A5070027273- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Renal Transplantation Outcomes and Treatments
- Immune Response and Inflammation
- Pancreatic and Hepatic Oncology Research
- T-cell and B-cell Immunology
- Inflammatory Bowel Disease
- Cytomegalovirus and herpesvirus research
- Cancer Research and Treatments
- Immune cells in cancer
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Signaling Pathways in Disease
- Pancreatitis Pathology and Treatment
- Cell Adhesion Molecules Research
- Microscopic Colitis
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Hedgehog Signaling Pathway Studies
- Cancer Cells and Metastasis
- Immunodeficiency and Autoimmune Disorders
- TGF-β signaling in diseases
- Cytokine Signaling Pathways and Interactions
- Adenosine and Purinergic Signaling
Heidelberg University
2016-2025
University Hospital Heidelberg
2016-2025
German Center for Infection Research
2017-2025
BARMER GEK
2016-2019
Hudson Institute
2019
John Wiley & Sons (United States)
2019
National Center for Tumor Diseases
2011-2016
Hochschule Düsseldorf University of Applied Sciences
2015
Département Santé Animale
2014
Wageningen University & Research
2014
Abstract The Toll-like receptor (TLR)9 is critical for the recognition of immunostimulatory CpG motifs but may cooperate with other TLRs. We analyzed TLR1–10 mRNA expression by using quantitative real-time PCR in highly purified subsets human PBMC and determined sensitivity these to oligodeoxynucleotides (ODN). TLR1 TLR6 were expressed all cell types examined. TLR10 was B cells weakly plasmacytoid dendritic (PDC). High TLR2 characteristic monocytes. PDC marked levels TLR7 TLR9 directly...
Human plasmacytoid dendritic cells (DC) (PDC, CD123+) and myeloid DC (MDC, CD11c+) may be able to discriminate between distinct classes of microbial molecules based on a different pattern Toll-like receptor (TLR) expression. TLR1–TLR9 were examined in purified PDC MDC. TLR9, which is critically involved the recognition CpG motifs mice, was present PDCbut not TLR4, required for response LPS, selectively expressed Consistent with TLR expression, susceptible stimulation by oligodeoxynucleotide...
Abstract Detailed information of human B cell activation via TLR may lead to a better understanding involvement in autoimmunity and malignancy. In this study we identified fundamental difference the regulation TLR7- TLR9-mediated stimulation: whereas induction polyclonal naive proliferation by TLR7 ligands resiquimod (R848) loxoribine required presence plasmacytoid dendritic cells (PDCs), TLR9 ligand CpG was independent PDCs. We found that PDC-derived type I IFN enhanced sensitivity...
Pancreatic ductal adenocarcinoma (PDAC) is characterized by extensive collagen-rich stroma. T cells that infiltrate pancreatic cancers frequently become trapped in the stroma and do not contact tumor cells. Here, we aimed to analyze how chemokines extracellular matrix (ECM) collagen interact mediating T-cell infiltration PDAC.T-cell distribution ECM structure within tumors were analyzed. Chemokine concentrations human PDAC compared with levels of immune cell infiltration. We assessed...
Steroid-resistant nephrotic syndrome (SRNS) causes 15% of chronic kidney disease cases. A mutation in 1 over 40 monogenic genes can be detected approximately 30% individuals with SRNS whose symptoms manifest before 25 years age. However, many patients, the genetic etiology remains unknown. Here, we have performed whole exome sequencing to identify recessive SRNS. In 7 families and facultative ichthyosis, adrenal insufficiency, immunodeficiency, neurological defects, identified 9 different...
Through the agnostic screening of patients with uncharacterised disease phenotypes for an upregulation type I interferon (IFN) signalling, we identified a cohort individuals heterozygous mutations in PTPN1, encoding protein-tyrosine phosphatase 1B (PTP1B). We aimed to describe clinical phenotype and molecular cellular pathology this new disease. In case series, collected neuroradiological data through collaboration paediatric neurology genetics colleagues across Europe (Czechia, France,...
Abstract Two different CpG oligonucleotides (ODN) were used to study the regulation of type I IFN in human plasmacytoid dendritic cells (PDC): ODN 2216, a CpG-A ODN, known induce high amounts IFN-α PDC, and 2006, CpG-B which is potent at stimulating B cells. showed higher prolonged kinetics production compared with that ODN. In contrast, was more active than IL-8 increasing costimulatory Ag-presenting molecules, suggesting trigger distinct regulatory pathways PDC. Indeed, but not activated...
We recently showed that mucus from patients with ulcerative colitis, a chronic inflammatory disorder of the colon, is characterized by low level phosphatidylcholine (PC) while clinical studies reveal therapeutic addition PC using slow release preparations beneficial. The positive role in this disease still elusive. Here we tested hypothesis exogenous application has anti-inflammatory properties three model systems. First, human Caco-2 cells were treated tumor necrosis factor-α (TNF-α) to...
BackgroundIt has been suggested that Crohn's disease (CD) is associated with an exaggerated T-helper 1 cytokine response manifested by increased production of interleukin (IL)-12. IL-12 a heterodimeric protein comprising 2 disulfide-linked subunits designated p35 and p40. Recently, IL-12-related cytokines, IL-23 IL-27, were described. Biologically active heterodimer whose p40 subunit identical to IL-12p40 whereas its p19 distantly related IL-12p35. IL-27 consists EBI3, IL-12p40-related...
Abstract Heme oxygenase-1 (HO-1) is believed to represent a key enzyme for the protection of cells against “stress.” Its overexpression in different types human cancers supports notion that HO-1 provides growth advantage and contributes cellular resistance chemotherapy radiotherapy. Given poor survival rates patients with pancreatic cancer due its aggressive behavior exceptional all known forms anticancer treatment, we have investigated expression prognosis. Expression was analyzed samples...
Background and Purpose— Acute ischemic stroke in humans is associated with profound alterations the immune system. Hallmarks of this stroke-induced immunodepression syndrome are: lymphocytopenia, impairment T helper cell monocyte function. We studied which stroke-specific factors predict these immunologic subsequent infections. Methods— Leukocyte/lymphocyte subsets were assessed serially by white blood count fluorescence-activated sorter analysis patients (n=50) at baseline, day 1, 4 after...
Abstract Atherosclerosis is a chronic inflammatory disease. Lesion progression primarily mediated by cells of the monocyte/macrophage lineage. IL-17A proinflammatory cytokine, which modulates immune cell trafficking and involved inflammation in (auto)immune infectious diseases. But role still remains controversial. In current study, we investigated effects on advanced murine human atherosclerosis, common disease phenotype clinical care. The 26-wk-old apolipoprotein E–deficient mice were fed...
Pancreatic ductal adenocarcinoma (PDAC) is frequently associated with fibrosis and a prominent inflammatory infiltrate in the desmoplastic stroma. Moreover, PDAC, an epithelial-to-mesenchymal transition (EMT) observed. To explore possible connection between infiltrating cells, particularly polymorphonuclear neutrophils (PMN) tumor cell transition, biopsies of patients PDAC (n = 115) were analysed regard to PMN infiltration nuclear expression β-catenin ZEB1, well-established indicators EMT....