A5072311064- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Advanced biosensing and bioanalysis techniques
- Animal Virus Infections Studies
- Corporate Governance and Law
- Protein Degradation and Inhibitors
- Acute Myeloid Leukemia Research
- Cellular transport and secretion
- RNA Research and Splicing
- HIV Research and Treatment
- interferon and immune responses
- Biosensors and Analytical Detection
- Bacillus and Francisella bacterial research
- Neuroscience and Neuropharmacology Research
- Diverse Legal and Medical Studies
- Digitalization, Law, and Regulation
- Histone Deacetylase Inhibitors Research
- CAR-T cell therapy research
- Genomics and Chromatin Dynamics
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Alzheimer's disease research and treatments
- Genetic and Kidney Cyst Diseases
- Chromosomal and Genetic Variations
University Hospital Frankfurt
2020-2024
Goethe University Frankfurt
2002-2024
Universitätsklinikum Erlangen
2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2024
Charité - Universitätsmedizin Berlin
2024
Karlsruhe University of Education
2023
Deutsches Institut für Normung
2023
Helmholtz Centre for Environmental Research
2023
University of Tübingen
2023
Bayerisches Landesamt für Umwelt
2023
The papain-like protease PLpro is an essential coronavirus enzyme that required for processing viral polyproteins to generate a functional replicase complex and enable spread1,2. also implicated in cleaving proteinaceous post-translational modifications on host proteins as evasion mechanism against antiviral immune responses3–5. Here we perform biochemical, structural characterization of the severe acute respiratory syndrome 2 (SARS-CoV-2) (SCoV2-PLpro) outline differences with SARS-CoV...
Abstract Due to numerous mutations in the spike protein, SARS-CoV-2 variant of concern Omicron (B.1.1.529) raises serious concerns since it may significantly limit antibody-mediated neutralization and increase risk reinfections. While a rapid number cases is being reported worldwide, until now there has been uncertainty about efficacy vaccinations monoclonal antibodies. Our vitro findings using authentic variants indicate that contrast currently circulating Delta variant, vaccine-elicited...
<h2>Summary</h2><h3>Background</h3> In recent months, Omicron variants of SARS-CoV-2 have become dominant in many regions the world, and case numbers with subvariants BA.1 BA.2 continue to increase. Due numerous mutations spike protein, efficacy currently available vaccines, which are based on Wuhan-Hu 1 isolate SARS-CoV-2, is reduced, leading breakthrough infections. Efficacy monoclonal antibody therapy also likely impaired. <h3>Methods</h3> our <i>in vitro</i> study using A549-AT cells...
The capacity of convalescent and vaccine-elicited sera monoclonal antibodies (mAb) to neutralize SARS-CoV-2 variants is currently high relevance assess the protection against infections. We performed a cell culture-based neutralization assay focusing on authentic B.1.617.1 (Kappa), B.1.617.2 (Delta), B.1.427/B.1.429 (Epsilon), all harboring spike substitution L452R. found that L452R had reduced susceptibility mAbs. Compared B.1, Kappa Delta showed by factor 8.00 5.33, respectively, which...
Abstract Whether monoclonal antibodies are able to neutralize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern has been investigated using pseudoviruses. In this study we show that bamlanivimab, casirivimab, and imdevimab efficiently authentic SARS-CoV-2, including variant B.1.1.7 (alpha), but B.1.351 (beta) P.2 (zeta) were resistant against bamlanivimab partially casirivimab. variantshas We SARS-CoV-2 carrying E484K less susceptible convalescent vaccine-elicited sera.
Abstract The IgG1 monoclonal antibody (mAb) bamlanivimab (LY-CoV555) prevents viral attachment and entry into human cells by blocking to the ACE2 receptor. However, whether is equally effective against SARS-CoV-2 emerging variants of concern (VOC) not fully known. Hence, aim this study was determine VOC. ability neutralize five including B.1.1.7 (mutations include N501Y del69/70), B.1.351 E484K N501Y) P.2 in absence a mutation) analyzed infectious cell culture using CaCo2 cells....
Currently, the viable but non-culturable (VBNC) state is an underappreciated niche for pathogenic bacteria which provides a continuous source recurrent infections and transmission. We propose VBNC to be global persistence mechanism used by various A. baumannii strains cope with many stresses it confronted in clinical environment host. This requires novel strategy detect cells of this pathogen that not only based on plating assays.
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of disease COVID-19, which has become a global concern due to its rapid spread. common methods monitor and quantitate SARS-CoV-2 infectivity in cell culture are so far time-consuming labor-intensive. Using Sleeping Beauty transposase system, we generated robust versatile cellular infection model that allows experiments compatible for high-throughput live imaging. based on lung derived A549 cells, show...
Synthesis of acetylcholine depends on the plasma membrane uptake choline by a high affinity transporter (CHT1). Choline is regulated nerve impulses and trafficking an intracellular pool CHT1 to may be important for this regulation. We have generated hemagglutinin (HA) epitope tagged investigate organelles involved with protein. Expression CHT1-HA in HEK 293 cells establishes Na+-dependent, hemicholinium-3 sensitive high-affinity transport activity. Confocal microscopy reveals that found...
Wastewater-based SARS-CoV-2 epidemiology (WBE) has been established as an important tool to support individual testing strategies. The Omicron sub-variants BA.4/BA.5 have spread globally, displacing the preceding variants. Due severe transmissibility and immune escape potential of BA.4/BA.5, early monitoring was required assess implement countermeasures in time. In this study, we monitored prevalence at six municipal wastewater treatment plants (WWTPs) Federal State North Rhine-Westphalia...
Wastewater-based epidemiology (WBE) has demonstrated its importance to support SARS-CoV-2 complementing individual testing strategies. Due their immune-evasive potential and the resulting significance for public health, close monitoring of variants concern (VoC) is required evaluate regulation early local countermeasures. In this study, we demonstrate a rapid workflow wastewater-based detection newly emerging VoCs Omicron in end 2021 at municipal wastewater treatment plant (WWTP)...
The interplay between host factors and viral components impacts replication efficiency profoundly. Members of the cellular heterogeneous nuclear ribonucleoprotein family (hnRNPs) have been extensively studied as HIV-1 dependency factors, but whether they play a role in innate immunity is currently unknown. This study aimed to identify hnRNPA0 type I interferon (IFN)-repressed factor HIV-1-infected cells. Knockdown hnRNPA0, situation that mirrors conditions under IFN stimulation, increased...
Abstract The capacity of convalescent and vaccine-elicited sera monoclonal antibodies (mAb) to neutralize SARS-CoV-2 variants is currently high relevance assess the protection against infections. We performed a cell culture-based neutralization assay focusing on authentic B.1.617.1 (Kappa), B.1.617.2 (Delta), B.1.427/B.1.429 (Epsilon), all harboring spike substitution L452R. found that L452R had reduced susceptibility mAbs. Compared B.1, Kappa Delta showed by factor 8.00 5.33, respectively,...
Efficient HIV-1 replication depends on balanced levels of host cell components including cellular splicing factors as the family serine/arginine-rich (SRSF, 1-10). Type I interferons (IFN-I) play a crucial role in innate immunity against by inducing expression IFN-stimulated genes (ISGs) potent restriction factors. The less well known IFN-repressed (IRepGs) might additionally affect viral downregulating dependency that are essential for life cycle; however, so far, knowledge about IRepGs...
High-throughput sequencing describes multiple alterations in individual tumors, but their functional relevance is often unclear. Clinic-close, individualized molecular model systems are required for validation and to identify therapeutic targets of high significance each patient. Here, we establish a Cre-ERT2-loxP (causes recombination, estrogen receptor mutant T2, locus X-over P1) based inducible RNAi- (ribonucleic acid interference) mediated gene silencing system patient-derived xenograft...
Abstract Leukemia patients bearing the t(4;11)(q21;q23) translocations can be divided into two subgroups: those expressing both reciprocal fusion genes, and that have only MLL-AF4 gene. Moreover, a recent study has demonstrated genes better outcome than are protein alone. All this may point to clonal process where gene AF4-MLL could lost during disease progression, as loss select for more aggressive type of leukemia. Therefore, we were interested in unraveling decisive role at an early...
Abstract Wastewater-based SARS-CoV-2 epidemiology (WBE) has been established as an important tool to support individual testing strategies. Omicron sub-variants BA.4/5 have spread globally displacing the predeceasing variants. Due severe transmissibility and immune escape potential of BA.4/5, early monitoring was required asses implement countermeasures in time. In this study, we monitored prevalence at six municipal wastewater treatment plants (WWTPs) Federal State North-Rhine-Westphalia...
Wastewater-based SARS-CoV-2 epidemiology (WBE) has proven as an excellent tool to monitor pandemic dynamics supporting individual testing strategies. WBE can also be used early warning system for monitoring the emergence of novel pathogens or viral variants. However, a timely transmission results, sophisticated sample logistics and analytics performed in decentralized laboratories close sampling sites are required. Since multiple commonly use custom in-house workflows purification...
The emergence of SARS-CoV-2 Omicron subvariants prompted countries to call for accelerated booster vaccinations limit disease and transmission. Here, we characterized correlates protection over time after the second or BA.1 infection comparing variants concern (VOCs). Sera from subjects before two seven weeks were examined level Spike receptor-binding-domain (RBD)-specific antibodies. Furthermore, neutralizing antibodies (nABs) in vitro neutralization assays Alpha, Beta, Delta, BA.2 against...
Hypoxia contributes to numerous pathophysiological conditions including inflammation-associated diseases. We characterized the impact of hypoxia on immunometabolic cross-talk between cholesterol and interferon (IFN) responses. Specifically, reduced biosynthesis flux provoked a compensatory activation sterol regulatory element-binding protein 2 (SREBP2) in monocytes. Concomitantly, broad range interferon-stimulated genes (ISGs) increased under absence an inflammatory stimulus. While changes...
NESP55 (neuroendocrine secretory protein of Mr 55,000) is a paternally imprinted proteoglycan, expressed specifically in endocrine cells and the nervous system. We investigated subcellular localization secretion AtT-20 cells. accumulated medium linearly over 24 h exceeding its intracellular content 3.7-fold by that time. Incubation at 16°C, to block export, inhibited basal 79%. Stimulation with 8-Br-cAMP increased only 45%. The vesicles sedimented density 1.2–1.4 M, which slightly lighter...