A5078791008- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Axon Guidance and Neuronal Signaling
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Cellular Mechanics and Interactions
- Biochemical and Structural Characterization
- Wnt/β-catenin signaling in development and cancer
- Toxic Organic Pollutants Impact
- RNA Interference and Gene Delivery
- Protein Structure and Dynamics
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Lipid Membrane Structure and Behavior
- Amino Acid Enzymes and Metabolism
- Advanced biosensing and bioanalysis techniques
- Galectins and Cancer Biology
- Hippo pathway signaling and YAP/TAZ
- Erythrocyte Function and Pathophysiology
- Mitochondrial Function and Pathology
- Muscle Physiology and Disorders
- Microtubule and mitosis dynamics
- Proteoglycans and glycosaminoglycans research
University of Manitoba
2016-2025
Research Manitoba
2017-2021
Medicon Village
2020
University of British Columbia
2013
Martin Luther University Halle-Wittenberg
2011
University of Basel
2000-2006
University of Zurich
2004
Max Planck Institute of Biochemistry
1996-2000
Human telomerase RNA (hTR) contains several guanine tracts at its 5'-end that can form a G4-quadruplex structure. Previous evidence suggests within this region disrupts the formation of an important structure hTR known as P1 helix, critical element in defining template boundary for reverse transcription. associated with AU-rich (RHAU) is helicase has specificity DNA and G4-quadruplexes. Two recent studies identify specific interaction between RHAU. Herein, we confirm minimally interacting...
Collagen VI-related myopathies are disorders of connective tissue presenting with an overlap phenotype combining clinical involvement from the muscle and tissue. Not all patients displaying related phenotypes between have mutations in collagen VI. Here, we report a homozygous recessive loss function mutation de novo dominant XII (COL12A1) as underlying novel syndrome involving Two siblings for showed widespread joint hyperlaxity combined weakness precluding independent ambulation, while...
Netrins, a family of laminin-related molecules, have been proposed to act as guidance cues either during nervous system development or the establishment vascular system. This was clearly demonstrated for netrin-1 via its interaction with receptors DCC and UNC5s. However, mainly based on shared homologies netrin-1, netrin-4 also play role in neuronal outgrowth developmental/pathological angiogenesis interactions receptors. Here, we present high-resolution structure netrin-4, which shows...
Guanine quadruplexes (G4s) are an important structure of nucleic acids (DNA and RNA) with roles in several cellular processes. RNA G4s require specialized unwinding enzymes, which only two have been previously identified. We describe the results a simple specific mass spectrometry guided method used to screen HEK293T cell lysate for G4 binding proteins. From these results, we validated helicase protein DDX21. DDX21 is established helicase, but has not yet as protein. Through biochemical...
Abstract RNA Helicase associated with AU-rich element (RHAU) (DHX36) is a DEAH (Aspartic acid, Glumatic Acid, Alanine, Histidine)-box helicase that can bind and unwind G4-quadruplexes in DNA RNA. To detect novel targets of RHAU, we performed an co-immunoprecipitation screen identified the PITX1 messenger (mRNA) as specifically highly enriched. homeobox transcription factor roles both development cancer. Primary sequence analysis three probable quadruplexes within 3′-untranslated region mRNA....
Recombinant proteins are commonly expressed in eukaryotic expression systems to ensure the formation of disulfide bridges and proper glycosylation. Although many can be easily, some proteins, sub-domains, mutant protein versions cause problems. Here, we investigated levels recombinant extracellular, intracellular as well transmembrane tethered different polypeptides mammalian cell lines. Strikingly, fusion prokaryotic maltose-binding (MBP) generally enhanced production. MBP consistently...
The identification of four-stranded G-quadruplexes (G4s) has highlighted the fact that DNA additional spatial organisations at its disposal other than double-stranded helices. Recently, it became clear formation G4s is not limited to traditional G3+NL1G3+NL2G3+NL3G3+ sequence motif. Instead, G3 triplets can be interrupted by deoxythymidylate (DNA) or uridylate (RNA) where base forms a bulge loops out from G-quadruplex core. Here, we report first high-resolution X-ray structure unique...
Rationale There is a need for validated method to improve detection limits and simultaneously quantify polycyclic aromatic compounds (PACs, both parent alkylated homologues) in biota by gas chromatography/tandem mass spectrometry because of their environmental significance. The validation the was performed accordance Eurachem Guide Quality Analytical Chemistry. Methods Gas chromatography coupled with triple quadrupole spectrometer used multiple reaction monitoring (MRM) mode quantification....
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as a global threat to human health has highlighted the need for development novel therapies targeting current and emerging coronaviruses with pandemic potential. main protease (Mpro, also called 3CLpro) is validated drug target against been heavily studied since SARS-CoV-2 in late 2019. Here, we report biophysical enzymatic characterization native Mpro, then characterize steady-state kinetics several commonly used...