A5026244795- Prion Diseases and Protein Misfolding
- Alzheimer's disease research and treatments
- Dementia and Cognitive Impairment Research
- Neurological diseases and metabolism
- Trace Elements in Health
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- RNA Research and Splicing
- Parkinson's Disease Mechanisms and Treatments
- RNA regulation and disease
- S100 Proteins and Annexins
- Acute Myeloid Leukemia Research
- Amyotrophic Lateral Sclerosis Research
- Glycosylation and Glycoproteins Research
- Iron Metabolism and Disorders
- Mass Spectrometry Techniques and Applications
- Retinoids in leukemia and cellular processes
- Neurological Disease Mechanisms and Treatments
- Health, Environment, Cognitive Aging
- Multiple Sclerosis Research Studies
- Erythropoietin and Anemia Treatment
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- Cerebrospinal fluid and hydrocephalus
Inserm
2016-2025
Université de Montpellier
2016-2025
Karolinska Institutet
2009-2025
Centre Hospitalier Universitaire de Montpellier
2015-2024
Centre National de la Recherche Scientifique
2009-2024
Université de Nîmes
2022-2024
Institute for Neurosciences of Montpellier
2019-2024
Hôpital Saint Eloi
2013-2024
Institute for Regenerative Medicine & Biotherapy
2015-2024
Uppsala University
2022-2024
The cellular prion protein PrP c is a glycosylphosphatidylinositol-anchored cell-surface whose biological function unclear. We used the murine 1C11 neuronal differentiation model to search for -dependent signal transduction through antibody-mediated cross-linking. A caveolin-1–dependent coupling of tyrosine kinase Fyn was observed. Clathrin might also contribute this coupling. ability cell line trigger activation restricted its fully differentiated serotonergic or noradrenergic progenies....
Direct reprogramming of somatic cells into induced pluripotent stem (iPSCs) provides a unique opportunity to derive patient-specific with potential applications in tissue replacement therapies and without the ethical concerns human embryonic (hESCs). However, cellular senescence, which contributes aging restricted longevity, has been described as barrier derivation iPSCs. Here we demonstrate, using an optimized protocol, that senescence is not limit age-related physiology reversible. Thus,...
Abstract Capsaicin is the major pungent ingredient in red peppers. Here, we report that it has a profound antiproliferative effect on prostate cancer cells, inducing apoptosis of both androgen receptor (AR)-positive (LNCaP) and -negative (PC-3, DU-145) cell lines associated with an increase p53, p21, Bax. down-regulated expression not only prostate-specific antigen (PSA) but also AR. Promoter assays showed capsaicin inhibited ability dihydrotestosterone to activate PSA promoter/enhancer even...
Background and objective: We explored which clinical biochemical variables predict conversion from clinically isolated syndrome (CIS) to definite multiple sclerosis (CDMS) in a large international cohort. Methods: Thirty-three centres provided serum samples 1047 CIS cases with at least two years’ follow-up. Age, sex, presentation, T2-hyperintense lesions, cerebrospinal fluid (CSF) oligoclonal bands (OCBs), CSF IgG index, cell count, 25-hydroxyvitamin D3 (25-OH-D), cotinine titres against...
BackgroundAlzheimer's disease and its complications are the leading cause of death in adults with Down syndrome. Studies have assessed Alzheimer's individuals syndrome, but natural history biomarker changes syndrome has not been established. We characterised order timing biomarkers a population syndrome.MethodsWe did dual-centre cross-sectional study recruited through population-based health plan Barcelona (Spain) services for people intellectual disabilities Cambridge (UK). Cognitive...
Abstract Background Cerebrospinal fluid biomarker profiles characterized by decreased amyloid-beta peptide levels and increased total phosphorylated tau at threonine 181 (pT181) are currently used to discriminate between Alzheimer’s disease other neurodegenerative diseases. However, these changes not entirely specific disease, it is noteworthy that isoforms of tau, possibly more for the process, have been described in brain parenchyma patients. The precise detection biological fluids remains...
Prion diseases are transmissible neurodegenerative disorders affecting humans and animals for which no therapeutic or prophylactic regimens exist. During the last three years several studies have shown that anti-PrP monoclonal antibodies (mAbs) can antagonize prion propagation in vitro vivo, but mechanisms of inhibition not known so far. To identify most powerful mAbs characterize more precisely effect antibodies, we screened 145 different produced our laboratory their capacity to cure cells...
ABSTRACT Propagation of the agents responsible for transmissible spongiform encephalopathies (TSEs) in cultured cells has been achieved only a few cell lines. To establish efficient and versatile models transmission, we developed neuroblastoma lines overexpressing type A mouse prion protein, MoPrP C -A, then tested susceptibility to several different mouse-adapted scrapie strains. The transfected clones expressed up sixfold-higher levels PrP than untransfected cells. Even after 30 passages,...
The molecular mechanism of neurodegeneration in transmissible spongiform encephalopathies remains uncertain. In this study, it was demonstrated that prion-infected hypothalamic neuronal GT1 cells displayed a higher sensitivity to induced oxidative stress over noninfected cells. addition, the infected presented an increased lipid peroxidation and signs apoptosis associated with dramatic reduction activities glutathione-dependent superoxide dismutase antioxidant systems. This study indicates...
Transmissible spongiform encephalopathies, or prion diseases, are fatal degenerative disorders of the central nervous system that affect humans and animals. Prions nonconventional infectious agents whose replication depends on host protein (PrP). Transmission prions to cultured cells has proved be a particularly difficult task, with few exceptions, their experimental propagation relies inoculation laboratory Here, we report development permanent cell line supporting natural sheep scrapie....
Management and traceability of biospecimen preanalytical variations are necessary to provide effective efficient interconnectivity interoperability between Biobanks.Therefore, the International Society for Biological Environmental Repositories Biospecimen Science Working Group developed a "Standard PREanalytical Code" (SPREC) that identifies main factors clinical fluid solid biospecimens their simple derivatives.The SPREC is easy implement can be integrated into Biobank quality management...
Over the last 30 years, many drugs have been tested both in cell culture and vivo for their ability to prevent generation of prions development transmissible spongiform encephalopathies. Among compounds tested, dendrimers are defined by branched repeating molecular structure. The anti-prion activity new cationic phosphorus-containing (P-dendrimers) with tertiary amine end-groups was tested. These molecules had a strong activity, decreasing PrP Sc infectivity scrapie-infected cells at...
Background: Despite sensitivity of MRI to diagnose multiple sclerosis (MS), prognostic biomarkers are still needed for optimized treatment. Objective: The objective this paper is identify cerebrospinal fluid (CSF) diagnostic MS using quantitative proteomics and analyze their expression at different disease stages. Methods: We conducted differential analysis the CSF proteome from control relapsing–remitting (RRMS) patients followed by verification ELISA candidate in serum control, clinically...