A5036188178- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Molecular Biology Techniques and Applications
- melanin and skin pigmentation
- Skin Protection and Aging
- MicroRNA in disease regulation
- Vitamin D Research Studies
- RNA modifications and cancer
- Cell death mechanisms and regulation
- Mechanisms of cancer metastasis
- RNA Research and Splicing
- Retinoids in leukemia and cellular processes
- PI3K/AKT/mTOR signaling in cancer
- Epigenetics and DNA Methylation
- Melanoma and MAPK Pathways
- Microtubule and mitosis dynamics
- Esophageal Cancer Research and Treatment
- Cancer and Skin Lesions
- Polyamine Metabolism and Applications
- Exercise and Physiological Responses
- Advanced biosensing and bioanalysis techniques
- Cancer Cells and Metastasis
- Total Knee Arthroplasty Outcomes
- Ubiquitin and proteasome pathways
- Silk-based biomaterials and applications
Wright State University
2014-2024
Boonton Public Schools
2013-2016
Dayton Clinical Oncology Program
2013
Nanjing University
2012
Yangzhou University
2012
University of Cincinnati
2012
Brigham and Women's Hospital
2007
Wright-Patterson Air Force Base
2006
UPMC Hillman Cancer Center
1997-1998
Recent evidence suggests a major role of tumor-stromal interactions in pancreatic cancer pathobiology. The chemokine CXCL12 (stromal cell-derived factor 1 (SDF-1)), abundantly produced by stromal cells, promotes progression, metastasis, and chemoresistance cells. On the other hand, tumor sonic hedgehog (SHH) acts predominantly on cells to induce desmoplasia and, thus, has paracrine effect tumorigenesis therapeutic outcome. In this study, we examined association between these two proteins...
Epidermal melanocytes are skin cells specialized in melanin production. Activation of the melanocortin 1 receptor (MC1R) on by α-melanocyte-stimulating hormone (α-MSH) induces synthesis brown/black pigment eumelanin that confers photoprotection from solar UV radiation (UVR). Contrary to keratinocytes, slow proliferating persist for decades, an environment with high levels UVR-induced reactive oxygen species (ROS). We previously reported addition its role pigmentation, α-MSH also reduces...
In a randomized, dose-response trial, we used molecular and phenomic profiling to compare responses traditional (TRAD) endurance plus resistance training high-intensity tactical (HITT). Ninety-four participants (18-27 years) completed 12 weeks of TRAD or HITT followed by 4 detraining. While in vivo phenotype improvements were not dose-dependent, few dose-dependent ex muscle adaptations overshadowed wide-ranging inter-individual response heterogeneity (IRH). To address this, established...
Silk is an attractive biomaterial for use in tissue engineering applications because of its slow degradation, excellent mechanical properties, and biocompatibility. In this report, we demonstrate a simple method to cast patterned films directly from silk fibroin dissolved ionic liquid. The the liquid solution were found support normal cell proliferation differentiation. versatility solutions ability process large amounts into materials with controlled surface topography could enhance...
Inactivation of the Pten tumor suppressor negatively regulates PI3K-mTOR pathway. In a model cutaneous squamous cell carcinoma (SCC), we demonstrate that deletion strongly elevates Fgf10 protein levels without increasing transcription in vitro and vivo. The translational activation by is reversed genetic disruption mTORC1 complex, which also prevents skin tumorigenesis mutants. We further show ectopic expression causes papillomas, whereas deletion-induced tumors are inhibited epidermal...
Peptide-mediated internalization and organelle targeting of quantum dots.
Wound healing is a complex, multistep process that can be summarized into three stages, namely, hemostasis and inflammation, proliferation, finally, tissue remodeling. Battlefield wound demands rapid using clotting or cauterizing agents to immediately limit blood loss, but this occurs at the expense of proper repair beyond hemostasis. Layered silicate clays such as kaolin montmorillonite (MMT) have been previously shown induce due their ability form charged interactions with factors. The...
Serine-arginine-rich (SR) proteins play a key role in alternative pre-mRNA splicing eukaryotes. We recently showed that large SR protein called Son has unique repeat motifs are essential for maintaining the subnuclear organization of processing factors nuclear speckles. Motif analysis highlights putative RNA interaction domains suggest direct splicing. Here, we used situ approaches to show localizes reporter minigene transcription site, and RNAi-mediated depletion causes exon skipping on...
p63, a p53-related protein, has been shown to activate p53-responsive genes and induce apoptosis in certain cell types. In this study, we examined the effects of Mdm2 MdmX proteins on p63 transactivation, apoptosis, protein levels. The isoforms most structurally similar p53, p63γ (p51A) p63α (p51B), were chosen for study. Our results confirm earlier reports demonstrating that although both can transactivate promoters stronger transactivation potential is more potent inducer than p63α....
Abstract p63 and p73 are members of the p53 protein family have been shown to play an important role in cell death, development, tumorigenesis. In particular, has be involved maintenance epidermal stem cells stratification epidermis. Sonic Hedgehog (Shh) is a morphogen that also implicated epithelial proliferation development organs. Recently, Shh progression variety cancers. this report, we show but not overexpression induces expression. p63γ p63β (both TA ΔN isoforms) TAp73β isoform induce...
Loss of the tumor suppressor PTEN is observed in many human cancers that display increased chromosome instability and aneuploidy. The subcellular fractions are associated with different functions regulate cell growth, invasion stability. In this study, we show a novel role for regulating mitotic centrosomes. localization at centrosomes peaks between prophase metaphase, paralleling centrosomal PLK-1 γ-tubulin coinciding time frame centrosome maturation. primary keratinocytes, knockdown...
Barrett's esophagus (BE) is the precursor lesion and a major risk factor for esophageal adenocarcinoma (EAC). Although patients with BE undergo routine endoscopic surveillance, current screening methodologies have proven ineffective at identifying individuals of EAC. Since microRNAs (miRNAs) potential diagnostic prognostic value as disease biomarkers, we sought to identify an miRNA signature EAC.High-throughput sequencing miRNAs was performed on serum tissue biopsies from 31 identified...
The p63 transcription factor has a pivotal role in epithelial morphogenesis. Multiple transcripts of the TP63 gene are generated because alternative promoter usage and splicing. ΔNp63α is predominant isoform observed during morphogenesis human cancers. Loss expression been shown to promote invasiveness subset cancer cell lines. Here, we studied whether regulation VDR by controls an epidermoid line. We demonstrate that induced all isoforms, including ΔNp63α. Endogenous protein was bind...
1α, 25-dihydroxyvitamin D3 (VD3), a secosteriod that has been explored as an anti-cancer agent, was also shown to promote cell survival. Its receptor, the Vitamin D Receptor (VDR), is direct target of proto-oncogene ΔNp63α, which overexpressed in non-melanoma skin cancers. The interconnection between VDR/VD3 signaling and led us examine whether promotes keratinocyte proliferation by regulating ΔNp63α levels. Our data demonstrate VDR regulates expression at both transcript protein level....
Transmission electron microscopy (TEM) is being pushed to new capabilities which enable studies on systems that were previously out of reach. Among recent innovations, TEM through liquid cells (LC-TEM) enables in operando observation biological phenomena. This work applies LC-TEM the study components as they interact an abiotic surface. Specifically, analytes or target molecules like neuropeptide Y (NPY) are observed functional graphene field-effect transistor (GFET) biosensors. Biological...
The p53 family member, p73, has been characterized as a tumor suppressor and functions in similar manner to induce cellular death. phosphatase tensin homolog (PTEN) can function dual specificity lipid/protein phosphatase. However, recent data have described multiple roles for nuclear PTEN independent of its lipid activity. directly or indirectly activate promote apoptosis. We examined whether would interact regulate p73 p53. Co-localization the nucleus complex formation p73/PTEN were...