A5082491793- Axon Guidance and Neuronal Signaling
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Galectins and Cancer Biology
- Hippo pathway signaling and YAP/TAZ
- Caveolin-1 and cellular processes
- Glycosylation and Glycoproteins Research
- Cellular Mechanics and Interactions
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Plant Toxicity and Pharmacological Properties
- Virus-based gene therapy research
- Ubiquitin and proteasome pathways
- Wnt/β-catenin signaling in development and cancer
- Signaling Pathways in Disease
- Cancer Mechanisms and Therapy
- CRISPR and Genetic Engineering
- Genomics, phytochemicals, and oxidative stress
- Nuclear Receptors and Signaling
- Biochemical and Molecular Research
- Peptidase Inhibition and Analysis
- Endoplasmic Reticulum Stress and Disease
- ATP Synthase and ATPases Research
- Lymphatic System and Diseases
- Pharmacogenetics and Drug Metabolism
University of Copenhagen
2018-2024
University of Freiburg
2023
University of Cologne
2011-2017
University of Lisbon
2016
Netrins, a family of laminin-related molecules, have been proposed to act as guidance cues either during nervous system development or the establishment vascular system. This was clearly demonstrated for netrin-1 via its interaction with receptors DCC and UNC5s. However, mainly based on shared homologies netrin-1, netrin-4 also play role in neuronal outgrowth developmental/pathological angiogenesis interactions receptors. Here, we present high-resolution structure netrin-4, which shows...
Article13 May 2020Open Access Source DataTransparent process Mechanisms of site-specific dephosphorylation and kinase opposition imposed by PP2A regulatory subunits Thomas Kruse orcid.org/0000-0002-2619-7388 Novo Nordisk Foundation Center for Protein Research, Faculty Health Medical Sciences, University Copenhagen, Denmark Search more papers this author Sebastian Peter Gnosa Biotech Research Innovation Centre (BRIC), Isha Nasa Biochemistry Cell Biology, Geisel School Medicine at Dartmouth...
Recombinant proteins are commonly expressed in eukaryotic expression systems to ensure the formation of disulfide bridges and proper glycosylation. Although many can be easily, some proteins, sub-domains, mutant protein versions cause problems. Here, we investigated levels recombinant extracellular, intracellular as well transmembrane tethered different polypeptides mammalian cell lines. Strikingly, fusion prokaryotic maltose-binding (MBP) generally enhanced production. MBP consistently...
Abstract Netrin-1 is a bifunctional chemotropic guidance cue that plays key roles in diverse cellular processes including axon pathfinding, cell migration, adhesion, differentiation, and survival. Here, we present molecular understanding of netrin-1 mediated interactions with glycosaminoglycan chains heparan sulfate proteoglycans (HSPGs) short heparin oligosaccharides. Whereas HSPGs act as platform to co-localise close the surface, oligosaccharides have significant impact on highly dynamic...
Desmoplasia is a common feature of aggressive cancers, driven by complex interplay protein production and degradation. Basigin type 1 integral membrane receptor secreted in exosomes or released ectodomain shedding from the cell surface. Given that soluble basigin increased circulation patients with poor cancer prognosis, we explored putative role ADAM12-generated progression. We show recombinant binds β1 integrin stimulates gelatin degradation migration cells matrix metalloproteinase (MMP)-...
The production of recombinant proteins for functional and biophysical studies, especially in the field structural determination, still represents a challenge as high quality quantities are needed to adequately perform experiments. This is part solved by optimizing protein constructs expression conditions maximize yields regular flask systems. Still, work flow effort can be substantial with no guarantee obtain improvements. study presents combination workflows that used dramatically increase...
Netrins, a family of laminin-related molecules, have been proposed to act as guidance cues either during nervous system development or the establishment vascular system.This was clearly demonstrated for netrin-1 via its interaction with receptors DCC and UNC5.Due shared homologies netrin-1, netrin-4 also play role in neuronal outgrowth developmental/pathological angiogenesis interactions receptors.Here we present 3.1 Å structure netrin-4[1], which possesses unique features comparison...
Laminin is a heterotrimeric molecule composed of an alpha, beta and gamma chain.It interacts with various ligands at the extracellular matrix plays pivotal role in many biological processes.Netrin4, one four secreted netrins, specifically laminin gamma-1 chain (Schneiders et al. 2007).We recently highlighted significance this complex using ex vivo studies (Reuten 2016).Furthermore, we also solved structure netrin4 3.1 Å that allowed us to reason why our previously reported netrin1 (Grandin...