We report a microcantilever-based immunosensor operated in static deflection mode with performance comparable surface plasmon resonance, using single-chain Fv (scFv) antibody fragments as receptor molecules. As model system scFv specificity to two different antigens were applied. introduced cysteine residue at the C terminus of each construct allow covalent attachment gold-coated sensor interfaces directed orientation. Application an array enabled simultaneous measurements sensing and...

Binding of integrin receptors to extracellular ligands is a complex process involving receptor-ligand interactions at the cell-substrate interface, signals activating receptors, and assembly cytoskeletal adhesion plaque proteins cytoplasmic face. To analyze contribution these elements overall cell adhesion, we have developed model system that characterizes functional binding characteristic for as force required separate integrin-ligand bond. A spinning disk device was used apply range...

The cytoplasmic domain of β3integrin contains tyrosines at positions 747 and 759 in domains that have been implicated regulation αvβ3function serve as potential substrates for Src family kinases. phosphorylation level β3 integrin was modulated using a temperature-sensitive v-Src kinase. Increased β3phosphorylation abolished αvβ3- but not α5β1-mediated adhesion to fibronectin. αvβ3-Mediated cell restored by the expression containing Y747F or Y759F mutations wild type integrin. Thus, is...

Polymers are often used to modify surface properties control interfacial processes. Their sensitivity solvent conditions and ability undergo conformational transitions makes polymers attractive in tailoring with specific functionalities leading applications diverse areas ranging from tribology colloidal stability medicine. A key example is polyethylene glycol (PEG), which widely as a protein-resistant coating given its low toxicity biocompatibility. We report here microcantilever-based...

We present an approach for sensing protein aggregation using microcantilever systems. Results from both single cantilever experiments with internal reference and multicantilever array measurements dedicated cantilevers are discussed. show that in cases on the sensor can be detected through associated changes surface stress.

Recent advancements in nanomechanical microcantilever biosensors open new possibilities for clinical applications, permitting precise analysis of molecular interactions. The technology enables tracking gene expression, conformational changes, antibody binding and antibiotic resistance. In particular, hybridization DNA or RNA extracted from biopsies whole blood patients has led to significant diagnostics critical medical conditions, e.g., cancer, bacteraemia sepsis, utilizing rapid,...

Cell surface integrins can adopt distinct conformations in response to ligand binding and intracellular signals. Several including alpha(v)beta(3) bind multiple ligands. The of fibronectin vitronectin was used as a model determine whether the same or forms receptor were utilized strong two different A spinning-disc device measure relative strength alpha(v)beta(3)-ligand bonds. initial reaction for both ligands occurred absence metabolic energy resulted adhesion but weak vitronectin....

The mannose transporter of the bacterial phosphotransferase system mediates carbohydrate transport across cytoplasmic membrane concomitant with phosphorylation. It also functions as a receptor for chemotaxis [Adler, J. & Epstein, W. (1974) Proc. Natl Acad. Sci. USA 71 , 2895–2899] and is required infection cell by bacteriophage λ where it most likely pore penetration phage DNA [Elliott, Arber, (1978) Mol. Gen. Genet. 161 1–8]. consists two transmembrane subunits (27‐kDa IIC Man 31‐kDa...

Integrin receptors serve as mechanical links between the cell and its structural environment. Using alpha(v)beta3 integrin expressed in K562 cells a model system, process by which connection vitronectin develops was analyzed measuring resistance of these bonds to separation. Three distinct stages activation, defined increases alpha(v)beta3-vitronectin binding strength, were mutational, biochemical, biomechanical analyses. Activation low strength stage 1 occurs through interaction with ligand...

A new microcantilever array design is investigated comprising eight flexible microcantilevers introducing two solid bars, enabling to subtract contributions from differences in refractive index an optical laser read out system. Changes the do not contribute undesirably bending signals at picomolar micromolar DNA or protein concentrations. However, measurements of samples with high salt concentrations serum are affected, requiring corrections for artifacts. Moreover, obtain a deeper...

A cantilever-based protein biosensor has been developed providing a customizable multilayer platform for the detection of antibodies. It consists biotin-terminated PEG layer pre-functionalized on gold-coated cantilever surface, onto which NeutrAvidin is adsorbed through biotin/NeutrAvidin specific binding. used as bridge between biotin-coated surface and biotinylated biomolecules, such bovine serum albumin (biotinylated BSA), forming sensor direct antibody capture. The successfully applied...

According to the American skin cancer foundation, there are more new cases of than combined incidence cancers breast, prostate, lung, and colon each year, malignant melanoma represents its deadliest form. About 50% all characterized by a particular mutation BRAF(V600E) in BRAF (Rapid Acceleration Fibrosarcoma gene B) gene. Recently developed highly specific drugs able fight mutated tumors but require diagnostic tools for fast reliable detection warrant treatment efficiency. We completed...

Sepsis is a life-threatening condition mostly caused by bacterial infection resulting in inflammatory reaction and organ dysfunction if not treated effectively. Rapid identification of the causing pathogen already early stage bacteremia therefore vital. Current technologies still rely on time-consuming procedures including culturing up to 72 h. Our approach based ultra-rapid highly sensitive nanomechanical sensor arrays. In measurements we observe two clearly distinguishable distributions...