A5079296388- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Sirtuins and Resveratrol in Medicine
- Tissue Engineering and Regenerative Medicine
- Genetics, Aging, and Longevity in Model Organisms
- Telomeres, Telomerase, and Senescence
- Parathyroid Disorders and Treatments
- Mesenchymal stem cell research
- Neurobiology and Insect Physiology Research
- Pluripotent Stem Cells Research
- Exercise and Physiological Responses
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Connective Tissue Growth Factor Research
École Polytechnique Fédérale de Lausanne
2019-2024
Nestlé (Switzerland)
2019-2024
Baxter (United States)
2021
Stanford University
2021
Duchenne muscular dystrophy (DMD) is a rare X-linked recessive disease that associated with severe progressive muscle degeneration culminating in death due to cardiorespiratory failure. We previously observed an unexpected proliferation-independent telomere shortening cardiomyocytes of DMD mouse model. Here, we provide mechanistic insights using human induced pluripotent stem cell-derived (hiPSC-CMs). Using traction force microscopy, show hiPSC-CMs exhibit deficits generation on...
Increasing evidence suggests that the muscle stem cell (MuSC) pool is heterogeneous. In particular, a rare subset of PAX7-positive MuSCs has never expressed myogenic regulatory factor MYF5 displays unique self-renewal and engraftment characteristics. However, scarcity limited availability protein markers make characterization these cells challenging. Here, we describe generation StemRep reporter mice enabling monitoring PAX7 proteins based on equimolar levels dual nuclear fluorescence. High...
Skeletal muscle relies on resident stem cells (MuSCs) for growth and repair. Aging diseases impair MuSC function, leading to cell exhaustion regenerative decline that contribute the progressive loss of skeletal mass strength. In absence clinically available nutritional solutions specifically targeting MuSCs, we used a human myogenic progenitor (hMP) high-content imaging screen natural molecules from food identify nicotinamide (NAM) pyridoxine (PN) as bioactive nutrients stimulate MuSCs have...
Nicotinamide riboside kinases (NRKs) control the conversion of dietary Riboside (NR) to NAD+, but little is known about their contribution endogenous NAD+ turnover and muscle plasticity during skeletal growth remodeling. Using NRK1/2 double KO (NRKdKO) mice, we investigated influence NRKs on metabolism homeostasis, response neurogenic atrophy regeneration following injury. Muscles from NRKdKO animals have altered nicotinamide (NAM) salvage a decrease in mitochondrial content. In single...
Summary Increasing evidence suggests heterogeneity in the muscle stem cell (MuSC) pool. In particular, a rare subset of Pax7 positive MuSCs that has never expressed myogenic regulatory factor Myf5 enhanced self-renewal and engraftment characteristics. However, scarcity limited availability protein markers make characterization these cells challenging. We describe generation StemRep reporter mice allowing to monitor based on equimolar levels dual nuclear fluorescence. High low delineate...