A5017057954- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Hematological disorders and diagnostics
- Bone and Joint Diseases
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Zebrafish Biomedical Research Applications
- Immune Cell Function and Interaction
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
- Drug Transport and Resistance Mechanisms
- Chronic Lymphocytic Leukemia Research
- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Lymphatic System and Diseases
- Neonatal Respiratory Health Research
- T-cell and B-cell Immunology
- Cancer, Hypoxia, and Metabolism
- Cell Image Analysis Techniques
- Microfluidic and Bio-sensing Technologies
- Hormonal Regulation and Hypertension
- CRISPR and Genetic Engineering
- Pomegranate: compositions and health benefits
- Histiocytic Disorders and Treatments
University of Lausanne
2018-2025
École Polytechnique Fédérale de Lausanne
2012-2024
University Hospital of Lausanne
2012-2024
University of Wisconsin–Madison
2024
Universitat de Barcelona
2024
National Institute on Aging
2024
Hôpital Orthopédique de la Suisse Romande
2017
Harvard Stem Cell Institute
2005-2015
Harvard University
2003-2015
Dana-Farber Cancer Institute
2009-2015
Haematopoietic stem cells (HSCs) differ from their committed progeny by relying primarily on anaerobic glycolysis rather than mitochondrial oxidative phosphorylation for energy production. However, whether this change in the metabolic program is cause or consequence of unique function HSCs remains unknown. Here we show that enforced modulation metabolism impacts HSC self-renewal. Lowering activity chemically uncoupling electron transport chain drives self-renewal under culture conditions...
Despite two decades of studies documenting the in vitro blood-forming potential murine embryonic stem cells (ESCs), achieving stable long-term blood engraftment ESC-derived hematopoietic irradiated mice has proven difficult. We have exploited Cdx-Hox pathway, a genetic program important for development, to enhance differentiation ESCs along lineage. Using an cell line engineered with tetracycline-inducible Cdx4 , we demonstrate that ectopic expression promotes mesoderm specification,...
Blood flow promotes emergence of definitive hematopoietic stem cells (HSCs) in the developing embryo, yet signals generated by hemodynamic forces that influence potential remain poorly defined. Here we show fluid shear stress endows long-term multilineage engraftment upon early tissues at embryonic day 9.5, an stage not previously described to harbor HSCs. Effects on hematopoiesis are mediated part a cascade downstream wall involves calcium efflux and stimulation prostaglandin E2...
The treatment of bone defects with recombinant morphogenetic protein-2 (BMP-2) requires high doses precluding broad clinical application. Here, a bioengineering approach is presented that strongly improves low-dose BMP-2-based regeneration by mobilizing healing-associated mesenchymal progenitor cells (MPCs). Smart synthetic hydrogels are used to trap and study endogenous MPCs trafficking defects. Hydrogel-trapped prospectively isolated differentiate into multiple lineages in vitro form vivo....
Aging is the major risk factor for most human diseases and represents a socio-economical challenge modern societies. Despite its importance, process of aging remains poorly understood. Epigenetic dysregulation has been proposed as key driver process. Modifications in transcriptional networks chromatin structure might be central to age-related functional decline. A prevalent feature described during overall reduction heterochromatin, specifically marked by loss repressive histone...
The in vitro expansion of long-term hematopoietic stem cells (HSCs) remains a substantial challenge, largely because our limited understanding the mechanisms that control HSC fate choices. Using single-cell multigene expression analysis and time-lapse microscopy, here we define gene signatures cell cycle hallmarks murine HSCs earliest multipotent progenitors (MPPs), analyze systematically single choices culture. Our revealed twelve differentially expressed genes marking quiescent state,...
The bone marrow (BM) exists heterogeneously as hematopoietic/red or adipocytic/yellow depending on skeletal location, age, and physiological condition. Mouse models patients undergoing radio/chemotherapy suffering acute BM failure endure rapid adipocytic conversion of the microenvironment, so-called "red-to-yellow" transition. Following hematopoietic recovery, such upon transplantation, a "yellow-to-red" transition occurs functional hematopoiesis is restored. Gold Standards to estimate...
Abstract A novel type of injectable biomaterial with an elastic softening transition is described. The material enables in vivo shaping, followed by induction 3D stable vascularized tissue. synthesis the meta‐biomaterial instructed extensive numerical simulation as a suspension irregularly fragmented, highly porous sponge‐like microgels. irregular particle shape dramatically enhances yield strain for stability against deformation. Porosity particles, along friction between internal surfaces,...
Response to MEK inhibition with trametinib and tyrosine kinase
Bone marrow adipocytes (BMAds) constitute the most abundant stromal component of adult human bone marrow. Two subtypes BMAds have been described, more labile regulated (rBMAds) and stable constitutive (cBMAds), which develop earlier in life are resilient to environmental metabolic disruptions. In vivo, rBMAds enriched saturated fatty acids, contain smaller lipid droplets (LDs) readily provide hematopoietic support than their cBMAd counterparts. Mouse models used for research, but isolation...
In vitro differentiating adipocytes are sensitive to liquid manipulations and have the tendency float. Assessing adipocyte differentiation using current microscopy techniques involves cell staining washing, while flow cytometry retrieval in suspension. These methods induce biases, difficult reproduce, involve tedious optimizations. this study, we present digital holographic (DHM) as a label-free, nonperturbing means quantify lipid droplets robust medium- high-throughput manner. Taking...