A5085237343- Force Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- Cellular Mechanics and Interactions
- Mechanical and Optical Resonators
- Photoreceptor and optogenetics research
- Cell Adhesion Molecules Research
- ATP Synthase and ATPases Research
- Molecular Junctions and Nanostructures
- Protein purification and stability
- Bacterial Genetics and Biotechnology
- Advanced Electron Microscopy Techniques and Applications
- Microtubule and mitosis dynamics
- Polymer Surface Interaction Studies
- Receptor Mechanisms and Signaling
- Glycosylation and Glycoproteins Research
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Linguistic Education and Pedagogy
- Protein Structure and Dynamics
- Microfluidic and Bio-sensing Technologies
- RNA and protein synthesis mechanisms
- Neuroscience and Neuropharmacology Research
- Linguistic research and analysis
- Monoclonal and Polyclonal Antibodies Research
- Alzheimer's disease research and treatments
ETH Zurich
2016-2025
University of Southampton
2023-2025
Cancer Research UK
2025
Board of the Swiss Federal Institutes of Technology
2015-2024
Southampton General Hospital
2023
Daimler (Germany)
2022
Rheinland-Pfälzische Technische Universität Kaiserslautern-Landau
2022
Reaction Biology (Germany)
2022
University of Zurich
2022
University of Kaiserslautern
2022
Article14 July 2016Open Access Source DataTransparent process GSDMD membrane pore formation constitutes the mechanism of pyroptotic cell death Lorenzo Sborgi Biozentrum, University Basel, Switzerland Search for more papers by this author Sebastian Rühl Estefania Mulvihill orcid.org/0000-0002-7074-2371 Department Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich, Joka Pipercevic Rosalie Heilig Henning Stahlberg orcid.org/0000-0002-1185-4592 Christopher J...
The transition between soluble intrinsically disordered tau protein and aggregated in neurofibrillary tangles Alzheimer's disease is unknown. Here, we propose that species can undergo liquid-liquid phase separation (LLPS) under cellular conditions phase-separated droplets serve as an intermediate toward aggregate formation. We demonstrate phosphorylated or mutant aggregation prone recombinant undergoes LLPS, does high molecular weight phospho-tau isolated from human Alzheimer brain....
Atomic force microscopy and single-molecule spectroscopy were combined to image manipulate purple membrane patches from Halobacterium salinarum. Individual bacteriorhodopsin molecules first localized then extracted the membrane; remaining vacancies imaged again. Anchoring forces between 100 200 piconewtons for different helices found. Upon extraction, found unfold. The spectra revealed individuality of unfolding pathways. Helices G F as well E D always unfolded pairwise, whereas B C...
The nucleus makes the rules Single cells continuously experience and react to mechanical challenges in three-dimensional tissues. Spatial constraints dense tissues, physical activity, injury all impose changes cell shape. How can measure shape deformations ensure correct tissue development homeostasis remains largely unknown (see Perspective by Shen Niethammer). Working independently, Venturini et al. Lomakin now show that act as an intracellular ruler cellular variations. nuclear envelope...
Abstract Tau pathology is known to spread in a hierarchical pattern Alzheimer’s disease (AD) brain during progression, likely by trans-synaptic tau transfer between neurons. However, the species involved inter-neuron propagation remains unclear. To identify responsible for propagation, we examined uptake and properties of different derived from postmortem cortical extracts interstitial fluid tau-transgenic mice, as well human AD cortices. Here show that PBS-soluble phosphorylated...
Cell shape and motility are primarily controlled by cellular mechanics. The attachment of the plasma membrane to underlying actomyosin cortex has been proposed be important for processes involving deformation. However, little is known about actual function membrane-to-cortex (MCA) in cell protrusion formation migration, particular context developing embryo. Here, we use a multidisciplinary approach study MCA zebrafish mesoderm endoderm (mesendoderm) germ layer progenitor cells, which migrate...
Integrins require an activation step prior to ligand binding and signaling. How talin kindlin contribute these events in non-hematopoietic cells is poorly understood. Here we report that fibroblasts lacking either or failed activate β1 integrins, adhere fibronectin (FN) maintain their integrins a high affinity conformation induced by Mn(2+). Despite compromised integrin adhesion, Mn(2+) enabled talin- but not kindlin-deficient initiate spreading on FN. This isotropic was the ability of...
Gasdermin-D (GSDMD), a member of the gasdermin protein family, mediates pyroptosis in human and murine cells. Cleaved by inflammatory caspases, GSDMD inserts its N-terminal domain (GSDMDNterm) into cellular membranes assembles large oligomeric complexes permeabilizing membrane. So far, mechanisms GSDMDNterm insertion, oligomerization, pore formation are poorly understood. Here, we apply high-resolution (≤ 2 nm) atomic force microscopy (AFM) to describe how membranes. We observe inserting...
The organization and biophysical properties of the cytosol implicitly govern molecular interactions within cells. However, little is known about mechanisms by which cells regulate cytosolic intracellular diffusion rates. Here, we demonstrate that environment budding yeast undertakes a startling transition upon glucose starvation in macromolecular mobility dramatically restricted, reducing movement both chromatin nucleus mRNPs cytoplasm. This confinement cannot be explained an ATP decrease or...
Abstract Eukaryotic cells can undergo different forms of programmed cell death, many which culminate in plasma membrane rupture as the defining terminal event 1–7 . Plasma was long thought to be driven by osmotic pressure, but it has recently been shown cases an active process, mediated protein ninjurin-1 8 (NINJ1). Here we resolve structure NINJ1 and mechanism ruptures membranes. Super-resolution microscopy reveals that clusters into structurally diverse assemblies membranes dying cells,...