A5032234273- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Atherosclerosis and Cardiovascular Diseases
- Cell Adhesion Molecules Research
- HIV Research and Treatment
- Chemokine receptors and signaling
- HIV/AIDS drug development and treatment
- Protein Degradation and Inhibitors
- Lymphoma Diagnosis and Treatment
- Eosinophilic Disorders and Syndromes
- Ubiquitin and proteasome pathways
- HIV/AIDS Research and Interventions
- Viral Infections and Immunology Research
- Chronic Lymphocytic Leukemia Research
- Ocular Infections and Treatments
- Acute Ischemic Stroke Management
- Cardiovascular Health and Disease Prevention
- Extracellular vesicles in disease
- Bacteriophages and microbial interactions
- Acute Myeloid Leukemia Research
- Ocular Surface and Contact Lens
- Acute Lymphoblastic Leukemia research
- Respiratory viral infections research
- Immune cells in cancer
- Glaucoma and retinal disorders
IRCCS Ospedale San Raffaele
2022-2025
Vita-Salute San Raffaele University
2000-2025
San Raffaele University of Rome
2011-2023
Nestlé (Switzerland)
2015
Ruhr University Bochum
2000
The SDF-1 3'A allelic polymorphism has been reported to influence either positively or negatively the progression of human immunodeficiency virus type 1 (HIV-1) disease. Therefore, genotype 729 HIV-1-infected individuals pooled from 3 distinct cohorts was determined. A statistically nonsignificant association between SDF1-3'A/3'A and accelerated disease evident among seroconverters (n=319), but a striking correlation decreased survival after diagnosis AIDS according 1993 definition loss...
ABSTRACT Extracellular vesicles (EVs) are crucial for intercellular communication and found in various biological fluids. The identification immunophenotyping of such small particles continue to pose significant challenges. Here, we have developed a workflow the optimisation next‐generation panel in‐depth circulating plasma EVs using spectral flow cytometry. Our data collection followed multistep phase both instrument setup 21‐colour design, thus maximising fluorescent signal recovery. This...
We explored the relation between blood concentrations of monocyte/lymphocyte subsets and carotid artery plaque macrophage content, measured by positron emission tomography (PET) with 11C-PK11195.In 9 patients plaques we performed 11C-PK11195-PET/computed angiography imaging measurement absolute frequencies circulating monocytes T-cell subsets. Plaque standardized uptake value (SUV) for 11C-PK11195 was negatively correlated total (r = -0.58, p 0.05) CD14++CD16-HLA-DR+ classical subset -0.82,...
The purpose of this study was to develop a novel, objective, and semiautomated method quantify conjunctival redness by correlating measured with standard clinical symptom scales inflammatory cell infiltration.Eleven outpatients presenting mild severe hyperemia were included in the study. Clinical examination patient history; visual analogue score (VAS) for ocular symptoms; 25-item National Eye Institute Visual Function Questionnaire (NEI-VFQ 25) quality life/vision; photographs anterior...
Summary. The purging efficacy of the Miltenyi sorting system was evaluated by qualitative and TaqMan quantitative polymerase chain reaction (PCR) in myeloma patients, using immunoglobulin genes. After small‐scale selection, PCR showed that 6 12 leukaphereses cells were no longer detectable. Envisaging a possible clinical application, from three patients underwent large‐scale selection. Qualitative still Quantitative PCR, performed two tumour depletion of␣1 2 logs respectively. Although...
We describe a multi-step high-dimensional (HD) flow cytometry workflow for the deep phenotypic characterization of T cells infiltrating metastatic tumor lesions in liver, particularly derived from colorectal cancer (CRC-LM). First, we applied novel cytometer setting approach based on single positive rather than fluorescent beads, resulting optimal sensitivity when compared with previously published protocols. Second, set up 26-color antibody panel designed to assess functional state both...
Recombinant MVAs (rMVAs) are widely used both in basic and clinical research. Our previously developed Red-to-Green Gene Swapping Method (RGGSM), a cytometry-based Cell-Sorting protocol, revolves around the transient expression of green fluorescent cytoplasmic marker, to subsequently obtain purified untagged rMVA upon loss that marker by site-specific recombination. The standard RGSSM is quite costly terms bench work, reagents, Sorting Facility fees. Although faster than other methods...
Extracellular vesicles (EVs) released by mesenchymal stromal cells (MSCs) contain a set of microRNAs with regenerative and anti-inflammatory roles. Therefore, purified MSC-EVs are envisioned as next-generation therapeutic option for wide array diseases. In this protocol, we report the strategy successfully sorting EVs from supernatant adipose-derived MSCs (ASCs), often used in orthopedics medicine applications. First, described sample preparation, focusing on EV isolation labeling steps...
Abstract Background Recombinant MVAs (rMVAs) are widely used both in basic and clinical research. Our previously developed Red-to-Green Gene Swapping Method (RGGSM), a cytometry-based Cell-Sorting protocol, revolves around the transient expression of green fluorescent cytoplasmic marker, to subsequently obtain purified untagged rMVA upon loss that marker by site-specific recombination. The standard RGSSM is quite costly terms bench work, reagents, Sorting Facility fees. Although faster than...