A5054171294- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
- Legionella and Acanthamoeba research
- DNA Repair Mechanisms
- Antibiotic Resistance in Bacteria
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Enzyme Structure and Function
- Antibiotics Pharmacokinetics and Efficacy
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Inflammasome and immune disorders
- RNA Research and Splicing
- Photoreceptor and optogenetics research
- Neutropenia and Cancer Infections
- Photosynthetic Processes and Mechanisms
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Heme Oxygenase-1 and Carbon Monoxide
- Nuclear Structure and Function
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Biochemical and Molecular Research
- Cellular transport and secretion
University of Hawaiʻi at Mānoa
2014-2024
Dartmouth College
2020-2024
University of New Hampshire
2017-2024
University of Technology Sydney
2022
University of California, Los Angeles
2021
Cotton (United States)
2020
Vinmec International Hospital
2020
USC Norris Comprehensive Cancer Center
2020
University of Maryland, College Park
2009-2019
Vietnam Academy of Science and Technology
2018
Significance The innate immune system provides a first line of defense against invading pathogens. inflammasome is an complex that activates inflammatory caspases upon infection, causing cell death and IL-1 cytokine release, which initiate gram-negative bacterial pathogens but also mediate septic shock. Many studies have been performed using cells from mice, mice humans differ in their complement caspases. Instead caspase-11, encode the putative orthologs caspase-4 caspase-5. Here, we show...
The unfolded protein response (UPR) adjusts the cell’s folding capacity in endoplasmic reticulum (ER) according to need. IRE1 is most conserved UPR sensor eukaryotic cells. It has remained controversial, however, whether mammalian and yeast use a common mechanism for ER stress sensing. Here, we show that similar yeast, human IRE1α’s ER-lumenal domain (hIRE1α LD) binds peptides with characteristic amino acid bias. Peptides proteins bind hIRE1α LD’s MHC-like groove induce allosteric changes...
Inflammasome activation is important for antimicrobial defense because it induces cell death and regulates the secretion of IL-1 family cytokines, which play a critical role in inflammatory responses. The inflammasome activates caspase-1 to process secrete IL-1β. However, mechanisms governing IL-1α release are less clear. Recently, non-canonical was described that caspase-11 mediates pyroptosis Caspase-11 response Gram-negative bacteria requires Toll-like receptor 4 (TLR4)...
Abstract Chromatophore organs in cephalopod skin are known to produce ultra-fast changes appearance for camouflage and communication. Light-scattering pigment granules within chromatocytes have been presumed be the sole source of coloration these complex organs. We report discovery structural emanating precise register with expanded pigmented chromatocytes. Concurrently, using an annotated squid chromatophore proteome together microscopy, we identify a likely biochemical component this...
Article13 May 2020Open Access Source DataTransparent process Mechanisms of site-specific dephosphorylation and kinase opposition imposed by PP2A regulatory subunits Thomas Kruse orcid.org/0000-0002-2619-7388 Novo Nordisk Foundation Center for Protein Research, Faculty Health Medical Sciences, University Copenhagen, Denmark Search more papers this author Sebastian Peter Gnosa Biotech Research Innovation Centre (BRIC), Isha Nasa Biochemistry Cell Biology, Geisel School Medicine at Dartmouth...
The innate immune system is critical for host defense against microbial pathogens, yet many pathogens express virulence factors that impair function. Here, we used the bacterial pathogen Legionella pneumophila to understand how successfully overcomes subversion mechanisms. L. replicates within macrophages by using a type IV secretion translocate effectors into cell cytosol. As consequence of effector delivery, protein synthesis blocked at several steps, including translation initiation and...
ABSTRACT Legionella pneumophila , an intracellular pathogen responsible for the severe pneumonia Legionnaires' disease, uses its dot/icm -encoded type IV secretion system (T4SS) to translocate effector proteins that promote survival and replication into host cell cytosol. However, by introducing bacterial products cytosol, L. also activates cytosolic immunosurveillance pathways, thereby triggering robust proinflammatory responses mediate control of infection. Thus, pulmonary types infects...
Significance Calcium is an important intracellular second messenger that regulates many biological processes. Many extracellular environmental cues lead to cellular calcium-level changes, which impact on the output of gene expression. In cardiomyocytes, calcium known control expression at level transcription, whereas its role in regulating alternative splicing has not been explored. Our studies demonstrate these cells a network alternatively spliced exons exists, responds altered levels by...
The intricate network of protein-chaperone interactions is crucial for maintaining cellular function. Recent discoveries have unveiled the existence specialized chaperone assemblies, known as epichaperomes, which serve scaffolding platforms that orchestrate reconfiguration protein-protein interaction networks, thereby enhancing adaptability and proliferation. This study explores structural regulatory aspects with a particular focus on role post-translational modifications (PTMs) in their...
Phosphoprotein phosphatases (PPPs) regulate major signaling pathways, but the determinants of phosphatase specificity are poorly understood. This is because methods to investigate this at scale lacking. Here, we develop a novel in vitro assay, MRBLE:Dephos, that allows multiplexing dephosphorylation reactions determine preferences. Using establish amino acid preferences residues surrounding site for PP1 and PP2A-B55, which reveals common unique To compare MRBLE:Dephos results cellular...
Alzheimer9s disease (AD) is the leading cause of cognitive loss and neurodegeneration in developed world. Although its genetic environmental causes are not generally known, familial forms (FAD) attributable to mutations a single copy Presenilin (<i>PS</i>) amyloid precursor protein genes. The dominant inheritance pattern FAD indicates that it may be gain or change function mutations. Studies FAD-linked presenilin (<i>psn</i>) model organisms, however, indicate they function, possibility...
Coxiella burnetii replicates within permissive host cells by employing a Dot/Icm type IV secretion system (T4SS) to translocate effector proteins that direct the formation of parasitophorous vacuole. C57BL/6 mouse macrophages restrict intracellular replication C. burnetii. Nine Mile phase II (NMII) strain. However, eliminating Toll-like receptor 2 (TLR2) permits bacterial replication, indicating restriction is immune mediated. Here, we examined whether additional innate pathways are employed...
Autism spectrum disorder (ASD) is a complex with an unclear aetiology and estimated global prevalence of 1%. However, studies ASD in the Vietnamese population are limited. Here, we first conducted whole exome sequencing (WES) 100 children their unaffected parents. Our stringent analysis pipeline was able to detect 18 unique variants (8 de novo 10 ×-linked, all validated), including 12 newly discovered variants. Interestingly, notable number X-linked were detected (56%), them found affected...
Meiosis is a specialized cell cycle that requires sequential changes to the division machinery facilitate changing functions. To define mechanisms enable oocyte-to-embryo transition, we performed time-course proteomics in synchronized sea star oocytes from prophase I through first embryonic cleavage. Although found protein levels were broadly stable, our analysis reveals dynamic waves of phosphorylation underlie each meiotic stage. We phosphatase PP2A-B55 reactivated at meiosis I/meiosis II...
MiRNAs are small non-coding RNAs that post-transcriptionally regulate gene expression in organisms ranging from viruses to mammals. There is great relevance understanding how miRNAs genes involved the growth, development, and maturation of many parasitic worms (helminths) together afflict more than 2 billion people.Here, we describe expressed by each predominant intra-mammalian development stages Fasciola hepatica, a foodborne flatworm infects wide range mammals worldwide, most importantly...
EXOC5 is a crucial component of large multi-subunit tethering complex, the exocyst that required for fusion secretory vesicles with plasma membrane. Exoc5 deleted mice die as early embryos. Therefore, to determine role in follicular and oocyte development, it was necessary produce conditional knockout (cKO), Zp3-Exoc5-cKO, which only oocytes. The first wave folliculogenesis appeared histologically normal progressed antral stage. However, after IVF sperm, oocytes collected from (superovulated...
Phosphoprotein phosphatases (PPPs) are the key serine/threonine that regulate all essential signaling cascades. In particular, Protein Phosphatase 1 (PP1) dephosphorylates ~80% of ser/thr phosphorylation sites. Here, we developed a phosphatase targeting peptide (PhosTAP) binds PP1 isoforms and does so with stronger affinity than any other known regulator. This PhosTAP can be used as recruitment tool for Phosphorylation Targeting Chimera (PhosTAC)-type in vitro cellular experiments, well...