A5114374340- Cytomegalovirus and herpesvirus research
- Immune Cell Function and Interaction
- Herpesvirus Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- Renal Transplantation Outcomes and Treatments
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Urinary Tract Infections Management
- Tuberculosis Research and Epidemiology
- Pediatric Urology and Nephrology Studies
- Mycobacterium research and diagnosis
- COVID-19 Clinical Research Studies
- Organ Transplantation Techniques and Outcomes
- Parvovirus B19 Infection Studies
- Urinary Bladder and Prostate Research
- Organ Donation and Transplantation
- Polyomavirus and related diseases
- Dialysis and Renal Disease Management
- Influenza Virus Research Studies
- Viral-associated cancers and disorders
- Hepatitis B Virus Studies
- HIV Research and Treatment
- Inflammasome and immune disorders
- Pneumocystis jirovecii pneumonia detection and treatment
- Cell Adhesion Molecules Research
Saarland University
2014-2023
Klinikum rechts der Isar
2023
Technical University of Munich
2023
Humboldt State University
2021
Universitätsklinikum des Saarlandes
2005-2020
PHV Dialysezentrum
2016
Giessen School of Theology
2007
Justus-Liebig-Universität Gießen
2007
Heidelberg University
2004-2007
Zero to Three
2007
Heterologous priming with the ChAdOx1 nCoV-19 vector vaccine followed by boosting a messenger RNA (BNT162b2 or mRNA-1273) is currently recommended in Germany, although data on immunogenicity and reactogenicity are not available. In this observational study we show that, healthy adult individuals (n = 96), heterologous regimen induced spike-specific IgG, neutralizing antibodies CD4 T cells, levels of which were significantly higher than after homologous boost 55) comparable magnitude to mRNA...
Background. Immunosuppressive treatment in transplant patients frequently causes infectious complications with cytomegalovirus (CMV). The extent of CMV replication can be followed by a number diagnostic methods. There is, however, no simple tool to assess the quality cellular antiviral immune response an individual patient. This would particular importance for therapy decisions, as detectable virus load do not necessarily develop CMV-related disease. Using rapid whole blood assay,...
BACKGROUND. Patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) differ in the severity of disease. We hypothesized that characteristics SARS-CoV-2–specific immunity correlate disease severity.
Abstract Comparative analyses of the immunogenicity and reactogenicity homologous heterologous SARS-CoV-2 vaccine-regimens will inform optimized vaccine strategies. Here we analyze humoral cellular immune response following vaccination strategies in a convenience cohort 331 healthy individuals. All regimens induce immunity to antigen. Immunity after with ChAdOx1-nCoV-19 followed by either BNT162b2 ( n = 66) or mRNA-1273 101) is equivalent more pronounced than mRNA-regimens 43 BNT162b2, 59...
Background. Patients on chronic intermittent haemodialysis (HD) show an impaired cellular and humoral immune response that clinically appears with frequent infectious complications low vaccination responses. This defect strongly correlates reduced in vitro proliferative responses of T cells. The is localized antigen presenting cells, which a decreased co‐stimulatory activity. Furthermore, the increased production pro‐inflammatory cytokines. In to primary activation, CD4 positive helper (Th)...
Abstract Background Cytomegalovirus (CMV) seronegative recipients (R-) of kidney transplants (KT) from seropositive donors (D+) are at higher risk for CMV replication and ganciclovir(GCV)-resistance than R(+). We hypothesized that low CMV-specific T-cell responses associated with increased in R(+)-patients D(+) or D(-) donors. Methods prospectively evaluated 73 consecutive KT-patients [48 R(+), 25 D(+)R(-)] undergoing routine testing as part a preemptive strategy. compared interferon-γ...
T-cell based IFN-γ release assays do not permit distinction of active tuberculosis (TB) from successfully treated disease or latent M. infection. We postulated that and IL-2 cytokine profiles antigen-specific T cells measured by flow-cytometry ex vivo might correlate with TB activity in vivo. Tuberculin (PPD), ESAT-6 CFP-10 were used as stimuli to determine CD4 24 patients 28 using flow-cytometry. Moreover, 25 individuals immunity consistent infection BCG-vaccination, respectively,...
Individual doses of dual-dose vaccine-regimens are sequentially administered into the deltoid muscle, but little attention has so far been paid to immunological effects choosing ipsilateral or contralateral side for second dose.In an observational study, 303 previously naive individuals were recruited, who received dose COVID-19 vaccine BNT162b2 on either (n = 147) 156). Spike-specific IgG, IgG-avidity, and neutralizing antibodies quantified using ELISA a surrogate assay 2 weeks after 2. A...
ABSTRACT Replication of cytomegalovirus (CMV) is largely controlled by the cellular arm immune response. In this study CMV-specific CD4 T-cell response was characterized in a cohort apparently healthy individuals. 11% all individuals, extremely high frequencies, between 10 and 40%, were found. High-level frequencies T cells persisted over several months not result an acute infection. Specific oligoclonal phenotypically functionally as mature effector cells, with both cytokine-secreting...
ABSTRACT Epstein-Barr virus (EBV)-induced posttransplant lymphoproliferative disease (PTLD) continues to be a serious complication following transplantation. The aim of the present study was evaluate EBV load as parameter for prediction and monitoring PTLD. analyzed by quantitative competitive PCR with 417 whole-blood samples 59 patients after allogeneic stem cell transplantation (SCT). positive all 9 PTLD 17 without viral loads manifest differed from those (median loads, 1.4 × 10 6 versus 4...
ABSTRACT. Cellular immune responses are of high importance in initiating and maintaining immunity against virus infections. Whereas the cellular response during persistent cytomegalovirus (CMV) infection is well assessable, individual contribution CD4 CD8 T cell primary has not been described. A novel whole-blood assay, which relies on flow-cytometric detection antigen-induced cytokine expression, was used to characterize CMV-specific CMV seronegative recipients a renal allograft from...
The formation of supramolecular activation clusters within the immunological synapse, crucial for sustained signaling and T lymphocyte activation, requires costimulation-dependent reorganization actin cytoskeleton. Here we have identified actin-remodeling protein cofilin as a key player in this process. Cell-permeable peptides that block costimulation-induced cofilin/F-actin interactions untransformed human lymphocytes impair receptor capping synapse at interface between cells...
Rearrangements in the actin cytoskeleton play a pivotal role for costimulation-induced formation of immunological synapse and T cell activation. Yet, little is known about actin-binding proteins that link costimulation to rearrangements cytoskeleton. Here we demonstrate phosphorylation bundling protein L-plastin response through TCR/CD3 plus CD2 or CD28, respectively, important activation human peripheral blood lymphocytes (PBT). Mass spectrometry site-directed mutagenesis revealed Ser5...
Cytomegalovirus (CMV) infection is the most frequent viral complication in patients after allogeneic stem cell transplantation. As CMV replication tightly controlled by cellular arm of specific immunity, kinetics CMV-specific T cells association with individual reactivation episodes were prospectively analyzed 40 transplant recipients a routine clinical setting and evaluated as determinant impaired control. Antigen-specific CD4 CD8 quantified directly from whole blood using intracellular...