A5020185605- Alzheimer's disease research and treatments
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Dementia and Cognitive Impairment Research
- Traumatic Brain Injury and Neurovascular Disturbances
- RNA modifications and cancer
- S100 Proteins and Annexins
- Cancer-related gene regulation
- Anorectal Disease Treatments and Outcomes
- Advanced Biosensing Techniques and Applications
- Traumatic Brain Injury Research
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Asthma and respiratory diseases
- Trauma and Emergency Care Studies
- HIV/AIDS Research and Interventions
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- COVID-19 Clinical Research Studies
- Health Promotion and Cardiovascular Prevention
- Health, psychology, and well-being
- HIV/AIDS drug development and treatment
- Nerve Injury and Rehabilitation
- Appendicitis Diagnosis and Management
- Liver physiology and pathology
Quanterix (United States)
2015-2024
Ottawa Hospital Research Institute
2014-2024
Ottawa Hospital
2014-2024
Institute of Genetics and Cancer
2018-2023
University of Edinburgh
2019-2023
Centre for Inflammation Research
2023
Campbell Collaboration
2021
Thomas Jefferson University
2021
MRC Human Genetics Unit
2018-2020
University College London
2019
Disease detection at the molecular level is driving emerging revolution of early diagnosis and treatment. A challenge facing field that protein biomarkers for can be present in very low abundance. The lower limit with conventional immunoassay technology upper femtomolar range (10–13 M). Digital has improved sensitivity three logs, to attomolar (10–16 This capability potential open new advances diagnostics therapeutics, but such technologies have been relegated manual procedures are not well...
Compared with patients mild cognitive impairment (MCI) or controls, P <0.001. b MCI, P = 0.001. c P = 0.02. AD, Alzheimer’s disease;
Cholangiocarcinoma (CC) is typically diagnosed at an advanced stage and refractory to surgical intervention chemotherapy. Despite a global increase in the incidence of CC, little progress has been made toward development treatments for this cancer. Here we utilized human tissue; CC cell xenografts; p53-deficient transgenic mouse model; non-transgenic, chemically induced rat model that accurately reflects both inflammatory regenerative background associated with pathology. Using these...
We report a method for combining the detection of single molecules (digital) and an ensemble (analog) that is capable detecting enzyme label from 10−19 M to 10−13 M, use in high sensitivity enzyme-linked immunosorbent assays (ELISA). The approach works by capturing proteins on microscopic beads, labeling with enzymes using conventional multistep immunosandwich approach, isolating beads array 50-femtoliter wells (Single Molecule Array, SiMoA), bead-associated enzymatic activity fluorescence...
Glial fibrillary acidic protein (GFAP), microtubule-associated tau, and amyloid β peptide (Aβ42) have been proposed as diagnostic prognostic biomarkers in traumatic brain injury (TBI). Single molecule array (Simoa) is a novel technology that employs highly sensitive immunoassays for accurate measurements of candidate found at low concentration biological fluids. Our objective was to trace the trajectory GFAP, Aβ42 levels plasma from acute through subacute stages after TBI, compared with...
Abstract The COVID-19 pandemic continues to have an unprecedented impact on societies and economies worldwide. There remains ongoing need for high-performance SARS-CoV-2 tests which may be broadly deployed infection monitoring. Here we report a highly sensitive single molecule array (Simoa) immunoassay in development detection of nucleocapsid protein (N-protein) venous capillary blood saliva. In all matrices the studies conducted date observe >98% negative percent agreement >90%...
The role of microglia in the removal amyloid deposits after systemically administered anti-Aβ antibodies remains unclear. In current study, we injected Tg2576 APP transgenic mice weekly with an antibody for 1, 2, or 3 months such that all were 22 at end study. immunized months, found improvement alternation performance Y maze. Histologically, able to detect mouse IgG bound congophilic those treated but not a control antibody. We Fcγ receptor expression on was increased 1 month treatment,...
Amyloid β (Aβ) peptides are proteolytic products from amyloid precursor protein (APP) and thought to play a role in Alzheimer disease (AD) pathogenesis. While much is known about molecular mechanisms underlying cerebral Aβ accumulation familial AD, less the cause(s) of brain amyloidosis sporadic disease. Animal postmortem studies suggest that secretion can be up-regulated response hypoxia. We employed new technology (Single Molecule Arrays, SiMoA) capable ultrasensitive measurements...
In 2005, the International Diabetes Federation (IDF) released a consensus clinical definition of metabolic syndrome for worldwide use that included central obesity as prerequisite (1). The IDF varied from earlier Third Report U.S. National Cholesterol Education Program Adult Treatment Panel (ATP III) panel with broader criteria waist circumference, HDL, and fasting plasma glucose (2). impact these changes to prevalence syndrome, in particular differing values defining obesity, has not been...
We report a method for isolating individual paramagnetic beads in arrays of femtolitre-sized wells and detecting single enzyme-labeled proteins on these using sequential fluid flows microfabricated polymer array assemblies. Arrays were fabricated cyclic olefin (COP) injection moulding based DVD manufacturing. These bonded to complementary fluidic structure that was also moulded COP create an enclosed device allow delivery liquids the arrays. Enzyme-associated, suspended aqueous solutions...
Twenty pairs of patients matched for sex, age, weight, and degree trauma were treated either with an active Diapulse machine emitting pulsed, high-frequency electrical energy or a non-active placebo machine. They examined swelling, pain, disability before treatment after three days under double-blind conditions. The results show that the has definite biological effect on recently injured soft tissues, especially in reduction pain disability.
Traumatic brain injury (TBI) results in heterogeneous pathology affecting multiple cells and tissue types the brain. It is likely that assessment of such complexity will require simultaneous measurement molecular biomarkers a single sample biological fluid. We measured glial fibrillary acidic protein (GFAP), ubiquitin c-terminal hydrolase L1 (UCH-L1), neurofilament light chain (NF-L) total tau plasma samples obtained from 107 subjects enrolled Transforming Research Clinical Knowledge Brain...
The aim of the study was to examine ability eight protein biomarkers and their combinations in discriminating computed tomography (CT)-negative CT-positive patients with traumatic brain injury (TBI), utilizing highly sensitive immunoassays a well-characterized cohort. Blood samples were obtained from 160 acute TBI within 24 h admission. Levels β-amyloid isoforms 1-40 (Aβ40) 1-42 (Aβ42), glial fibrillary acidic (GFAP), heart fatty-acid binding (H-FABP), interleukin 10 (IL-10), neurofilament...
The Men Androgen Inflammation Lifestyle Environment and Stress (MAILES) Study was established in 2009 to investigate the associations of sex steroids, inflammation, environmental psychosocial factors with cardio-metabolic disease risk men. study population consists 2569 men from harmonisation two studies: all participants Florey Adelaide Male Ageing (FAMAS) eligible male North West Health (NWAHS). cohort has so far participated three stages MAILES Study: MAILES1 (FAMAS Wave 1, 2002-2005,...
The purpose of this study was to correlate the early levels glial fibrillary acidic protein (GFAP) and neurofilament light (NF-L) with outcome in patients mild traumatic brain injury (mTBI). A total 107 mTBI (Glasgow Coma Scale ≥13) who had blood samples for GFAP NF-L available within 24 h arrival were included. Patients divided into computed tomography (CT)-positive CT-negative groups. Glasgow Outcome Scale-Extended (GOSE) used assess outcome. Outcomes defined as complete (GOSE 8) versus...
Detection of acute HIV infection is critical for public health and diagnostics. Clinical fourth-generation antigen (Ag)/antibody (Ab) combination (combo) p24 Ag immunoassays have enhanced detection compared to Ab-alone assays but require ongoing evaluation with currently circulating diverse subtypes.
Abstract The number of patients diagnosed with chronic bile duct disease is increasing and in most cases these diseases result ductular scarring, necessitating liver transplantation. formation scaring affects function; however, scar-generating portal fibroblasts also provide important instructive signals to promote the proliferation differentiation biliary epithelial cells. Therefore, understanding whether we can reduce scar while maintaining a pro-regenerative microenvironment will be...
Abstract Objectives Neurofilament light chain (NfL) has emerged as a promising biomarker for detecting and monitoring axonal injury. Until recently, NfL could only be reliably measured in cerebrospinal fluid, but digital single molecule array (Simoa) technology enabled its precise measurement blood samples where it is typically 50–100 times less abundant. We report development multi-center validation of novel fully automated immunoassay serum informing injury status. Methods A 45-min was...