A5018147607- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Hematopoietic Stem Cell Transplantation
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Cellular Mechanics and Interactions
- Cystic Fibrosis Research Advances
- Hematological disorders and diagnostics
- Digital Imaging for Blood Diseases
- Retinal Development and Disorders
- Caveolin-1 and cellular processes
- Planarian Biology and Electrostimulation
- Erythrocyte Function and Pathophysiology
- Neonatal Respiratory Health Research
- Biomedical Ethics and Regulation
- Single-cell and spatial transcriptomics
- Barrier Structure and Function Studies
- Silk-based biomaterials and applications
- Neuroscience and Neural Engineering
- Pharmacogenetics and Drug Metabolism
- Cancer Research and Treatments
- Renal and related cancers
- Sex and Gender in Healthcare
- Cellular transport and secretion
École Polytechnique Fédérale de Lausanne
2011-2024
University Hospital of Geneva
2022
University Hospital of Zurich
2022
University of Geneva
2022
Bioengineering (Switzerland)
2016
University of Lausanne
2015
Bioengineering Center
2015
Abstract The development of improved methods to culture retinal organoids is relevant for the investigation mechanisms under pathophysiological conditions, screening neuroprotective compounds, and providing a cellular source clinical transplantation. We report tissue-engineering approach accelerate standardize production by culturing mouse embryonic stem cells (mESC) in optimal physico-chemical microenvironments. Arrayed round-bottom milliwells composed biomimetic hydrogels, combined with an...
The treatment of bone defects with recombinant morphogenetic protein-2 (BMP-2) requires high doses precluding broad clinical application. Here, a bioengineering approach is presented that strongly improves low-dose BMP-2-based regeneration by mobilizing healing-associated mesenchymal progenitor cells (MPCs). Smart synthetic hydrogels are used to trap and study endogenous MPCs trafficking defects. Hydrogel-trapped prospectively isolated differentiate into multiple lineages in vitro form vivo....
The difficulty of studying post-implantation development in mammals has sparked a flurry activity to develop vitro models, termed embryoids, based on self-organizing pluripotent stem cells. Previous approaches derive embryoids either lack the physiological morphology and signaling interactions, or are unconducive model post-gastrulation development. Here, we report bioengineering-inspired approach aimed at addressing this gap. We employ high-throughput cell aggregation simultaneously coax...
Abstract Background The pathways that control protein transport across the blood–brain barrier (BBB) remain poorly characterized. Despite great advances in recapitulating human BBB vitro, current models are not suitable for systematic analysis of molecular mechanisms antibody transport. gaps our mechanistic understanding transcytosis hinder new therapeutic delivery strategy development. Methods We applied a novel bioengineering approach to generate organoids by self-assembly astrocytes,...
In many leukemia patients, a poor prognosis is attributed either to the development of chemotherapy resistance by leukemic stem cells (LSCs) or inefficient engraftment transplanted hematopoietic stem/progenitor (HSPCs) into bone marrow (BM). Here, we build 3D in vitro model system organoids (BMOs) that recapitulate several structural and cellular components native BM. These are formed high-throughput manner from aggregation endothelial mesenchymal within hydrogel microwells. Accordingly,...
Fate choices of stem cells are regulated in response to a complex array biochemical and physical signals from their microenvironmental niche. Whereas the molecular composition role mechanical niche cues have been extensively studied, relatively little is known about how both effectors act concert modulate cell fate. Here we utilized recently developed artificial microarray platform investigate whether stiffness culture substrate influences signaling factors exert on adipogenic...
Objectives: Developing ex vivo models that replicate immune-tumor interactions with high fidelity is essential for advancing immunotherapy research, as traditional two-dimensional in vitro systems often lack the complexity required to fully represent these interactions. Methods: In this study, we establish a comprehensive 3D redirect lysis (3D-RDL) assay using colorectal cancer spheroids and adult stem cell-derived, healthy human organoids evaluate efficacy safety profile of Cibisatamab,...
High failure rates of the current drug development process are driving exemplary changes toward methodologies centered on human disease
Juxtacrine or contact-dependent signaling is a major form of cell communication in multicellular organisms. The involved cell–cell and cell–extracellular-matrix (ECM) interactions are crucial for the organization maintenance tissue architecture function. However, because contacts relatively weak, it difficult to isolate interacting cells their native state study, example, how specific types interact with others (e.g., stem niche cells) where they locate within tissues execute tasks. To...
The difficulty of studying post-implantation development in mammals has sparked a flurry activity to develop vitro models, termed embryoids, based on self-organizing pluripotent stem cells. Previous approaches derive embryoids either lack the physiological morphology and signaling interactions, or are not yet optimal for modeling post-gastrulation development. Here, we report bioengineering-inspired approach aimed at addressing this gap. A high-throughput cell aggregation was employed...
Abstract Patient-derived organoids (PDOs) are the reference in vitro human disease models. However, utility of colorectal cancer (CRC) PDOs is hindered by lack a tumor microenvironment (TME). To address this limitation, we built living biobank CRC with autologous stromal and immune TME. We characterized original tumors traditional monocultures using single-cell RNA-seq (scRNA-seq) whole exome sequencing (WES) to obtain insights into cell type selection phenotypic drift culture. Subsequently,...
Abstract Background The pathways that control protein transport across the Blood-Brain Barrier (BBB) remain poorly characterized. Despite great advances in recapitulating human BBB vitro , current models are not suitable for systematic analysis of molecular mechanisms antibody transport. gaps our mechanistic understanding transcytosis hinder new therapeutic delivery strategy development. Methods We applied a novel bioengineering approach to generate organoids by self-assembly astrocytes,...
In many leukemia patients, a poor prognosis is attributed either to the development of chemotherapy resistance by leukemic stem cells (LSCs) or inefficient engraftment transplanted hematopoietic stem/progenitor (HSPCs) into bone marrow (BM). Here, we build 3D in vitro model system organoids (BMOs) that recapitulate several structural and cellular components native BM. These are formed high-throughput manner from aggregation endothelial mesenchymal within hydrogel microwells. Accordingly,...