A5022683596- T-cell and B-cell Immunology
- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Muscle Physiology and Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Cell Adhesion Molecules Research
- Fermentation and Sensory Analysis
- Horticultural and Viticultural Research
- Chemokine receptors and signaling
- Atherosclerosis and Cardiovascular Diseases
- Genomics, phytochemicals, and oxidative stress
- Calcium signaling and nucleotide metabolism
- Biochemical and biochemical processes
- Cytomegalovirus and herpesvirus research
- Hepatitis B Virus Studies
- Pancreatic function and diabetes
- Immune cells in cancer
- Analytical chemistry methods development
- Hepatitis Viruses Studies and Epidemiology
- Extracellular vesicles in disease
- Monoclonal and Polyclonal Antibodies Research
- Adipose Tissue and Metabolism
- Connective Tissue Growth Factor Research
- Xenotransplantation and immune response
Nestlé (Switzerland)
2016-2025
Agroscope
2022-2024
École Polytechnique Fédérale de Lausanne
2016-2023
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2015
Vita-Salute San Raffaele University
2010-2014
San Raffaele University of Rome
2013-2014
Istituti di Ricovero e Cura a Carattere Scientifico
2014
Azienda Ospedaliero Universitaria San Giovanni Battista
2005-2009
University of Turin
2001-2007
Diurnal oscillations of gene expression controlled by the circadian clock and its connected feeding rhythm enable organisms to coordinate their physiologies with daily environmental cycles. While available techniques yielded crucial insights into regulation at transcriptional level, much less is known about temporally functions within nucleus protein level. Here, we quantified temporal nuclear accumulation proteins phosphoproteins from mouse liver SILAC proteomics. We identified around 5,000...
A major challenge in unrelated hematopoietic stem cell transplantation (HSCT) is the prediction of permissive HLA mismatches, ie, those associated with lower clinical risks compared to their nonpermissive counterparts. For HLA-DPB1, a clinically prognostic model has been shown be matching for T epitope (TCE) groups assigned by cross reactivity cells alloreactive HLA-DPB1∗09:01; however, molecular basis this observation not fully understood. Here, we have mutated amino acids (aa) 10 positions...
Skeletal muscle is a regenerative tissue which can repair damaged myofibers through the activation of tissue-resident stem cells (MuSCs). Many diseases with impaired regeneration cause excessive adipose accumulation in muscle, alter myogenic fate MuSCs, and deregulate cross-talk between MuSCs fibro/adipogenic progenitors (FAPs), bi-potent cell population supports myogenesis controls intra-muscular fibrosis adipocyte formation. In order to better characterize interaction adipogenesis...
Nicotinamide adenine dinucleotide (NAD+), a precursor of molecules involved in cell regulatory processes, is released extracellular compartments after stress or inflammation. This study investigates the expression human cornea CD38 and CD157, two NAD+-consuming ectoenzymes surface receptors. The analysis corneal epithelial stromal cells was performed by means multiple approaches, which included immunofluorescence, reverse transcriptase polymerase chain reaction (RT-PCR), Western blot,...
Regulation of mitochondrial redox balance is emerging as a key event for cell signaling in both physiological and pathological conditions. However, the link between state modulation these conditions remains poorly defined. Here, we discovered that activation evolutionary conserved calcium uniporter (MCU) modulates state. By using mitochondria-targeted sensors genetic MCU-ablated models, provide evidence causality MCU net reduction (but not cytosolic) Redox redox-sensitive groups via...
Postbiotics are defined as a "preparation of inanimate microorganisms and/or their components that confers health benefit on the host". They represent an attractive alternative to probiotics they could be used in broader range applications, where probiotic stability is limiting. To date knowledge mechanism action relatively scarce. In this study, we investigated impact heat treatment NRF2 activation by several candidate strains from Nestlé Culture Collection (NCC), including species...
Increasing evidence suggests that the muscle stem cell (MuSC) pool is heterogeneous. In particular, a rare subset of PAX7-positive MuSCs has never expressed myogenic regulatory factor MYF5 displays unique self-renewal and engraftment characteristics. However, scarcity limited availability protein markers make characterization these cells challenging. Here, we describe generation StemRep reporter mice enabling monitoring PAX7 proteins based on equimolar levels dual nuclear fluorescence. High...
Polymorphisms in the 3′ untranslated region (3′UTR) of HLA‐G, an important player immunological tolerance, could be involved post‐transcriptional expression control, and their association with different clinical immune‐related conditions including autoimmunity transplantation is mounting interest. Most studies have focused on a 14 base pair (bp) insertion/deletion (ins/del), while additional single‐nucleotide polymorphisms (SNPs) HLA‐G 3′UTR been described but not extensively investigated...
The maintenance or expansion of regulatory T (Treg) cells has a fundamental role in the achievement immunological tolerance after transplantation. Here we aimed to determine mechanisms human Treg cell depletion and reconstitution anti-CD25 monoclonal antibody (mAb) treatment.Seventeen patients with type 1 diabetes who received pancreatic islet transplantation mAb as induction therapy were studied.We observed an almost complete injection mAb. kinetic did not parallel disappearance CD25+ CD25...
The production of reactive oxygen species (ROS) may promote immunosenescence if not counterbalanced by the antioxidant systems. Cell membranes, proteins, and nucleic acids become target ROS progressively lose their structure functions. This process could lead to an impairment immune response. However, little is known about capability cells elderly individuals dynamically counteract oxidative stress. Here, response main lymphocyte subsets induced stress in semisupercentenarians (CENT),...
We explored the relation between blood concentrations of monocyte/lymphocyte subsets and carotid artery plaque macrophage content, measured by positron emission tomography (PET) with 11C-PK11195.In 9 patients plaques we performed 11C-PK11195-PET/computed angiography imaging measurement absolute frequencies circulating monocytes T-cell subsets. Plaque standardized uptake value (SUV) for 11C-PK11195 was negatively correlated total (r = -0.58, p 0.05) CD14++CD16-HLA-DR+ classical subset -0.82,...
The purpose of this study was to develop a novel, objective, and semiautomated method quantify conjunctival redness by correlating measured with standard clinical symptom scales inflammatory cell infiltration.Eleven outpatients presenting mild severe hyperemia were included in the study. Clinical examination patient history; visual analogue score (VAS) for ocular symptoms; 25-item National Eye Institute Visual Function Questionnaire (NEI-VFQ 25) quality life/vision; photographs anterior...
We developed and validated a reliable, robust, easy-to-implement quantitative method for multielemental analysis of low-volume samples. Our ICP-MS-based comprises the 20 elements (Mg, P, S, K, Ca, V, Cr, Mn, Fe, Co, Cu, Zn, Se, Br, Rb, Sr, Mo, I, Cs, Ba) in 10 μL serum 12 Zn Cs) less than 250 000 cells. As proof-of-concept, we analyzed elemental profiles sorted immune T cells derived from naı̈ve tumor-bearing mice. The results indicate tumor systemic effect on both approach highlights...
The development of cell-based biosensors that give insight into cell and tissue function in vivo is an attractive technology for biomedical research. Here, the a line expressing fluorescent calcium sensor study beta-cell reported. bioresponsive model based on INS-1E pancreatic beta-cells, stably genetically encoded cameleon-based YC3.6cyto . Following single-cell selection expansion, functional testing vitro encapsulation experiments are used to identify suitable clone cells sensor. This...
Steady hematopoiesis is essential for lifelong production of all mature blood cells. Hematopoietic stem and progenitor cells (HSPCs) found in the bone marrow ensure hematopoietic homeostasis an organism. Failure this complex process, which involves a fine balance self-renewal differentiation fates, often result severe hematological conditions such as leukemia lymphoma. Several molecular metabolic programs, internal or close interaction with niche, have been identified important regulators...