines cognate receptors are thought to play key role in cell trafficking, however, study vivo is hindered by signal redundancy integration. Methods: taking advantage of newly developed nanoplatform allows...
<p>Numeric data describing a) sample composition, analysis parameters, and phenotyping; b) phenotype composition of the merged dataset each single sample; c) cell type (number cells) cellular neighborhoods identified at W=10 K=14; d) Number cells assigned to 11 e): relative cell-cell contact frequencies (RF) for all phenotypes in TLS1 TLS2 CNs across patients; f) hot cold tumor patients</p>
<p>supplementary figure S1-4</p>