A5023564164- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Autophagy in Disease and Therapy
- Glycosylation and Glycoproteins Research
- Cancer-related Molecular Pathways
- Peptidase Inhibition and Analysis
- Microtubule and mitosis dynamics
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Cellular transport and secretion
- Cancer-related gene regulation
- Enzyme Structure and Function
- 14-3-3 protein interactions
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Mitochondrial Function and Pathology
- Genetics and Neurodevelopmental Disorders
- interferon and immune responses
- Advanced Proteomics Techniques and Applications
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Biotin and Related Studies
- Proteins in Food Systems
- Erythrocyte Function and Pathophysiology
Max Planck Institute of Biochemistry
2015-2025
Research Network (United States)
2022-2025
University of Wisconsin–Madison
2025
St. Jude Children's Research Hospital
2014-2024
Technical University of Munich
2023-2024
Howard Hughes Medical Institute
2010-2019
University of Tennessee Health Science Center
2005-2019
University of California, San Francisco
2014-2017
Max Planck Society
2015-2017
University of Tennessee at Knoxville
2015
Despite best current therapy, up to 20% of pediatric patients with acute lymphoblastic leukemia (ALL) have a relapse. Recent genomewide analyses identified high frequency DNA copy-number abnormalities in ALL, but the prognostic implications these not been defined.We studied cohort 221 children high-risk B-cell-progenitor ALL use single-nucleotide-polymorphism microarrays, transcriptional profiling, and resequencing samples obtained at diagnosis. Children known very-high-risk subtypes (i.e.,...
The papain-like protease PLpro is an essential coronavirus enzyme that required for processing viral polyproteins to generate a functional replicase complex and enable spread1,2. also implicated in cleaving proteinaceous post-translational modifications on host proteins as evasion mechanism against antiviral immune responses3–5. Here we perform biochemical, structural characterization of the severe acute respiratory syndrome 2 (SARS-CoV-2) (SCoV2-PLpro) outline differences with SARS-CoV...
Pediatric acute lymphoblastic leukemia (ALL) is a heterogeneous disease consisting of distinct clinical and biological subtypes that are characterized by specific chromosomal abnormalities or gene mutations. Mutation genes encoding tyrosine kinases uncommon in ALL, with the exception Philadelphia chromosome-positive where t(9,22)(q34;q11) translocation encodes constitutively active BCR-ABL1 kinase. We recently identified poor prognostic subgroup pediatric BCR-ABL1-negative ALL patients...
Postsynaptic differentiation of dendrites is an essential step in synapse formation. We report here a requirement for the transcription factor myocyte enhancer 2A (MEF2A) morphogenesis postsynaptic granule neuron dendritic claws cerebellar cortex. A transcriptional repressor form MEF2A that sumoylated at lysine-403 promoted claw differentiation. Activity-dependent calcium signaling induced calcineurin-mediated dephosphorylation serine-408 and, thereby, switch from sumoylation to acetylation...
Significance At the molecular level, most processes in living systems are mediated by multisubunit protein complexes. Recombinant forms of these complexes essential for analyzing their structure and function. Multigene expression constructs greatly improve recombinant complex preparations, but generation such can be a rate-limiting step. To overcome this limitation, we have adapted Gibson assembly reactions rapid, efficient, fast numerous parallel used resulting biGBac method different...
Significance PINK1 protein kinase and PARKIN UB ligase are mutated in inherited forms of Parkinson’s disease several cancers. Thus, it is great significance to understand normal functions that could be disrupted disease. A role for mediating autophagy damaged mitochondria (mitophagy) through polyubiquitylation numerous mitochondrial outer membrane proteins a reaction involves phosphorylation both ubiquitin (UB) by PINK1. The mechanism remains unclear, however, due challenges defining...
Acetylation of an amino-terminal methionine is important for mediating specific protein-protein interactions.
Autophagy is required for the homeostasis of cellular material and proposed to be involved in many aspects health. Defects autophagy pathway have been observed neurodegenerative disorders; however, no genetically-inherited pathogenic mutations any core autophagy-related (ATG) genes reported human patients date. We identified a homozygous missense mutation, changing conserved amino acid, ATG5 two siblings with congenital ataxia, mental retardation, developmental delay. The subjects' cells...