A5024465077- Protein Degradation and Inhibitors
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Ovarian cancer diagnosis and treatment
- Chromatin Remodeling and Cancer
- Cancer-related Molecular Pathways
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Cancer-related gene regulation
- Renal and related cancers
- Cytokine Signaling Pathways and Interactions
- Ubiquitin and proteasome pathways
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Estrogen and related hormone effects
- Pluripotent Stem Cells Research
- Cancer Mechanisms and Therapy
- Virus-based gene therapy research
- FOXO transcription factor regulation
- PI3K/AKT/mTOR signaling in cancer
- Endometrial and Cervical Cancer Treatments
- Cervical Cancer and HPV Research
- RNA modifications and cancer
- 3D Printing in Biomedical Research
NewYork–Presbyterian Brooklyn Methodist Hospital
2020-2024
Cornell University
2001-2024
Weill Cornell Medicine
2020-2024
University of Miami
2011-2020
Sylvester Comprehensive Cancer Center
2011-2020
Miami Transplant Institute
2018
Brigham and Women's Hospital
2003-2017
Clinical Pharmacology of Miami
2016
Whitehead Institute for Biomedical Research
2002-2014
Harvard University
2002-2013
Fatty acid binding protein 4 (FABP4) plays an important role in maintaining glucose and lipid homeostasis. FABP4 has been primarily regarded as adipocyte- macrophage-specific protein, but recent studies suggest that it may be more widely expressed. We found strong expression the endothelial cells (ECs) of capillaries small veins several mouse human tissues, including heart kidney. was also detected ECs mature placental vessels infantile hemangiomas, most common tumor infancy ECs. In these...
Heat-shock factor 1 (HSF1) is the master transcriptional regulator of cellular response to heat and a wide variety other stressors. We previously reported that HSF1 promotes survival proliferation malignant cells. At this time, however, clinical prognostic significance in cancer unknown. To address issue breast samples from 1,841 participants Nurses’ Health Study were scored for levels nuclear HSF1. Associations status with parameters outcomes investigated by Kaplan–Meier analysis Cox...
Dissemination of ovarian tumors involves the implantation cancer spheroids into mesothelial monolayer on walls peritoneal and pleural cavity organs. Biopsies attached to organs show that cells are not present under tumor masses. We have developed a live, image-based in vitro model which interactions between can be monitored real time provide spatial temporal understanding clearance. Here we evidence utilize integrin- talin- dependent activation myosin traction force promote displacement from...
Abstract Background Recent interest in reference-free deconvolution of DNA methylation data has led to several supervised methods, but these methods do not easily permit the interpretation underlying cell types. Results We propose a simple method for that provides both proportions putative types defined by their methylomes, number constituent types, as well evaluating extent which methylomes reflect specific cells. demonstrate an analysis 23 Infinium sets from 13 distinct collection efforts;...
NUT midline carcinoma (NMC) is a lethal pediatric tumor defined by the presence of BRD-NUT fusion proteins that arrest differentiation. Here we explore mechanisms underlying ability BRD4-NUT to prevent squamous In both gain-of and loss-of-expression assays, find expression associated with globally decreased histone acetylation transcriptional repression. Bulk chromatin can be restored treatment NMC cells deacetylase inhibitors (HDACi), engaging program differentiation arrested growth in...
Accurate classification is essential for understanding the pathophysiology of a disease and can inform therapeutic choices. For hematopoietic malignancies, scheme based on phenotypic similarity between tumor cells normal has been successfully used to define subtypes; however, use cell types as reference by which classify solid tumors not widely emulated, in part due more limited epithelial differentiation compared with hematopoiesis. To provide better definition subtypes comprising breast...
Missense mutations in the gene TP53 , which encodes p53, one of most important tumor suppressors, are common human cancers. Accumulated mutant p53 proteins known to actively contribute development and metastasis. Thus, promoting removal cancer cells may have therapeutic significance. Here we investigated mechanisms that govern turnover nonproliferating using a combination pharmacological genetic approaches. We show suppression macroautophagy by multiple means promotes degradation through...
Abstract Currently available human tumour cell line panels consist of a small number lines in each lineage that generally fail to retain the phenotype original patient tumour. Here we develop culture medium enables us routinely establish from diverse subtypes ovarian cancers with >95% efficiency. Importantly, 25 new described here genomic landscape, histopathology and molecular features tumours. Furthermore, profile drug response these correlate distinct groups primary tumours different...
Abstract Consequences of the obesity epidemic on cancer morbidity and mortality are not fully appreciated. Obesity is a risk factor for many cancers, but mechanisms by which it contributes to development patient outcome have yet be elucidated. Here, we examined effects coculturing human-derived adipocytes with established primary breast cells tumorigenic potential. We found that interaction between increased secretion proinflammatory cytokines. Prolonged culture or cytokines proportion...
Tumours are comprised of a highly heterogeneous population cells, which only small subset stem-like cells possess the ability to regenerate tumours in vivo. These cancer stem (CSCs) represent significant clinical challenge as they resistant conventional therapies and play essential roles metastasis tumour relapse. Despite this realization great interest CSCs, it has been difficult develop CSC-targeted treatments due our limited understanding CSC biology. Here, we present evidence that...
p27 restrains normal cell growth, but PI3K-dependent C-terminal phosphorylation of at threonine 157 (T157) and T198 promotes cancer invasion. Here, we describe an oncogenic feedforward loop in which p27pT157pT198 binds Janus kinase 2 (JAK2) promoting STAT3 (signal transducer activator transcription 3) recruitment activation. induces TWIST1 to drive a p27-dependent epithelial-mesenchymal transition (EMT) further activates AKT contributing acquisition maintenance metastatic potential....
To investigate the mechanism that drives dramatic mistargeting of active chromatin in NUT midline carcinoma (NMC), we have identified protein interactions unique to BRD4-NUT fusion oncoprotein compared with wild-type BRD4. Using cross-linking, affinity purification, and mass spectrometry, EP300 acetyltransferase as uniquely associated BRD4 through both NMC non-NMC cell types. We also discovered ZNF532 patient cells but not detectable 293T cells. are implicated feed-forward regulatory loops...
Metastatic dissemination of ovarian tumors involves the invasion tumor cell clusters into mesothelial lining peritoneal cavity organs; however, tumor-specific factors that allow cancer cells to spread are unclear. We used an in vitro assay models initial step metastasis, clearance layer, examine ability a large panel both established and primary cells. Comparison gene protein expression profiles clearance-competent clearance-incompetent revealed mesenchymal genes enriched populations display...