A5042119593- Plant Stress Responses and Tolerance
- RNA Research and Splicing
- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- RNA modifications and cancer
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Plant Micronutrient Interactions and Effects
- Plant and Fungal Interactions Research
- Genomics and Phylogenetic Studies
- Plant tissue culture and regeneration
- Neurofibromatosis and Schwannoma Cases
- Wheat and Barley Genetics and Pathology
- Neurological Disorders and Treatments
- Aluminum toxicity and tolerance in plants and animals
- Plant responses to water stress
- Hippo pathway signaling and YAP/TAZ
- Legume Nitrogen Fixing Symbiosis
- RNA Interference and Gene Delivery
- Selenium in Biological Systems
- Redox biology and oxidative stress
- ATP Synthase and ATPases Research
- Biochemical and Structural Characterization
Panjab University
2019-2024
Ministry of AYUSH
2024
The University of Texas Health Science Center at Houston
2016
Case Western Reserve University
2011-2015
Institut thématique Génétique, génomique et bioinformatique
2014
Wright State University
2006-2011
Subnuclear organization and spatiotemporal regulation of pre-mRNA processing factors is essential for the production mature protein-coding mRNAs. We have discovered that a large protein called Son has novel role in maintaining proper nuclear speckles. The primary sequence contains concentrated region multiple unique tandem repeat motifs may support as scaffolding RNA used interference (RNAi) approaches high-resolution microscopy techniques to study functions context intact cells. Although...
Thaumatin-like proteins (TLPs) are related to pathogenesis-related-5 (PR-5) family and involved in stress response. Herein, a total of 93 TLP genes were identified the genome Triticum aestivum. Further, we 26, 27, 39, 37 Brachypodium distachyon, Oryza sativa, Sorghum bicolor, Zea mays genomes for comparative characterization, respectively. They could be grouped into small long TLPs with conserved thaumatin signature motif. Tightly clustered exhibited gene protein structure. The...
Significance Calcium is an important intracellular second messenger that regulates many biological processes. Many extracellular environmental cues lead to cellular calcium-level changes, which impact on the output of gene expression. In cardiomyocytes, calcium known control expression at level transcription, whereas its role in regulating alternative splicing has not been explored. Our studies demonstrate these cells a network alternatively spliced exons exists, responds altered levels by...
The hyperosmolality-gated calcium-permeable channels (OSCA) are pore-forming transmembrane proteins that function as osmosensors during various plant developmental processes and stress responses. In our analysis, through in silico approaches, a total of 42 OSCA genes identified the Triticum aestivum genome. A phylogenetic analysis reveals close clustering Arabidopsis thaliana, Oryza sativa, T. all clades, suggesting their origin before divergence dicots monocots. Furthermore, evolutionary...
Serine-arginine-rich (SR) proteins play a key role in alternative pre-mRNA splicing eukaryotes. We recently showed that large SR protein called Son has unique repeat motifs are essential for maintaining the subnuclear organization of processing factors nuclear speckles. Motif analysis highlights putative RNA interaction domains suggest direct splicing. Here, we used situ approaches to show localizes reporter minigene transcription site, and RNAi-mediated depletion causes exon skipping on...
Two-pore K+ (TPK) channels are voltage-independent and involved in stress response plants. Herein, we identified 12 TaTPK genes located on nine chromosomes the Triticum aestivum genome. The majority of comprised two exons. Each channel four transmembrane (TM) helices, N- C-terminal ion-channel domains, EF-hand domains one 14-3-3 binding site. Additionally, highly conserved 'GYGD' motif responsible for ion specificity, was found between TMs both domains. Nine were predicted to be localised at...
WUSCHEL-related homeobox (WOX) genes belong to the superfamily, are plant-specific and play vital functions in growth development. Herein, we identified a total of 43 TaWOX allohexaploid (AABBDD) genome Triticum aestivum L. These were distributed on various chromosomes each subgenome (A, B D). The phylogenetic analysis showed clustering TaWOXs into three clades: ancient, intermediate modern or WUS. gene protein structures including exon/intron organization, intron phases, domain motif...
Piezo channels belong to an important class of cell membrane-bound, Ca2+-permeable, mechanosensitive consisting a pore and multiple transmembrane helices. In plants, the functional aspects have been less studied than other channels. However, few studies that carried out indicate involvement in stress response developmental processes. our analysis, we identified total three genes Triticum aestivum genome. The phylogenetic analysis revealed monocot dicot-specific clustering proteins. gene...
In plants, Ca2+ is an integral element in several stresses related signallings. During environmental stresses, the cytosolic concentration tends to rise rapidly, which further co-ordinate with EF-hand domain-containing proteins. our study, a total of 586 proteins were identified Triticum aestivum as calmodulin (CaM)/Calmodulin-like (CML), calcineurin B-like (CBL), protein kinase (CDPK, CRK, CCaMK), respiratory burst oxidase homologs (RBOHs), miscellaneous and un-categorized (UEPs). These...
Boron (B) is an essential micronutrient of plants. Plants grapple with a narrow range B between its toxicity and deficiency. homeostasis mechanism required to rescue plants from such quagmire. transporters are specialized proteins involved in the B. In present study, total 29 BOR genes were identified five major cereals, including three BORs each Brachypodium distachyon Sorghum bicolor, four Oryza sativa, six Zea mays, 13 Triticum aestivum. Multiple sequence alignments, domain structure...
Neurofibromatosis type I (Nf1) is a GTPase-activating protein (GAP) that inactivates the oncoprotein Ras and plays important roles in nervous system development learning. Alternative exon 23a falls within Nf1 GAP domain coding sequence tightly regulated favor of skipping neurons; however, its biological function not fully understood. Here we generated mouse embryonic stem (ES) cells with constitutive endogenous inclusion, termed 23aIN/23aIN cells, by mutating splicing signals surrounding to...
Appropriate activation of the Ras/extracellular signal-regulated kinase (ERK) protein signaling cascade within brain is crucial for optimal learning and memory. One key regulator this Nf1 Ras GTPase activating (RasGAP), which attenuates Ras/ERK by converting active bound to guanosine triphosphate, into inactive diphosphate, inactivating Ras. A previous study using embryonic stem cells cell-derived neurons indicated that RasGAP activity modulated highly regulated alternative splicing exon...
Alternative splicing in eukaryotes plays an important role regulating gene expression by selectively including alternative exons. A wealth of information has been accumulated that explains how exons are selected a developmental stage- or tissue-specific fashion. However, our knowledge cells respond to environmental changes alter is very limited. For example, although number have shown be regulated calcium level alterations, the underlying mechanisms not well understood. As signaling neurons...
The four Hu [embryonic lethal abnormal vision-like (ELAVL)] protein family members regulate alternative splicing by binding to U-rich sequences surrounding target exons and affecting the interaction of machinery and/or local chromatin modifications. Each proteins contains a divergent N-terminus, three highly conserved RNA recognition motifs (RRM1, RRM2 RRM3) hinge region separating RRM3. roles each domain in regulation are not well understood. Here, we investigate how HuC, relatively poorly...
Objective Frontiers between pediatric bipolar disorder (PBD) and attention‐deficit/hyperactivity (ADHD) are not well defined. Few studies have addressed potentially different neurobiological factors the two disorders. Brain‐derived neurotrophic factor (BDNF) has been increasingly recognized for its etiologic prognostic role in adult (BD) studies. This study aimed to examine BDNF gene polymorphism potential alterations serum levels ADHD patients with or without comorbid BD illness. Method We...