A5084172910- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Electronic Packaging and Soldering Technologies
- Product Development and Customization
- Force Microscopy Techniques and Applications
- Design Education and Practice
- Mechanical and Optical Resonators
- Manufacturing Process and Optimization
- Advanced Electron Microscopy Techniques and Applications
- Advanced MEMS and NEMS Technologies
- Flexible and Reconfigurable Manufacturing Systems
- Lipid Membrane Structure and Behavior
- Electron and X-Ray Spectroscopy Techniques
- Enzyme Structure and Function
- Receptor Mechanisms and Signaling
- Mass Spectrometry Techniques and Applications
- Additive Manufacturing and 3D Printing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Pharmacological Effects and Assays
- Protein Structure and Dynamics
- Electrohydrodynamics and Fluid Dynamics
- Ion Transport and Channel Regulation
- Analytical Chemistry and Sensors
- Photonic and Optical Devices
- RNA and protein synthesis mechanisms
University of Basel
2014-2024
Centre for Human Drug Research
2023
Leiden University
2023
Broadcom (United States)
2022-2023
Intel (United States)
2022-2023
Auburn University
2022-2023
MicroVision (United States)
2022-2023
Gorgias Press (United States)
2022-2023
Marko-Kolor (Poland)
2023
Marvell (United States)
2022-2023
This paper focuses on the hardware aspects of battery management systems (BMS) for electric vehicle and stationary applications. The purpose is giving an overview existing concepts in state-of-the-art enabling reader to estimate what has be considered when designing a BMS given application. After short analysis general requirements, several possible topologies packs their consequences BMS’ complexity are examined. Four that were taken from commercially available vehicles shown as examples....
We present a sample preparation method for cryo-electron microscopy (cryo-EM) that requires only 3–20 nL of to prepare cryo-EM grid, depending on the protocol used. The is applied and spread grid by microcapillary. procedure does not involve any blotting steps, real-time monitoring allows water film thickness be assessed decreased an optimum value prior vitrification. demonstrate suitable high-resolution will enable alternative electron approaches, such as single-cell visual proteomics.
Micromechanical cantilever arrays are used to measure time-resolved adsorption of tiny masses based on protein-ligand interactions. Here, streptavidin-biotin interactions investigated in a physiological environment. A measurement method is introduced using higher flexural modes silicon order enhance the sensitivity mass detection. Modeling vibration liquid allows absolute changes. We show final $7\ifmmode\pm\else\textpm\fi{}0.7\phantom{\rule{0.3em}{0ex}}\mathrm{ng}$ biotinylated latex beads....
We evaluated the potential and limitations of resonating nanomechanical microcantilevers for detection mass adsorption. As a test system we used addition gold layers varying thickness. Our main findings are: (1) A linear increase in sensitivity with square mode number—a two orders magnitude is obtained from 1 to 7 minimum 8.6 ag Hz−1 µm−2 resolution 0.43 pg at µm thick cantilever. (2) The quality factor increases number, thus helping achieve higher sensitivity. (3) effective spring constant...
A study of nanomechanical cantilevers vibrating at various resonating modes in liquid is presented. Resonant frequency spectrum with 16 well resolved flexural obtained. The quality factor increased from 1 mode to 30 16. theoretical estimate eigenfrequency using the Elmer–Dreier model [F.-J. Elmer and M. Dreier, J. Appl. Phys. 81, 12 (1997)] Sader’s extended viscous [C. A. Van Eysden E. Sader, 101, 044908 (2007)] matched experimental data. apparent mass comoved by oscillating decreased...
The authors present the prototype of a chemical sensor using capacitive micromachined ultrasonic transducer array. Each element in array consists large number resonating membranes connected parallel. A five-channel oscillator circuit operates at resonant frequency around 6MHz this prototype. surface elements is coated by polymers such as polyallylamine hydrochloride, polyethylene glycol, and polyvinyl alcohol to detect different chemicals. By measuring shift oscillation frequencies due...
A microcantilever based method for fluid viscosity and mass density measurements with high temporal resolution microliter sample consumption is presented. Nanomechanical cantilever vibration driven by photothermal excitation detected an optical beam deflection system using two laser beams of different wavelengths. The theoretical framework relating response to the surrounding was extended consider higher flexural modes vibrating at Reynolds numbers. performance developed sensor theory...
High-resolution structural information is essential to understand protein function. Protein-structure determination needs a considerable amount of protein, which can be challenging produce, often involving harsh and lengthy procedures. In contrast, the several thousand few million particles required for structure by cryogenic electron microscopy (cryo-EM) provided miniaturized systems. Here, we present microfluidic method rapid isolation target its direct preparation cryo-EM. Less than 1 μL...
Polymers are often used to modify surface properties control interfacial processes. Their sensitivity solvent conditions and ability undergo conformational transitions makes polymers attractive in tailoring with specific functionalities leading applications diverse areas ranging from tribology colloidal stability medicine. A key example is polyethylene glycol (PEG), which widely as a protein-resistant coating given its low toxicity biocompatibility. We report here microcantilever-based...
Most methods used to determine the viscosity and mass density of liquids have two major drawbacks: relatively high sample consumption (∼milliliters) long measurement time (∼minutes). Resonant nanomechanical cantilevers promise overcome these limitations. Although has already been significantly reduced, resolution was rarely addressed date. We present a method decrease user interaction required for such measurements. It features (i) droplet-generating automatic sampler using fluorinated oil...
The stochastic nature of biological systems makes the study individual cells a necessity in biology. Yet, handling and disruption single analysis relatively low concentrations their protein components still challenges available techniques. Transmission electron microscopy (TEM) allows for proteins at single-molecule level. Here, we present system single-cell lysis under light observation, followed by rapid uptake cell lysate. Eukaryotic were grown on conductively coated glass slides observed...
We used microfabricated cantilever array sensors for an artificial nose setup. Each is coated on its top surface with a polymer layer. Volatile gaseous analytes are detected by tracking the diffusion process of molecules into layers, resulting in swelling layers and therewith bending cantilevers. From pattern all cantilevers array, characteristic 'fingerprint' analyte obtained, which evaluated using principal component analysis. In flow dry nitrogen gas, reverted to initial state before...
Amphiphilic di- and triblock copolymers having different hydrophilic-to-hydrophobic block length ratio were synthesized using ATRP. The self-assembly behavior of these AB ABA consisting poly(n-butyl methacrylate) (B) poly(2,2-(dimethylaminoethyl (A) was investigated a combination dynamic light scattering, negative-stain transmission electron microscopy, cryoelectron atomic force microscopy. Two populations self-organized structures in aqueous solution, micelles compound micelles, detected...
Demands to improve the sensitivity and measurement speed of dynamic scanning force microscopy cantilever sensing applications necessitate development smaller sensors. As a result, methods directly drive cantilevers, such as photothermal or magnetic excitation, are gaining in importance. Presented is report on effect excitation microcantilevers increase steady‐state temperature dynamics higher mode vibrations. First, local upon continuous irradiation with laser light at different positions...
Electron microscopy (EM) entered a new era with the emergence of direct electron detectors and nanocrystal diffraction methods. However, sample preparation techniques have not progressed still suffer from extensive blotting steps leading to massive loss sample. Here, we present simple but versatile method for almost lossless conditioning nanoliter volumes biological samples EM, keeping under close physiological condition. A microcapillary is used aspirate 3–5 nL The tip immersed into...