A5028625543- Cell Adhesion Molecules Research
- Advanced Proteomics Techniques and Applications
- Protease and Inhibitor Mechanisms
- Proteoglycans and glycosaminoglycans research
- Molecular Biology Techniques and Applications
- Ovarian cancer diagnosis and treatment
- Monoclonal and Polyclonal Antibodies Research
- Sarcoma Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Cellular Mechanics and Interactions
- Gene expression and cancer classification
- Advanced Biosensing Techniques and Applications
- Immune Response and Inflammation
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- S100 Proteins and Annexins
- Peptidase Inhibition and Analysis
- Galectins and Cancer Biology
- Immune Cell Function and Interaction
- Radiopharmaceutical Chemistry and Applications
- Neuropeptides and Animal Physiology
- Clusterin in disease pathology
- Wnt/β-catenin signaling in development and cancer
- Heat shock proteins research
- Renal and related cancers
University of Minnesota
2016-2025
University of Minnesota Medical Center
2009-2025
Women's Health Research Institute
2019
Cancer Research UK Manchester Institute
2010
Moffitt Cancer Center
2010
Masonic Cancer Center
1993-2008
Emory University Hospital
2000
Palmetto Hematology Oncology
1999
Wayne State University
1997
University of Manchester
1995
Immunohistochemical (IHC) assays performed on formalin-fixed paraffin-embedded (FFPE) tissue sections traditionally have been semi-quantified by pathologist visual scoring of staining. IHC is useful for validating biomarkers discovered through genomics methods as large clinical repositories FFPE specimens support the construction microarrays (TMAs) high throughput studies. Due to ubiquitous availability techniques in laboratories, validated may be translated readily into use. However, method...
Tissue-specific gene expression in mammary epithelium is dependent on the extracellular matrix as well hormones. There good evidence that basement membrane provides signals for regulating beta-casein expression, and integrins are involved this process. Here, we demonstrate presence of lactogenic hormones, laminin can direct gene. Mouse epithelial cells plated gels native or laminin-entactin undergo functional differentiation. On tissue culture plastic, respond to soluble purified laminin,...
Management and traceability of biospecimen preanalytical variations are necessary to provide effective efficient interconnectivity interoperability between Biobanks.Therefore, the International Society for Biological Environmental Repositories Biospecimen Science Working Group developed a "Standard PREanalytical Code" (SPREC) that identifies main factors clinical fluid solid biospecimens their simple derivatives.The SPREC is easy implement can be integrated into Biobank quality management...
Early detection of ovarian cancer is difficult owing to the lack specific and sensitive tests available. Previously, we found expression nectin 4 be increased in compared with normal ovaries. Reverse transcriptase-polymerase chain reaction (RT-PCR) quantitative RT-PCR validated overexpression messenger RNA cell lines tissues. Protein levels were elevated tissue as demonstrated by Western immunoblotting, flow cytometry, immunohistochemical staining microarray slides. Cleaved was detectable a...
The first version of the Standard PREanalytical Code (SPREC) was developed in 2009 by International Society for Biological and Environmental Repositories (ISBER) Biospecimen Science Working Group to facilitate documentation communication most important preanalytical quality parameters different types biospecimens used research. This same has now updated SPREC 2.0, presented here, so that it contains more options allow recent technological developments. Existing elements have been fine tuned....
Cellular recognition and adhesion to the extracellular matrix (ECM) has a complex molecular basis, involving both integrins cell surface proteoglycans (PG). The current studies have used specific inhibitors of chondroitin sulfate proteoglycan (CSPG) synthesis along with anti-alpha 4 integrin subunit monoclonal antibodies demonstrate that human melanoma an A-chain derived, 33-kD carboxyl-terminal heparin binding fragment plasma fibronectin (FN) involves CSPG alpha beta 1 integrin. A direct...
Recent studies using solid-phase-binding assays and electron microscopy suggested the presence of a heparin-binding domain between inner globule lateral short arm cross region laminin. Using information from amino acid sequence B1 chain laminin, several peptides were synthesized areas with low hydropathy index high density lysines and/or arginines. One these, peptide F-9 (RYVVLPRPVCFEKGMNYTVR), which is derived globular arm, demonstrated specific binding to heparin. This was tested in direct...
Malignant ascites often develops in advanced stages of ovarian carcinoma, consisting single and aggregated tumor cells, or spheroids. Spheroids have commonly been used as models to study drug efficacy, shown resistance some chemotherapies radiation. However, little is known about the adhesive invasive capabilities spheroids, whether this particular cellular component can contribute dissemination cancer. Here, we examined ability spheroids recovered from seven carcinoma patients one primary...
The purpose of this study was to identify the binding site(s) within laminin for alpha 3 beta 1 integrin receptor. It has been previously shown, using proteolytic fragments and anti-laminin antibodies, that region in is localized carboxy-terminal at end long arm (Gehlsen, K. R., E. Engvall, Dickerson, W. S. Argraves, Ruoslahti. 1989. J. Biol. Chem. 264:19034-19038; Tomaselli, J., D. Hall, L. T. Reichardt, A. Flier, R. Gehlsen, C. Turner, Carbonetto. 1990. Neuron. 5:651-662). Using synthetic...
Cell adhesion to extracellular matrix components such as fibronectin has a complex basis, involving multiple determinants on the molecule that react with discrete cell surface macromolecules. Our previous results have demonstrated normal and transformed cells adhere spread 33-kD heparin binding fragment originates from carboxy-terminal end of particular isoforms (A-chains) human fibronectin. This promotes melanoma spreading in an arginyl-glycyl-aspartyl-serine (RGDS) independent manner,...
Abstract Four members of the earcinoembryonic antigen family, CD66a, CD66b, CD66c, and CD66d, are expressed on human neutrophils. In neutrophils these proteins activation antigens in that their surface expression is increased following stimulation. To examine potential role neutrophil signaling, effects adhesion to umbilical vein endothelial cells a panel well-characterized CD66 mAbs was tested. CD66d antibodies each cell monolayers. This increase caused by blocked CD18 antibody associated...
The adhesion and motility of tumor cells on basement membranes is a central consideration in cell invasion metastasis. Basement membrane type IV collagen directly promotes the migration various types vitro. Our previous studies demonstrated that adhered spread surfaces coated with intact or either two major enzymatically purified domains this protein. Only one these domains, pepsin-generated triple helical fragment, also supported vitro, implicating involvement region collagen-mediated vivo....
Abstract Background New biomarkers that replace or are used in conjunction with the current ovarian cancer diagnostic antigen, CA125, needed for detection of presurgical setting, as well disease recurrence. We previously demonstrated upregulation leucine-rich alpha-2-glycoprotein-1 (LRG1) sera patients compared to healthy women using quantitative mass spectrometry. Methods LRG1 was quantified by ELISA serum from two relatively large cohorts and benign gynecological disease. The expression...