A5043552394- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Molecular Biology Techniques and Applications
- Animal Genetics and Reproduction
- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Erythrocyte Function and Pathophysiology
- Antimicrobial Resistance in Staphylococcus
- Epigenetics and DNA Methylation
- Bacterial Identification and Susceptibility Testing
- 3D Printing in Biomedical Research
- RNA Research and Splicing
- Streptococcal Infections and Treatments
- Pancreatic function and diabetes
- Muscle Physiology and Disorders
- Reproductive Biology and Fertility
- Cardiomyopathy and Myosin Studies
- Bacterial biofilms and quorum sensing
- Stress Responses and Cortisol
- bioluminescence and chemiluminescence research
- Neonatal and fetal brain pathology
- Birth, Development, and Health
- Genomics and Chromatin Dynamics
ETH Zurich
2015-2023
Board of the Swiss Federal Institutes of Technology
2015-2023
Imperial College London
2018-2023
Hubrecht Institute for Developmental Biology and Stem Cell Research
2010-2015
University Medical Center Utrecht
2012-2015
Royal Netherlands Academy of Arts and Sciences
2012
Harvard University
2010
Massachusetts General Hospital
2010
Maastricht University
2006-2009
At the blastocyst stage of mammalian pre-implantation development, three distinct cell lineages have formed: trophectoderm, hypoblast (primitive endoderm) and epiblast. The inability to derive embryonic stem (ES) lines in a variety species suggests divergence between signaling pathways involved early lineage specification. In mouse, segregation primitive endoderm from pluripotent epiblast depends on FGF/MAP kinase signaling, but it is unknown whether this conserved species. Here we examined...
The scarcity of primordial germ cells (PGCs) in the developing mammalian embryo hampers robust biochemical analysis processes that underlie early cell formation. Here, we demonstrate DAZL, a cell-specific RNA binding protein, is PGC marker during vitro development. Using Dazl-GFP reporter ESCs, DAZL plays central role large mRNA/protein interactive network blocks translation core pluripotency factors, including Sox2 and Sall4, as well Suz12, polycomb family member required for...
In recent years, the perception of Z-disc function has changed from a passive anchor for myofilaments that allows transmission force, to dynamic multicomplex structure, capable sensing and transducing extracellular signals. Here, we describe new protein, which named CHAP (cytoskeletal heart-enriched actin-associated protein), expressed in differentiating heart skeletal muscle vitro vivo. Interestingly, addition its sarcomeric localization, was also able translocate nucleus. associated with...
Mechanosensing is a ubiquitous process to translate external mechanical stimuli into biological responses. Piezo1 ion channels are directly gated by forces and play an essential role in cellular mechanotransduction. However, readouts of activity mainly examined invasive or indirect techniques, such as electrophysiological analyses cytosolic calcium imaging. Here, we introduce GenEPi, genetically-encoded fluorescent reporter for non-invasive optical monitoring Piezo1-dependent activity. We...
Recent data demonstrates that stem cells can exist in two morphologically, molecularly and functionally distinct pluripotent states; a naïve LIF-dependent state which is represented by murine embryonic (mESCs) an FGF-dependent primed rat epiblast (EpiSCs). We find derivation of induced (iPSCs) under EpiSC culture conditions yields iPSCs from hereon called FGF-iPSCs) which, unexpectedly, display ES-like/ICM properties. FGF-iPSCs X-chromosome activation, multi-lineage differentiation, teratoma...
Accurate lineage reconstruction of mammalian pre-implantation development is essential for inferring the earliest cell fate decisions. Lineage tracing using global fluorescence labeling techniques complicated by increasing density and rapid embryo rotation, which hampers automatic alignment accurate tracking obtained four-dimensional imaging data sets. Here, we exploit advantageous properties primed convertible fluorescent proteins (pr-pcFPs) to simultaneously visualize green photoconverted...
Abstract Mechanosensing is a ubiquitous process to translate external mechanical stimuli into biological responses during development, homeostasis, and disease. However, non-invasive investigation of cellular mechanosensing in complex intact live tissue remains challenging. Here, we developed GenEPi, genetically-encoded fluorescent intensiometric reporter for based on Piezo1, an essential mechanosensitive ion channel found vertebrates. We show that GenEPi has high specificity spatiotemporal...
In recent years, advances in imaging probes, cutting-edge microscopy techniques and powerful bioinformatics image analysis have markedly expanded the toolbox available to developmental biologists. Apart from traditional qualitative studies, embryonic development can now be investigated vivo with improved spatiotemporal resolution, more detailed quantitative analyses down single-cell level of developing embryo. Such tools provide many benefits investigate emergence asymmetry early mammalian...
Mammalian embryonic development starts with a single fertilized zygote that develops into blastocyst embryo consisting of three cell types evolve either or extra-embryonic tissues. Lineage tracing these cells can provide important information about the molecular and cellular dynamics contributing to fate allocation during early development. While global labeling techniques allow for visualization all at same time, lineage over several divisions become complicated due movement rotation as...
Abstract Accurate lineage reconstruction of mammalian pre-implantation development is essential for inferring the earliest cell fate decisions development. Lineage tracing using global labeling techniques complicated by increasing density and rapid embryo rotation, impeding automatic alignment rendering accurate tracking obtained four-dimensional imaging data sets highly challenging. Here, we exploit advantageous properties primed convertible fluorescent proteins (pr-pcFPs) to simultaneously...