A5054789000- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Tissue Engineering and Regenerative Medicine
- Angiogenesis and VEGF in Cancer
- Mesenchymal stem cell research
- Preterm Birth and Chorioamnionitis
- Cellular Mechanics and Interactions
- Osteoarthritis Treatment and Mechanisms
- Periodontal Regeneration and Treatments
- Bone fractures and treatments
- Neonatal Respiratory Health Research
- Congenital Anomalies and Fetal Surgery
- Assisted Reproductive Technology and Twin Pregnancy
- Connective tissue disorders research
- Cancer Cells and Metastasis
- Polymer Surface Interaction Studies
- Blood properties and coagulation
- Urological Disorders and Treatments
- Hydrogels: synthesis, properties, applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Axon Guidance and Neuronal Signaling
- Surgical Sutures and Adhesives
- Hematopoietic Stem Cell Transplantation
- Proteoglycans and glycosaminoglycans research
University Hospital of Zurich
2016-2025
University of Zurich
2016-2025
Swiss Integrative Center for Human Health
2011-2023
Center for Pediatric Endocrinology Zurich
2021-2022
École Polytechnique Fédérale de Lausanne
2006-2022
University Hospital of Geneva
2022
University of Geneva
2022
ETH Zurich
2015
University of Freiburg
2013
KU Leuven
2012
Local, controlled induction of angiogenesis remains a challenge that limits tissue engineering approaches to replace or restore diseased tissues. We present new class bioactive synthetic hydrogel matrices based on poly(ethylene glycol) (PEG) and peptides exploits the activity vascular endothelial growth factor (VEGF) alongside base matrix functionality for cellular ingrowth, is, cell adhesion by pendant RGD-containing provision cell-mediated remodeling cross-linking metalloproteinase...
Although vascular endothelial growth factor (VEGF) has been described as a potent angiogenic stimulus, its application in therapy remains difficult: blood vessels formed by exposure to VEGF tend be malformed and leaky. In nature, the principal form of possesses binding site for ECM components that maintain it immobilized state until released local cellular enzymatic activity. this study, we present an engineered variant VEGF, alpha2PI1-8-VEGF121, mimics concept matrix-binding cell-mediated...
We present polymeric hydrogel biomaterials that are biomimetic both in their synthesis and degradation. The design of oligopeptide building blocks with dual enzymatic responsiveness allows us to create polymer networks formed functionalized via reactions degradable other reactions, occurring under physiological conditions. activated transglutaminase enzyme factor XIIIa was utilized for site-specific coupling prototypical cell adhesion ligands simultaneous cross-linking from...
We present here the biological performance in supporting tissue regeneration of hybrid hydrogels consisting genetically engineered protein polymers that carry specific features natural extracellular matrix, cross-linked with reactive poly(ethylene glycol) (PEG). Specifically, contain cell adhesion motif RGD, which mediates integrin receptor binding, and degradation sites for plasmin matrix-metalloproteinases, both being proteases implicated matrix remodeling. Biochemical assays as well vitro...
Growth and differentiation of multicellular systems is orchestrated by spatially restricted gene expression programs in specialized subpopulations. The targeted manipulation such processes synthetic tools with high-spatiotemporal resolution could, therefore, enable a deepened understanding developmental open new opportunities tissue engineering. Here, we describe the first red/far-red light-triggered switch for mammalian cells achieving control time space. We show that system can reversibly...
Significance Inducing the growth of new blood vessels by specific factors is an attractive strategy to restore flow in ischemic tissues. Vascular endothelial factor (VEGF) master regulator angiogenesis, yet clinical trials VEGF gene delivery failed. Major challenges include need control tissue distribution dose and duration expression. Here, we developed a highly tunable fibrin-based platform precisely protein Optimized fibrin-bound ensured normal, stable, functional angiogenesis improved...
Abstract We report a microfluidic approach for one‐step fabrication of polyelectrolyte microcapsules in aqueous conditions. Using two immiscible polymer solutions, we generate transient water‐in‐water‐in‐water double emulsion droplets and use them as templates to fabricate microcapsules. The capsule shell is formed by the complexation oppositely charged polyelectrolytes at interface. find that attractive electrostatic interactions can significantly prolong release molecules. Moreover,...
Abstract The accurate study of cellular microenvironments is limited by the lack technologies that can manipulate cells in 3D at a sufficiently small length scale. ability to build and multicellular microscopic structures will facilitate more detailed understanding function fields such as developmental stem cell biology. We present holographic optical tweezers based technology accurately generate bespoke micro-architectures. Using embryonic cells, varying geometries were created stabilized...
Netrins, a family of laminin-related molecules, have been proposed to act as guidance cues either during nervous system development or the establishment vascular system. This was clearly demonstrated for netrin-1 via its interaction with receptors DCC and UNC5s. However, mainly based on shared homologies netrin-1, netrin-4 also play role in neuronal outgrowth developmental/pathological angiogenesis interactions receptors. Here, we present high-resolution structure netrin-4, which shows...
For creating functional tissue analogues in engineering, stem cells require very specific 3D microenvironments to thrive and mature. Demanding (stem) cell types that are used nowadays can find such an environment a heterogeneous protein mixture with the trade name Matrigel. Several variations of synthetic hydrogel platforms composed poly(ethylene glycol) (PEG), which spiked peptides, have been recently developed shown equivalence Matrigel for differentiation. Here clinically relevant...
Abstract The clinical standard therapy for large bone defects, typically addressed through autograft or allograft donor tissue, faces significant limitations. Tissue engineering offers a promising alternative strategy the regeneration of substantial lesions. In this study, we harnessed poly(ethylene glycol) (PEG)-based hydrogels, optimizing critical parameters including stiffness, incorporation arginine-glycine-aspartic acid (RGD) cell adhesion motifs, degradability, and release BMP2 to...
Abstract The treatment of large bone defects requires tissue substitutes. However, the lack accessible autologous bone, especially in newborns with spina bifida or cleft palate conditions, severely limits therapeutic options involving grafts. Here, an engineering approach to reconstruct is presented by combining human amniocentesis‐derived amniotic fluid progenitor cells (hAFCs) and a biomimetic, injectable, fully synthetic poly(ethylene glycol) hydrogel that crosslinked enzymatically...