A5063610618- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Hematological disorders and diagnostics
- Tissue Engineering and Regenerative Medicine
- Periodontal Regeneration and Treatments
- Adipose Tissue and Metabolism
- Lymphatic System and Diseases
- Diabetic Foot Ulcer Assessment and Management
- Neurological disorders and treatments
- Embedded Systems Design Techniques
- Cytomegalovirus and herpesvirus research
- Bone and Joint Diseases
- Cell Image Analysis Techniques
- Adipokines, Inflammation, and Metabolic Diseases
- Digital Imaging for Blood Diseases
- Immune cells in cancer
- Autophagy in Disease and Therapy
- Advanced Sensor and Energy Harvesting Materials
- Congenital heart defects research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Molecular Biology Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cardiovascular Disease and Adiposity
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cardiac Fibrosis and Remodeling
University of Lausanne
2019-2024
École Polytechnique Fédérale de Lausanne
2017-2023
Copenhagen University Hospital
2013-2016
Rigshospitalet
2013-2016
The utility of mesenchymal stromal cells (MSCs) in therapeutic applications for regenerative medicine has gained much attention. Clinical translation MSC-based approaches requires vitro culture-expansion to achieve a sufficient number cells. ideal cell culture medium should be devoid any animal derived components. We have evaluated whether human Platelet Lysate (hPL) could an attractive alternative supplements.MSCs from bone marrow (BMSCs) and adipose tissue-derived (ASCs) obtained three...
The bone marrow (BM) exists heterogeneously as hematopoietic/red or adipocytic/yellow depending on skeletal location, age, and physiological condition. Mouse models patients undergoing radio/chemotherapy suffering acute BM failure endure rapid adipocytic conversion of the microenvironment, so-called "red-to-yellow" transition. Following hematopoietic recovery, such upon transplantation, a "yellow-to-red" transition occurs functional hematopoiesis is restored. Gold Standards to estimate...
Abstract A novel type of injectable biomaterial with an elastic softening transition is described. The material enables in vivo shaping, followed by induction 3D stable vascularized tissue. synthesis the meta‐biomaterial instructed extensive numerical simulation as a suspension irregularly fragmented, highly porous sponge‐like microgels. irregular particle shape dramatically enhances yield strain for stability against deformation. Porosity particles, along friction between internal surfaces,...
Human mesenchymal stromal cells from the bone marrow (BMSCs) are widely used as experimental regenerative treatment of ischemic heart disease, and first clinical trials using adipose-derived (ASCs) currently being conducted. Regenerative mechanisms BMSCs ASCs manifold in vitro pretreatment with growth factors has been applied to potentially enhance these properties. When characterizing transcriptional activity cellular it is important consider effect factor on reference genes (RGs) for...
Stimulation of mesenchymal stromal cells and adipose tissue-derived (ASCs) with vascular endothelial growth factor (VEGF) has been used in multiple animal studies clinical trials for regenerative purposes. VEGF stimulation is believed to promote angiogenesis usually performed under serum deprivation. Potential molecular mechanisms are numerous the role contributing factors uncertain. The aim current study was investigate effect vitro deprivation on gene expression patterns ASCs.Gene...
Bone marrow adipocytes (BMAds) constitute the most abundant stromal component of adult human bone marrow. Two subtypes BMAds have been described, more labile regulated (rBMAds) and stable constitutive (cBMAds), which develop earlier in life are resilient to environmental metabolic disruptions. In vivo, rBMAds enriched saturated fatty acids, contain smaller lipid droplets (LDs) readily provide hematopoietic support than their cBMAd counterparts. Mouse models used for research, but isolation...
Abstract Background Adipose-derived stromal cells (ASCs) stimulated with vascular endothelial growth factor (VEGF) and serum-deprived, are applied in the first in-man double-blind placebo-controlled MyStromalCell Trial, as a novel therapeutic option for treatment of ischemic heart disease (IHD). This vitro study explored effect VEGF serum deprivation on differentiation capacity ASCs from healthy donors IHD patients. Methods rhVEGF A165 serum-deprived medium one to three weeks were compared...
The 3rd International Meeting on Bone Marrow Adiposity (BMA) was held at the Olympic Museum in Lausanne, Switzerland, August 31st and September 1st, 2017. This brief monograph summarizes scientific contents of meeting highlights birth Society (BMAS).
Although hematopoietic stem cell (HSC) transplantation can restore functional hematopoiesis upon immune or chemotherapy-induced bone marrow failure, complications often arise during recovery, leading to up 25% transplant-related mortality in treated patients. In homeostasis and regeneration, HSCs the give rise entirety of cellular blood components. One challenges studying is ability successfully mimic relationship between stroma progenitor cells (HSPCs). This study described protocols...
Abstract Bone marrow adipocytes (BMAds) constitute the most abundant stromal component of adult human bone marrow. Two subtypes BMAds have been described, more labile regulated (rBMAds) and stable constitutive (cBMAds), which develop earlier are resilient to environmental metabolic disruptions. In vivo , rBMAds enriched in saturated fatty acids, contain smaller lipid droplets (LDs) readily provide hematopoietic support than their cBMAd counterparts. Mouse models used for research, but...