A5000539432- Lipid Membrane Structure and Behavior
- Alzheimer's disease research and treatments
- Peroxisome Proliferator-Activated Receptors
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Protein Structure and Dynamics
- Plant tissue culture and regeneration
- Cholesterol and Lipid Metabolism
- Methane Hydrates and Related Phenomena
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- Plant Genetic and Mutation Studies
- Hydrocarbon exploration and reservoir analysis
- Seismic Imaging and Inversion Techniques
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Advanced Photonic Communication Systems
- Silicon and Solar Cell Technologies
- Viral gastroenteritis research and epidemiology
- Optical Network Technologies
- Protein Interaction Studies and Fluorescence Analysis
- Proteoglycans and glycosaminoglycans research
- Lipoproteins and Cardiovascular Health
- Mesoporous Materials and Catalysis
- Lipid metabolism and biosynthesis
- Advanced Optical Network Technologies
- Surfactants and Colloidal Systems
Kyoto Pharmaceutical University
2016-2025
Oki Electric Industry (Japan)
2012-2024
Tokyo Denki University
2015-2023
Nara Prefectural Institute of Public Health Research Center
2006-2022
Nihon University
1985-2021
Yokohama National University
1992-2021
Yamaha (Japan)
2020
Hirosaki University
2003-2019
Railway Technical Research Institute
2005-2019
NTT (Japan)
1992-2017
The B1 (CD20) molecule is a Mr 33,000 phosphoprotein on the surface of human B lymphocytes that may serve central role in humoral immune response by regulating B-cell proliferation and differentiation. In this report, cDNA clone encodes was isolated amino acid sequence determined. B-cell-specific clones were selected from tonsillar library differential hybridization with labeled derived either size-fractionated mRNA or T-cell mRNA. Of 261 isolated, 3 cross-hybridizing chosen as potential...
Heavy-ion mutagenesis is recognised as a powerful technology to generate new mutants, especially in higher plants. beams show high linear energy transfer (LET) and thus more effectively induce DNA double-strand breaks than other mutagenic techniques. Previously, we determined the most effective heavy-ion LET (LETmax: 30.0 keV μm(-1)) for Arabidopsis by analysing effect of on mutation induction. However, molecular structure mutated induced heavy ions with LETmax remains unclear. Knowledge...
Abstract Aggregation of α-synuclein (α-syn) into amyloid fibrils is closely associated with Parkinson’s disease (PD). Familial mutations or posttranslational truncations in α-syn are known as risk factor for PD. Here, we examined the effects PD-related A30P A53T point mutation and C-terminal 123–140 104–140 truncation on aggregating property based kinetic thermodynamic analyses. Thioflavin T fluorescence measurements indicated that A53T, Δ123‒140, Δ104–140 variants aggregated faster than WT...
Detailed structural information on human exchangeable apolipoproteins (apo) is required to understand their functions in lipid transport. Using a series of deletion mutants that progressively lacked different regions along the molecule, we probed organization lipid-free apoA-I and role domains binding, making comparisons apoE, which member same gene family known have two domains. Measurements α-helix content by CD conjunction with tryptophan 8-anilino-1-naphthalenesulfonic acid fluorescence...
Progression of skeletal muscle atrophy is one the characteristic features in cancer patients. Interleukin-6 (IL-6) has been reported to be responsible for loss lean body mass during cachexia colon-26 adenocarcinoma (C-26)-bearing mice. This study was carried out elucidate intracellular proteolytic pathways operating C-26-bearing mice, and examine effect anti IL-6 receptor antibody on atrophy. On day 17 after tumor inoculation, gastrocnemius weight mice had significantly decreased 69% that...
N-Acetylglucosaminyltransferase V (GnT-V) catalyzes the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to α-6-D-mannoside produce β1-6 linked branching N-glycan oligosaccharides, which controls polylactosamine content. The expression N-acetylglucosaminyltransferase V, contains 17 exons and spans 155 kilobase pairs, is expressed in a tissue- cell type-specific manner regulated at level transcription by multiple promoters (Saito, H., Gu, J., Nishikawa, A., Ihara, Y., Fujii,...
The einkorn wheat (Triticum monococcum) mutant, maintained vegetative phase (mvp), was induced by nitrogen ion-beam treatment and identified its inability to transit from the reproductive phase. In our previous study, we showed that WAP1 (wheat APETALA1) is a key gene in regulatory pathway controls transition growth common wheat. an ortholog of VRN1 responsible for vernalization insensitivity mvp mutation resulted deletion coding promoter regions, demonstrating WAP1/VRN1 indispensable...
Monochromatic high-energy X-rays (37 keV) focused into a microbeam (3.8 × 1.3 μm2) with Kirkpatrick-Baez mirror allows us for the first time to reveal striking sub-cellular distribution and chemical form of cadmium in hyperaccumulating plant, Arabidopsis halleri ssp. gemmifera, by detecting Cd Kα line. Micro X-ray fluorescence analysis (μ-XRF) micro absorption near-edge structure (μ-XANES) were used nondestructive at cellular level plant order investigate accumulation mechanism. It was...
Anthracyclines have long been considered to be among the most active agents clinically available for treatment of breast cancer despite their toxicity. To improve pharmacological profiles, a new macromolecular prodrug, denoted NC‐6300, was synthesized. NC‐6300 comprises epirubicin covalently bound polyethylene‐glycol polyaspartate block copolymer through an acid‐labile hydrazone bond. The conjugate forms micellar structure spontaneously in aqueous media with diameter 60–70 nm. copolymers are...
Apolipoprotein (apo) A-I is thought to undergo a conformational change during lipid association that results in the transition of random coil α-helix. Using series deletion mutants lacking different regions along molecule, we examined contribution α-helix formation apoA-I binding egg phosphatidylcholine (PC) small unilamellar vesicles (SUV). Binding isotherms determined by gel filtration showed binds SUV with high affinity and deletions C-terminal region markedly decrease affinity. Circular...
Apolipoprotein (apo) E contains two structural domains, a 22-kDa (amino acids 1–191) N-terminal domain and 10-kDa 223–299) C-terminal domain. To better understand apoE-lipid interactions on lipoprotein surfaces, we determined the thermodynamic parameters for binding of apoE4 its 22- fragments to triolein-egg phosphatidylcholine emulsions using centrifugation assay titration calorimetry. In both large (120 nm) small (35 emulsion particles, affinities decreased in order fragment ≈ 34-kDa...
The human B1 (CD20) molecule is a differentiation Ag found only on the surface of B lymphocytes. This structurally unique phosphoprotein plays role in regulation cell proliferation and differentiation. In order to determine whether this structure also expressed by murine cells, cDNA clones that encode mouse equivalent were isolated. longest clone isolated, pmB1-1, contained 1.4-kb insert with an 873 base pair open reading frame encodes protein 32 kDa. predicted contains three hydrophobic...
Human apolipoprotein (apo) E4 binds preferentially to very low-density lipoproteins (VLDLs), whereas apoE3 high-density (HDLs), resulting in different plasma cholesterol levels for the two isoforms. To understand molecular basis this effect, we engineered isolated apoE N-terminal domain (residues 1-191) and C-terminal 192-299) together with a series of variants containing deletions assessed their lipid lipoprotein binding properties. Both isoforms can bind phospholipid (PL)-stabilized...
Abstract We determined the molecular mechanisms underlying apolipoprotein E (ApoE)‐isoform‐dependent lipid efflux from neurons and ApoE‐deficient astrocytes in culture. The ability of ApoE3 to induce was 2.5‐ 3.9‐fold greater than ApoE4. To explore contributions amino‐ carboxyl‐terminal tertiary structure domains ApoE cellular efflux, each domain studied separately. amino‐terminal fragment (22‐kDa‐ApoE3) induced 22‐kDa‐ApoE4, whereas common very low levels efflux. Addition segments...