A5057465271- Microtubule and mitosis dynamics
- Pancreatic function and diabetes
- Mitochondrial Function and Pathology
- Cellular Mechanics and Interactions
- Fungal and yeast genetics research
- Force Microscopy Techniques and Applications
- Amino Acid Enzymes and Metabolism
- Genomics and Chromatin Dynamics
- Metabolism, Diabetes, and Cancer
- Enzyme Structure and Function
- Diabetes and associated disorders
- Microbial Metabolic Engineering and Bioproduction
- Polyamine Metabolism and Applications
- Diet, Metabolism, and Disease
- Erythrocyte Function and Pathophysiology
- Chromosomal and Genetic Variations
- Protein Structure and Dynamics
- Cellular transport and secretion
- Alzheimer's disease research and treatments
- Genetic Neurodegenerative Diseases
- Microfluidic and Bio-sensing Technologies
- Cancer, Hypoxia, and Metabolism
- Cell Image Analysis Techniques
- Endoplasmic Reticulum Stress and Disease
- RNA and protein synthesis mechanisms
Kurume University
2015-2025
Max Planck Institute of Molecular Cell Biology and Genetics
2010-2019
Cell Biotech (South Korea)
2016-2017
Max Planck Society
2008-2013
Kyoto University
2002-2011
Meijo University
1978-2007
Nagoya University
1979-2004
Yamaguchi University
1995-1999
The University of Tokyo
1992
Rockefeller University
1984
Chromosome segregation and cell division are essential, highly ordered processes that depend on numerous protein complexes. Results from recent RNA interference screens indicate the identity composition of these complexes is incompletely understood. Using gene tagging bacterial artificial chromosomes, localization, tandem-affinity purification-mass spectrometry, MitoCheck consortium has analyzed about 100 human complexes, many which had not or only been characterized. This work led to...
Glucokinase (Gck) functions as a glucose sensor for insulin secretion, and in mice fed standard chow, haploinsufficiency of β cell–specific Gck (Gck+/–) causes impaired secretion to glucose, although the animals have normal cell mass. When high-fat (HF) diet, wild-type showed marked hyperplasia, whereas Gck+/– demonstrated decreased replication insufficient hyperplasia despite showing similar degree resistance. DNA chip analysis revealed receptor substrate 2 (Irs2) expression HF diet–fed...
Abstract Little is known about how mitotic cells round against epithelial confinement. Here, we engineer micropillar arrays that subject to lateral mechanical confinement similar experienced in epithelia. If generating sufficient force deform the pillars, rounding (MDCK) can create space divide. However, if cannot space, their force, which generated by actomyosin contraction and hydrostatic pressure, pushes cell out of After conducting mitosis an unperturbed manner, both daughter return...
To divide, most animal cells drastically change shape and round up against extracellular confinement. Mitotic facilitate this process by generating intracellular pressure, which the contractile actomyosin cortex directs into shape. Here, we introduce a genome-scale microcantilever- RNAi-based approach to phenotype contribution of > 1000 genes rounding single mitotic Our screen analyzes force, pressure volume localizes selected proteins. We identify 49 relevant for rounding, large portion...
Cohesin maintains sister chromatid cohesion until its Rad21/Scc1/Mcd1 is cleaved by separase during anaphase. DNA topoisomerase II (topo II) the proper topology of DNAs and essential for chromosome segregation. Here we report direct observations mitotic progression in individual HeLa cells after functional disruptions hRad21, NIPBL, a loading factor topo alpha,beta RNAi inhibitor, ICRF-193. Mitosis delayed Mad2-dependent manner disruption either or both cohesin II. In hRad21 depletion,...
Parkinson's disease is associated with mitochondrial decline in dopaminergic neurons of the substantia nigra. One genes linked onset disease, DJ-1/PARK7, belongs to a novel glyoxalase family and influences activity. It has been assumed that glyoxalases fulfill this task by detoxifying aggressive aldehyde by-products metabolism. Here we show supplying either D-lactate or glycolate, products DJ-1, rescues requirement for enzyme maintenance potential. We further glycolic acid D-lactic can...
1. Distribution of N τ -methylhistidine (3-methylhistidine; Me-His) among organs and tissues in cattle was determined. From the amount Me-His skeletal muscle protein daily urinary output Me-His, fractional catabolic synthetic rates myofibrillar proteins during growth were calculated. 2. More than 93.4% total (35.6 mg/kg body-weight) analysed occurred protein. The other relatively small. Daily excretion which fed on hay concentrate 135 mg at a growing stage 217 kg body-weight 145 312 kg....
ABSTRACT In the fission yeast Schizosaccharomyces pombe, transcriptional upregulation and cell-surface localization of hexose transporter Ght5 are required for cell proliferation in low glucose. As target rapamycin complex 2 (TORC2) signaling pathway inhibits α-arrestin Aly3-dependent endocytosis Ght5, we hypothesized that phosphorylation this endocytosis. To identify sites glucose, putatively phosphorylated serine/threonine residues Aly3 were replaced with alanine, showing C-terminal serine...
Although pericentromeric heterochromatin is essential for chromosome segregation, its role in humans remains controversial. Dissecting the function of HIV-1–encoded Vpr, we unraveled important properties during segregation. In Vpr-expressing cells, hRad21, hSgo1, and hMis12, which are crucial proper were displaced from centromeres mitotic chromosomes, resulting premature chromatid separation (PCS). Interestingly, Vpr protein 1-α (HP1-α) HP1-γ chromatin. RNA interference (RNAi) experiments...