A5050549426- Immune Cell Function and Interaction
- HIV Research and Treatment
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- Viral-associated cancers and disorders
- Immunotherapy and Immune Responses
- Herpesvirus Infections and Treatments
- HIV/AIDS drug development and treatment
- Parvovirus B19 Infection Studies
- HIV/AIDS Research and Interventions
- Immunodeficiency and Autoimmune Disorders
- Pancreatic function and diabetes
- Systemic Lupus Erythematosus Research
- Reproductive System and Pregnancy
- Essential Oils and Antimicrobial Activity
- Plant Pathogens and Fungal Diseases
- Cervical Cancer and HPV Research
- Glycosylation and Glycoproteins Research
- HIV-related health complications and treatments
- Virus-based gene therapy research
- Diabetes and associated disorders
- Multiple Myeloma Research and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Muscle Physiology and Disorders
- Viral gastroenteritis research and epidemiology
IRCCS Ospedale San Raffaele
1991-2024
Istituti di Ricovero e Cura a Carattere Scientifico
1997-2024
Vita-Salute San Raffaele University
2005-2022
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
1997-2022
San Raffaele University of Rome
2003-2018
Regione Puglia
2011
National Institutes of Health
1991-2009
Department of Virology
2009
Université Laval
2009
Sorbonne Université
2009
Recognition by natural killer (NK) cells of major histocompatibility complex (MHC) class I molecules on target inhibits NK-mediated lysis. Here, inhibition NK clones HLA-B*2705 mutated at single amino acids in the peptide binding site varied among HLA-B*2705-specific clones. In addition, a subset such was inhibited only one several self peptides loaded onto expressed transporter-deficient cells, showing that recognition peptide-specific. These data demonstrate specific peptides, complexed...
The aim of this study was to examine the effect five naturally occurring compounds from essential oils on 10 different species mycotoxigenic fungi involved in several plant diseases. antifungal activities terpinen-4-ol, eugenol, carvone, 1,8-cineole (eucalyptol) and thymol were observed vitro Fusarium subglutinans, cerealis, verticillioides, proliferatum, oxysporum, sporotrichioides, Aspergillus tubingensis, carbonarius, Alternaria alternata Penicillium sp. tested showed toxic effects...
Background Neutralizing antibodies provide markers for vaccine-induced protective immunity in many viral infections. By analogy, HIV-1 neutralizing induced by immunization may well predict vaccine effectiveness. Assessment of is therefore primary importance, but hampered the fact that we do not know which assay(s) can measures immunity. An international collaboration (NeutNet) involving 18 different laboratories previously compared assays using monoclonal (mAbs) and soluble CD4 (Phase I...
NK clones that were inhibited by target cell expression of HLA-B*2705 displayed peptide-specific recognition HLA-B*2705. To evaluate the specificity this recognition, synthetic versions 14 endogenous ligands tested for their ability to provide protection from NK-mediated lysis binding surface molecules on RMA-S cells deficient in transporter Ag presentation. Several unrelated peptides same clones. Despite similar capacities stabilize cells, differed widely abilities protection. Single amino...
ABSTRACT The diagnosis of human herpesvirus 6 (HHV-6) infection represents a complex issue because the most widely used diagnostic tools, such as immunoglobulin G antibody titer determination and qualitative DNA PCR with blood cells, are unable to distinguish between latent (clinically silent) active (often clinically relevant) infection. We have developed new, highly sensitive, quantitative assay for accurate measurement HHV-6 in tissue-derived cell suspensions body fluids. test uses 5′...
Abstract The susceptibility to infection by human herpes-virus 6 (HHV-6) of mature T lymphocytes belonging the two major subpopulations (i.e., CD3+ CD4+ CD8- and CD4- CD8+) was investigated using or CD8+ cell populations clones derived from normal adult peripheral blood. Productive HHV-6 observed in both cells. By days 2 after infection, increasing numbers cells exhibited characteristic morphologic alterations, becoming enlarged, uniformly rounded refractile as a consequence virus-induced...
Aims: The aim of this study was to examine the effect Melaleuca alternifolia essential oil (TTO) and its principal components on four cereal-pathogenic fungi. Methods Results: antimycotic properties TTO terpinen-4-ol, γ-terpinen 1,8-cineole (eucalyptol) were evaluated in vitro Fusarium graminearum, culmorum Pyrenophora graminea. Moreover, barley leaves infected with Blumeria graminis treated whole TTO. All tested fungi susceptible components. Conclusions: exerted a wide spectrum activity....
Background Neutralizing antibody assessments play a central role in human immunodeficiency virus type-1 (HIV-1) vaccine development but it is unclear which assay, or combination of assays, will provide reliable measures correlates protection. To address this, an international collaboration (NeutNet) involving 18 independent participants was organized to compare different assays. Methods Each laboratory evaluated four neutralizing reagents (TriMab, 447-52D, 4E10, sCD4) at given range...
Human CD3- lymphocyte populations were obtained by treating peripheral blood lymphocytes with mAbs directed to CD3, CD4, and CD8 surface antigens. The resulting cultured irradiated allogeneic cells; at day 4, 100 U/ml IL-2 added cultures continued for an additional 10 d. CD3-CD2+CD7+ displayed cytolytic activity against PHA-induced blast cells bearing the stimulating alloantigens but not autologous or unrelated cells. When cells, no could be detected either On other hand, K562 target lysed...
Objectives: During HIV-1 infection, the development, phenotype, and functionality of B cells are impaired. Transitional aberrant B-cell populations arise in blood, whereas a declined percentage resting memory is detected. Our study aimed at pinpointing demographic, immunological, viral factors driving these pathological findings, role antiretroviral therapy reverting alterations. Design: phenotype correlating were evaluated. Methods: Variations subsets evaluated by flow cytometry...
Despite repeated exposure to HIV-1, certain individuals remain persistently uninfected. Such exposed uninfected (EU) people show evidence of HIV-1–specific T cell immunity and, in rare cases, selective resistance infection by macrophage-tropic strains HIV-1. The latter has been associated with a 32–base pair deletion the C–C chemokine receptor gene CCR-5, major coreceptor We have undertaken an analysis HIV-specific responses 12 EU who were either homozygous for wild-type CCR-5 allele or...
The natural Ag influenza virus A was used to test the requirements for HLA-DR1-restricted presentation of epitopes 18-29 in matrix protein and 307-318 hemagglutinin protein. CD4+ cytotoxic T cell clones similar efficiency were detect these two epitopes. Presentation epitope by APC pulsed with either inactivated particles or purified soluble followed classical pathway that 1) it required invariant chain expression, 2) blocked inhibition synthesis, 3) dependent on a function(s) encoded MHC...
Natural killer (NK) cells express clonally distributed receptors for different groups of HLA class I alleles. The Z27 monoclonal antibody described in this study recognizes a p70 receptor specific HLA-B alleles belonging to the Bw4 supertypic specificity. Single amino acid substitutions peptide-binding groove HLA-B2705 molecules influenced recognition by some, but not all, p7O/Z27+ clones. This suggests existence limited polymorphism within p7O family receptors. pattern reactivity revealed...
Human herpesvirus 6 (HHV-6), a T-lymphotropic human herpesvirus, is potentially immunosuppressive agent that has been suggested to play role as cofactor in the natural history of immunodeficiency virus (HIV) infection. We studied interactions between HHV-6 and gamma/delta T lymphocytes, subset cells involved protective immune response against specific microorganisms. Polyclonal cell populations, purified from peripheral blood healthy adults activated vitro with phytohemagglutinin, were...