A5000755236- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Assisted Reproductive Technology and Twin Pregnancy
- Renal and related cancers
- Ovarian function and disorders
- Reproductive System and Pregnancy
- CRISPR and Genetic Engineering
- Reproductive Health and Technologies
- Epigenetics and DNA Methylation
- Sperm and Testicular Function
- Prenatal Screening and Diagnostics
- Animal Genetics and Reproduction
- Ectopic Pregnancy Diagnosis and Management
- Endometriosis Research and Treatment
- Zebrafish Biomedical Research Applications
- RNA Research and Splicing
- Gynecological conditions and treatments
- Ovarian cancer diagnosis and treatment
- Metabolism and Genetic Disorders
- Uterine Myomas and Treatments
- Neurogenetic and Muscular Disorders Research
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
Stemcell Technologies
2024-2025
Dana-Farber Cancer Institute
2006-2014
Harvard Stem Cell Institute
2006-2013
Boston Children's Hospital
2008-2013
Harvard University
2006-2012
Advanced Medical Research Institute
2012
Brigham and Women's Hospital
2006-2010
Howard Hughes Medical Institute
2010
Michigan State University
2006
Kindai University
1999-2004
Genetically matched pluripotent embryonic stem (ES) cells generated via nuclear transfer or parthenogenesis (pES cells) are a potential source of histocompatible and tissues for transplantation. After parthenogenetic activation murine oocytes interruption meiosis I II, we isolated genotyped pES characterized those that carried the full complement major histocompatibility complex (MHC) antigens oocyte donor. Differentiated from these engrafted in immunocompetent MHC-matched mouse recipients,...
The clinical application of human adipose-derived mesenchymal stem cells (MSCs) as treatment for intractable diseases or traumatic tissue damage has attracted attention. To address the ability reactivating injured ovaries, we prepared a rat model with damaged ovaries by using an anticancer agent, cyclophosphamide (CTX). We then investigated restorative effects on ovarian function and safety MSCs (A-MSCs). were shown to be capable inducing angiogenesis restoring number follicles corpus lutea...
Abstract LIN28A/B are RNA binding proteins implicated by genetic association studies in human growth and glucose metabolism. Mice with ectopic over-expression of Lin28a have shown related phenotypes. Here, we describe the first comprehensive analysis physiologic consequences Lin28b deficiency knockout (KO) mice. Lin28a/b-deficiency led to dwarfism starting at different ages, compound gene deletions showed a cumulative dosage effect on organismal growth. Conditional deletion specific...
The scarcity of primordial germ cells (PGCs) in the developing mammalian embryo hampers robust biochemical analysis processes that underlie early cell formation. Here, we demonstrate DAZL, a cell-specific RNA binding protein, is PGC marker during vitro development. Using Dazl-GFP reporter ESCs, DAZL plays central role large mRNA/protein interactive network blocks translation core pluripotency factors, including Sox2 and Sall4, as well Suz12, polycomb family member required for...
To study the intrinsic fertility of human oocyte.A large retrospective natural cycle single embryo transfer (ET) IVF cycles.Private clinic, university, and private hospital.Patients were enrolled consecutively over an 8-year period in a ET protocol.A total 13,949 oocyte retrievals with ET. Software package R (version 3.2.5) was used for statistical calculations.Live baby rate per according to age.A 14,185 oocytes resulted 1,913 live babies from The number required make one this series varied...
Cdx genes ( Cdx1 , Cdx2 and Cdx4 ) encode a family of caudal -related transcription factors that mediate anterior–posterior patterning during embryogenesis through Hox gene regulation. Homologues in the zebrafish have been shown to play key roles blood formation. To define role embryonic hematopoiesis mammalian system, we examined hematopoietic potential -deficient mouse stem cells (ESCs) vitro vivo . Individual ESCs exhibited impaired progenitor formation altered expression, most notably...
Abstract Overexpression of LIN28A is associated with human germ cell tumors and promotes primordial (PGC) development from embryonic stem cells in vitro chimeric mice. Knockdown Lin28a inhibits PGC vitro, but how constitutional deficiency affects the mammalian reproductive system vivo remains unknown. Here, we generated knockout (KO) mice found that compromises size pool both males females by affecting proliferation during embryogenesis. Interestingly however, KO males, partially recovers...
Ovarian stimulation induced by follicle-stimulating hormone and human chorionic gonadotrophin (hCG) is commonly used in assisted reproductive technology to increase embryo production. However, recent clinical animal studies have shown that ovarian disrupts endometrial function development adversely affects pregnancy outcomes. How impairs establishment the precise mechanisms which this reduces chances of conception remain unclear. In study, we first demonstrated using hCG alone implantation,...
Cryopreservation of mature oocytes and embryos has provided numerous benefits in reproductive medicine. Although successful cryopreservation germinal-vesicle stage (GV) holds promise for further advances biology clinical embryology fields, reports regarding immature are limited. Oocyte survival maturation rates have improved since vitrification is being performed at the GV stage, but subsequent developmental competence still low. The purpose this study was to evaluate effects supplementation...
Are there any differences in live birth rates (LBR) following fresh blastocyst transfer natural or clomiphene-stimulated cycles, after elective freezing cycles followed by thawing and at different time-points? Clomiphene citrate (CC) administration adversely affected the LBR single (SBT) CC compared with that while this adverse effect of is not present when a vitrified-warmed (SVBT) performed subsequent ovulatory regardless duration between day SVBT. affects uterine receptivity associated...