A5080836794- Microfluidic and Bio-sensing Technologies
- Plasmonic and Surface Plasmon Research
- Microfluidic and Capillary Electrophoresis Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Nanopore and Nanochannel Transport Studies
- Photonic and Optical Devices
- Electrowetting and Microfluidic Technologies
- Advanced Fiber Optic Sensors
- Electrohydrodynamics and Fluid Dynamics
- Spectroscopy Techniques in Biomedical and Chemical Research
- Micro and Nano Robotics
- Optical Coatings and Gratings
- Mechanical Circulatory Support Devices
- 3D Printing in Biomedical Research
- Planetary Science and Exploration
- Innovative Microfluidic and Catalytic Techniques Innovation
- Magnetic and Electromagnetic Effects
- Infective Endocarditis Diagnosis and Management
- Cardiac Valve Diseases and Treatments
- Geomagnetism and Paleomagnetism Studies
- Advanced Biosensing Techniques and Applications
- Microbial Inactivation Methods
- Characterization and Applications of Magnetic Nanoparticles
Queen's University
2016-2025
Consumer VOICE
2024-2025
ETH Zurich
2012-2015
Board of the Swiss Federal Institutes of Technology
2015
Hospital Central Dr. Ignacio Morones Prieto
2013
University of Victoria
2008-2012
BC Cancer Agency
2009
Universidade Estadual de Maringá
2009
Universidad Autónoma Metropolitana
2005
University of Toronto
2001-2002
We combine nanofluidics and nanoplasmonics for surface-plasmon resonance (SPR) sensing using flow-through nanohole arrays. The role of surface plasmons on resonant transmission motivates the application arrays as surface-based biosensors. Research to date, however, has focused dead-ended holes, therefore failed harness benefits nanoconfined transport combined with SPR sensing. format enables rapid reactants active inside nanoholes, potential significantly improved time analysis biomarker...
The integration of fluidics and optics, as in flow-through nanohole arrays, has enabled increased transport analytes to sensing surfaces. Limits detection, however, are fundamentally limited by local analyte concentration. We employ the array geometry conducting nature film actively concentrate within sensor. achieve 180-fold enrichment a dye, 100-fold simultaneous protein less than 1 min. method presents opportunities for an order magnitude increase speed 2 orders improvement limit detection.
Bacterial adhesins are modular cell-surface proteins that mediate adherence to other cells, surfaces, and ligands. The Antarctic bacterium Marinomonas primoryensis uses a 1.5-MDa adhesin comprising over 130 domains position it on ice at the top of water column for better access oxygen nutrients. We have reconstructed this 0.6-μm-long using "dissect build" structural biology approach established complementary roles its five distinct regions. Domains in region I (RI) tether type secretion...
We quantify the efficacy of flow-through nanohole sensing, as compared to established flow-over format, through scaling analysis and numerical simulation. Nanohole arrays represent a growing niche within surface plasmon resonance-based sensing methods, employing nanoholes nanochannels can enhance transport analytical response. The additional benefit offered by operation is, however, complex function operating parameters application-specific binding chemistry. Compared here are sensors array...
Abstract Little is known about how mitotic cells round against epithelial confinement. Here, we engineer micropillar arrays that subject to lateral mechanical confinement similar experienced in epithelia. If generating sufficient force deform the pillars, rounding (MDCK) can create space divide. However, if cannot space, their force, which generated by actomyosin contraction and hydrostatic pressure, pushes cell out of After conducting mitosis an unperturbed manner, both daughter return...
We present an original, low-cost nanoplasmonic (bio)sensor based on crossed surface relief gratings (CSRGs) generated from orthogonally superimposed (SRGs) gold-coated azo-glass substrate. This plasmon resonance (SPR)-based sensing approach is unique, since the light transmitted through a CSRG zero except in narrow bandwidth where SPR conversion occurs, enabling quantitative monitoring of only plasmonic signal biomolecular interactions real time. validated individual SRG signature CSRGs by...
Nanohole array-based biosensors integrated with a microfluidic concentration gradient generator were used for imaging detection and quantification of ovarian cancer markers. Calibration curves based on controlled concentrations the analyte created using stepped diffusive mixing scheme. Quantification samples unknown was achieved by image-intensity comparison calibration curves. The first to detect immobilization marker antibodies, subsequently applied r-PAX8 (with limit about 5 nM dynamic...
We present a method for the surface-enhanced Raman scattering (SERS)-based detection of toxic contaminants in minimally processed liquid food products, through use dendritic silver nanostructure, produced electrokinetic assembly nanoparticles from solution. The nanostructure is on surface microelectrode chip, connected to an AC field with imposed DC bias. apply this chip thiram, fruit pesticide, apple juice, limit 115 ppb, no sample preprocessing. also melamine, contaminant/food additive,...
The fabrication and on-chip integration of surface-enhanced Raman scattering (SERS) optrodes are presented. In the optrode configuration, both laser excitation back-scattered signal transmitted through same optical fiber. SERS-active component was fabricated self-assembly silver nanoparticles on tip fibers. application SERS to detect dyes in aqueous solution indicated a limit quantification below 1 nM, using nile blue A as molecular probe. Using optrode-integrated microfluidic chip, it...
Surface-enhanced Raman scattering (SERS) can provide ultrasensitive detection of chemical and biological analytes down to the level a single molecule. The need for costly, nanostructured, noble-metal substrates, however, poses major obstacle in widespread application method. Here we present first time novel type metallic nanostructured substrates that, not only exhibit remarkable SERS activity, but are also produced facile, cost-effective nanofabrication-free manner. formed through an...
Magnetotactic bacteria (MTB) play an important role in Earth's biogeochemical cycles by transporting minerals aquatic ecosystems, and have shown promise for controlled transport of microscale objects flow conditions. However, how MTB traverse complex environments is not clear. Here, using microfluidics high-speed imaging, it revealed that magnetotaxis enables directed motion Magnetospirillum magneticum over long distances velocities ranging from 2 to 1260 µm s-1 , corresponding shear rates...
In this paper, we demonstrate a compact integrated nanohole array-based surface plasmon resonance sensing device. The unit includes LED light source, driving circuitry, CCD detector, microfluidic network and computer interface, all assembled from readily available commercial components. A dual-wavelength scheme was implemented to increase spectral diversity isolate intensity variations be expected in the field. prototype shows bulk sensitivity of 266 pixel units/RIU limit detection 6 × 10−4...
The unique plasmonic energy exchange occurring within metallic crossed surface relief gratings (CSRGs) enables Surface Plasmon Resonance Imaging for the label-free detection of whole uropathogenic bacteria.
Metallic nanoparticles (MNPs) and metallic nanostructures are both commonly used, independently, as SERS substrates due to their enhanced plasmonic activity. In this work, we introduce investigate a hybrid nanostructure with strong activity that benefits from the collective response of combination MNPs flow-through nanohole arrays (NHAs). The electric field distribution electromagnetic enhancement factor structures composed silver NPs on gold NHAs investigated via finite-difference...
The stochastic nature of biological systems makes the study individual cells a necessity in biology. Yet, handling and disruption single analysis relatively low concentrations their protein components still challenges available techniques. Transmission electron microscopy (TEM) allows for proteins at single-molecule level. Here, we present system single-cell lysis under light observation, followed by rapid uptake cell lysate. Eukaryotic were grown on conductively coated glass slides observed...