A5049751649- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Crystallography and molecular interactions
- CO2 Reduction Techniques and Catalysts
- Asymmetric Hydrogenation and Catalysis
- Carbon dioxide utilization in catalysis
- Supramolecular Chemistry and Complexes
- Metal-Organic Frameworks: Synthesis and Applications
- Enzyme Structure and Function
- Supramolecular Self-Assembly in Materials
- Organometallic Complex Synthesis and Catalysis
- Biochemical and Molecular Research
- Molecular Sensors and Ion Detection
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Porphyrin and Phthalocyanine Chemistry
- Photosynthetic Processes and Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Chemical Synthesis and Analysis
- Nuclear Physics and Applications
- Genetics and Neurodevelopmental Disorders
- Radiopharmaceutical Chemistry and Applications
- Protein Degradation and Inhibitors
University of Utah
2015-2025
St. Jude Children's Research Hospital
2014-2020
Salt Lake Regional Medical Center
2020
Howard Hughes Medical Institute
2014-2019
Significance At the molecular level, most processes in living systems are mediated by multisubunit protein complexes. Recombinant forms of these complexes essential for analyzing their structure and function. Multigene expression constructs greatly improve recombinant complex preparations, but generation such can be a rate-limiting step. To overcome this limitation, we have adapted Gibson assembly reactions rapid, efficient, fast numerous parallel used resulting biGBac method different...
Abstract Sample purity is central to in vitro studies of protein function and regulation, the efficiency success structural using techniques such as x-ray crystallography cryo-electron microscopy (cryo-EM). Here, we show that mass photometry (MP) can accurately characterize heterogeneity a sample minimal material with high resolution within matter minutes. To benchmark our approach, use negative stain electron (nsEM), popular method for EM screening. We include typical workflows developed...
Significance The ability of eukaryotic cells to pass their genomes properly from one cell generation the next depends on 1.2-MDa ubiquitin ligase complex APC/C (anaphase-promoting complex/cyclosome) and correct timing its activation by substrate adaptor CDC20 (cell division cycle 20). Although it has been known for two decades that mitotic phosphorylation is required CDC20, mechanistic basis this process remained unknown, in part because existence numerous phospho-sites made systematic...
Significance The anaphase-promoting complex/cyclosome (APC) is a multisubunit RING E3 ubiquitin (Ub) ligase that regulates mitosis, meiosis, and numerous facets of neurobiology by targeting key regulatory proteins for Ub-mediated degradation. Despite great importance, it remains unclear how APC, or most the other 600 E3s in humans, targets Ub to lysines disordered substrates. Here, we report structural molecular basis substrate ubiquitination APC its partner E2, UBCH10. UBCH10 recruited...
Hierarchical self-assembly of discrete organoplatinum(II) metallacycles has attracted considerable attention. However, the exact assembly mechanism involving non-covalent interactions limited formation and application due to dynamics platinum metallacycles. Herein, we report hierarchical a pyrene-based double-metallacycle that takes advantage heteroligation-coordination-driven triflate anions' hydrogen bonding, which is extended into 3-D supramolecular framework by hydrogen-bonding anions....
The recent progress in platinum(II) coordination-driven supramolecular polymers has had a substantial effect on the design of functional soft materials. However, prospect realizing polymerization induced by metallacycle-based host–guest interactions received little attention until recently. Here we report realization driven both solid state and solution. On basis disclosed mechanism, present new strategy for preparation polymers.
Owing to the energetic cost associated with CO2 release in carbon capture (CC), combination of and recycling (CCR) is an emerging area research. In this approach, "captured CO2," typically generated by addition amines, serves as a substrate for subsequent reduction. Herein, we report that reduction presence morpholine (generating mixtures corresponding carbamate carbamic acid) well-established Mn electrocatalyst changes product selectivity from CO H2 formate. The change attributed situ...
The hydrogenation of CO2 in the presence amines to formate, formamides, and methanol (MeOH) is a promising approach streamlining carbon capture recycling. To achieve this, understanding how catalyst design impacts selectivity performance critical. Herein we describe thorough thermochemical analysis (de)hydrogenation catalyst, (PNP)Ru–Cl (PNP = 2,6-bis(di-tert-butylphosphinomethyl)pyridine; Ru Ru(CO)(H)) correlate our findings performance. Although this known hydrogenate formate with mild...
Herein, we describe the catalytic hydrogenation of CO2 to formate with (PNP)Mn–H (PNP = 2,6-bis(di-tert-butylphosphinomethyl)pyridine; Mn Mn(CO)2). Contrary established mechanism for hydrogenation, mechanistic studies indicate that does not insert into Mn–H bond give species, (PNP)Mn–OCHO. The lack reactivity is confirmed by thermochemical show sufficiently hydridic reduce CO2. Deprotonation hydride [(*PNP)Mn–H]– (* indicates deprotonated ligand) enhances hydricity ∼17 kcal·mol–1 and hence...
A comprehensive study of the reactions chelating phosphines with Ni(cod)2 to form (phosphine)Ni(cod), (phosphine)2Ni, or mixtures thereof is presented. series (phosphine)Ni(cod) complexes were isolated and characterized. The structural differences between (phosphine)2Ni examined using X-ray crystallography 1H 31P NMR spectroscopy. In addition, effects ring size, rigidity, bulk phosphine backbone on formation either investigated. These studies show that Ni–P bond lengths in both size formed...
Abstract Catenated cages represent chemistry’s challenging synthetic targets because a three-dimensional assembly is necessary for their formation. Herein, cyclic bis[2]catenane constructed through the coordination-driven self-assembly of interlocked bis-metallacage, by 90° Pt(II) heteroligation endo-functionalized double-bridged tweezer bearing pyridyl moieties and tetra-carboxylated linker. NMR spectrometry, X-ray crystallography mass spectrometry confirm formation with “∞”-shaped topology...
Considerable progress in platinum metallacycle-based supramolecular polymerization has promoted the fabrication and application of materials. However, despite recent advances, polymers constructed through host–guest complexation remain rare because dynamics metallacycles. Here, we achieve linear via by following design rule suppressing The establishment system realization this type are expected to open opportunities for functional
Methods to access fluorinated molecules are of significant interest the medicinal and agrochemical industries. We report a series high-valent PdIV complexes stabilized by two distinct ligand cores, which mediate electrophilic fluorination reactions with excellent yields good regioselectivity. Using high-throughput experimentation kinetic analysis, roles each were uncovered. Synthetic modulation catalyst alongside density functional theory transition state modeling provided evidence into...
Polycyclic aromatic hydrocarbons (PAHs) continue to attract increasing interest with respect their applications as luminescent materials. The ordered structure of the metal-organic complex facilitates selective integration PAHs that can be tuned function cooperatively. Here, a unique highly twisted anthracene-based organoplatinum metallacycle was prepared via coordination-driven self-assembly. Single-crystal X-ray diffraction analysis revealed through cooperation strong π···π stacking...
The investigation of the stereoelectronic influence N-aryl substituted NHC ligands on Ni(0) is reported. structural analysis a family [(NHC)Ni(styrene)2] complexes are correlated to known literature parameters (TEP and %VBur). involved NMR spectroscopic techniques X-ray crystallography isolated complexes. synthesis characterization this user-friendly easy accessible precatalysts can be realized in two-step starting from deprotonation imidazolium salt followed by direct coordination onto...
Ligand p K a and metal hydricity scale with one another in (de)hydrogenation catalysts that undergo metal–ligand cooperativity, irrespective of or ligand identity. Anionic hydrides are significantly more hydridic than their neutral counterparts.
In this work, we present the formation of two open-ended hexagonal-prism tubular macrocycles by [6 + 12] self-assembly symmetric ∼120° organic ligand donor with ∼90° Pt(II) acceptor in a 1:2 ratio. The assembled structures were characterized multinuclear NMR (1H NMR, 31P{1H} and 1H-1H COSY NMR), electrospray ionization mass spectrometry (ESI-TOF-MS), traveling wave ion mobility-mass (TWIM-MS), transmission electron microscopy. Molecular modeling was further conducted to get insight into...
Bipolar redox-active molecules (BRMs) have been suggested as a means to address crossover related issues in all-organic redox flow batteries (RFBs).