We report a microarray of cantilevers to detect multiple unlabeled biomolecules simultaneously at nanomolar concentrations within minutes. Ligand-receptor binding interactions such as DNA hybridization or protein recognition occurring on microfabricated silicon generate nanomechanical bending, which is detected optically in situ . Differential measurements including reference an array eight sensors can sequence-specifically targets 80-fold excess nonmatching background and discriminate 3′ 5′...

We demonstrate continuous label-free detection of two cardiac biomarker proteins (creatin kinase and myoglobin) using an array microfabricated cantilevers functionalized with covalently anchored anti-creatin anti-myoglobin antibodies. This method allows to be detected via measurement surface stress generated by antigen–antibody molecular recognition. Reference are used eliminate thermal drifts, undesired chemical reactions turbulences from injections liquids calculating differential...

We report a microcantilever-based immunosensor operated in static deflection mode with performance comparable surface plasmon resonance, using single-chain Fv (scFv) antibody fragments as receptor molecules. As model system scFv specificity to two different antigens were applied. introduced cysteine residue at the C terminus of each construct allow covalent attachment gold-coated sensor interfaces directed orientation. Application an array enabled simultaneous measurements sensing and...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFormation and in Situ Modification of Monolayers Chemisorbed on Ultraflat Template-Stripped Gold SurfacesPeter Wagner, Martin Hegner, Hans-Joachim Guentherodt, Giorgio SemenzaCite this: Langmuir 1995, 11, 10, 3867–3875Publication Date (Print):October 1, 1995Publication History Published online1 May 2002Published inissue 1 October 1995https://pubs.acs.org/doi/10.1021/la00010a043https://doi.org/10.1021/la00010a043research-articleACS...

The controlled deposition of functional layers is the key to converting nanomechanical cantilevers into chemical or biochemical sensors. Here, we introduce inkjet printing as a rapid and general method coat cantilever arrays efficiently with various sensor layers. Self-assembled monolayers alkanethiols were deposited on selected Au-coated rendered them sensitive ion concentrations pH in liquids. detection gene fragments was achieved sensors coated thiol-linked single-stranded DNA oligomers...

Miniaturized microfabricated sensors have enormous potential in gas detection, biochemical analysis, medical applications, quality and process control, product authenticity issues. Here, we highlight an ultrasensitive mechanical way of converting (bio-)chemical or physical processes into a recordable signal using cantilever arrays.

The polymerization of individual RecA–DNA filaments, containing either single-stranded or double-stranded DNA, was followed in real time, and their mechanical properties were characterized with force-measuring laser tweezers. It found that the stretch modulus a filament is dominated by its (central) DNA component, while bending rigidity controlled (eccentric) protein component. longitudinal stiffness increases 6- to 12-fold when contained helix. Both fiber change presence various nucleotide...

Micromechanical cantilever arrays are used to measure time-resolved adsorption of tiny masses based on protein-ligand interactions. Here, streptavidin-biotin interactions investigated in a physiological environment. A measurement method is introduced using higher flexural modes silicon order enhance the sensitivity mass detection. Modeling vibration liquid allows absolute changes. We show final $7\ifmmode\pm\else\textpm\fi{}0.7\phantom{\rule{0.3em}{0ex}}\mathrm{ng}$ biotinylated latex beads....

We evaluated the potential and limitations of resonating nanomechanical microcantilevers for detection mass adsorption. As a test system we used addition gold layers varying thickness. Our main findings are: (1) A linear increase in sensitivity with square mode number—a two orders magnitude is obtained from 1 to 7 minimum 8.6 ag Hz−1 µm−2 resolution 0.43 pg at µm thick cantilever. (2) The quality factor increases number, thus helping achieve higher sensitivity. (3) effective spring constant...

ABSTRACT A rapid biosensor for the detection of bacterial growth was developed using micromechanical oscillators coated in common nutritive layers. The change resonance frequency as a function increasing mass on cantilever array forms basis scheme. calculated sensitivity according to mechanical properties sensor is ∼50 pg/Hz; this corresponds an approximate ∼100 Escherichia coli cells. able detect active E. cells within 1 h. starting number initially attached was, average, ∼1,000...

A study of nanomechanical cantilevers vibrating at various resonating modes in liquid is presented. Resonant frequency spectrum with 16 well resolved flexural obtained. The quality factor increased from 1 mode to 30 16. theoretical estimate eigenfrequency using the Elmer–Dreier model [F.-J. Elmer and M. Dreier, J. Appl. Phys. 81, 12 (1997)] Sader’s extended viscous [C. A. Van Eysden E. Sader, 101, 044908 (2007)] matched experimental data. apparent mass comoved by oscillating decreased...

Significance How proteins fold natively with efficient fidelity while being synthesized remains largely unexplored. Understanding protein synthesis on a single-molecule level is of particular interest to the life sciences and relevant for various diseases. Although folding are well-studied subjects, cotranslational has been proven difficult observe. Using optical tweezers, we measured mechanics simultaneous in real time. We found that occurs at predictable locations, exerting forces nascent...

Atomic force microscopy (AFM) is a technique to image surfaces with unprecedented vertical and lateral resolution. Many related techniques have been derived from AFM, taking advantage of local interactions between tip on cantilever surface. However, cantilevers can also be used for sensing applications. These so-called nanosensors feature extreme sensitivity the detection chemical vapours or adsorption molecules. Upon surface, molecules cause bend. Thus physical, biochemical processes are...

Thiols, dialkylsulfides, and dialkyldisulfides are known to be chemisorbed with high affinity on gold. We have prepared DNAs of specific length sequence carrying thiol groups at each end. For this purpose, primers an HS‐(CH 2 ) 6 ‐arm the 5'‐end were used amplify segments plasmid DNA via polymerase chain reaction. These thiolated bind strongly large, ultraflat Au surfaces which we recently described [Hegner, M. et al. (1993) Surface Sci. 291, 39‐46], can imaged by AFM in liquids (aqueous...

Free-standing cantilevers, which directly translate specific biochemical reactions into micromechanical motion, have recently attracted much attention as label-free biosensors and micro/nano robotic devices. To exploit this mechanochemical sensing technology, it is essential to develop a fundamental understanding of the origins surface stress. Here we report detailed study molecular basis stress generation in aqueous environments focusing on pH titration model mercaptohexadecanoic acid...