A5005987191- HIV Research and Treatment
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- Extracellular vesicles in disease
- Herpesvirus Infections and Treatments
- HIV/AIDS drug development and treatment
- Immunotherapy and Immune Responses
- HIV/AIDS Research and Interventions
- Atherosclerosis and Cardiovascular Diseases
- Monoclonal and Polyclonal Antibodies Research
- Reproductive tract infections research
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 and COVID-19 Research
- Reproductive System and Pregnancy
- CAR-T cell therapy research
- Mosquito-borne diseases and control
- Glycosylation and Glycoproteins Research
- Viral-associated cancers and disorders
- Cancer Immunotherapy and Biomarkers
- Mesenchymal stem cell research
- MXene and MAX Phase Materials
- Immunodeficiency and Autoimmune Disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Complement system in diseases
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2011-2024
Qatar Airways (Qatar)
2015-2024
Sidra Medical and Research Center
2015-2022
National Student Clearinghouse Research Center
2020
National Institutes of Health
2009-2018
Center for Translational Molecular Medicine
2017
Health and Human Development (2HD) Research Network
1998-2016
National Eye Institute
2016
Intercell (Austria)
2015
Eunice Kennedy Shriver Center
2015
The increase of proinflammatory cytokines in vaginal secretions may serve as a surrogate marker unwanted inflammatory reaction to microbicide products topically applied for the prevention sexually transmitted diseases, including HIV-1. Interleukin (IL)-1β and IL-6 have been proposed indicators inflammation increased risk HIV-1 transmission; however, lack information regarding detection platforms optimal fluids interlaboratory variation limit their use evaluation other clinical applications....
Objectives: Establishment of an in vitro model to evaluate rectal safety and the efficacy microbicide candidates. Design: An investigation characterization human colorectal explant culture for screening candidate microbicides prevent transmission HIV-1 infection. Methods: Human explants were cultured at liquid–air interface on gelfoam rafts. Phenotypic target cells was performed by fluorescence-activated cell sorter analysis. infection determined measurement p24 antigen release, viral RNA,...
To decipher the immunologic mechanisms of plaque maturation and rupture, it is necessary to analyze phenotypes distribution individual lymphocytes that migrate plaques, as well their activation at different stages formation.
While flow cytometry has been used to analyze the antigenic composition of individual cells, makeup viral particles is still characterized predominantly in bulk. Here, we describe a technology, "flow virometry," that can be for antigen detection on virions. The technology based binding magnetic nanoparticles virions, staining virions with monoclonal antibodies, separating formed complexes columns, and characterizing them cytometers. We this study distribution two antigens (HLA-DR LFA-1)...
Purpose: Extracellular vesicles (EV), such as exosomes, are important mediators of intercellular communication and have been implicated in modulation the immune system. We investigated if EV released from retinal pigment epithelium (RPE) modulate responses vitro. Methods: were isolated ARPE-19 cultures stimulated or not with inflammatory cytokines IL-1β, IFN-γ, TNF-α. Isolated characterized by nanoparticle flow Western blot analyses. Retinal epithelium–derived cultured human peripheral blood...
Although an association between human herpesvirus (HHV) infection and atherosclerosis has been suggested, the data supporting such are controversial and, in most cases, based on serological evidence or presence of cell-associated HHV DNA, which do not report about actual viral replication. We quantified DNA all 8 types HHVs plasma, their is replication.Using quantitative real-time polymerase chain reaction, we evaluated blood samples obtained at time hospitalization from 71 patients with...
The human chemokine receptors CCR5 and CXCR4 have emerged as the predominant cofactors, along with CD4, for cellular entry of HIV-1 in vivo whereas contribution other to HIV disease has not been yet determined. CCR5-specific (R5) viruses predominate during primary infection specificity (R5/X4 or X4 viruses) often emerge late stages disease. evolution is associated a rapid decline CD4+ T cells, although causative relationship between viral tropism cell depletion proven. To rigorously test...