Abstract Wastewater-based epidemiology (WBE) is a great approach that enables us to comprehensively monitor the community determine scale and dynamics of infections in city, particularly metropolitan cities with high population density. Therefore, we monitored time course SARS-CoV-2 RNA concentration raw sewage Frankfurt area, European financial center. To sewage, continuously collected 24 h composite samples twice week from two wastewater treatment plant (WWTP) influents (Niederrad...

This study investigated the potential of Membrane-Aerated Biofilm Reactors (MABRs) for mainstream nitrogen removal via partial nitration/anaerobic ammonium oxidation (anammox). Four laboratory-scale MABRs were operated with real municipal wastewater characterized by low concentrations (varying between 31 and 120 mg-NH

Treated wastewater (TWW) irrigation is a useful counter-measure against the depletion of freshwater (FW) resources. However, TWW contains several contaminants emerging concern, such as antibiotic resistant bacteria (ARB) and genes (ARGs). Thus, might promote spread antimicrobial resistance in soil environments. In present work, we hypothesized that ARG load intensity define effect on dynamics soil. This hypothesis was tested using multiphase approach: a) comparing from full-scale,...

Wastewater-based severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) surveillance of Frankfurt Airport by genome sequencing was used to detect SARS-CoV-2 variants entering the region. In November 2021, we found all characteristic mutations Omicron in wastewater originating from before first confirmed clinical report an arriving passenger on 26 2021.

Abstract PCR-based methods have caused a surge for integration of eco-physiological approaches into research on partial nitritation anammox (PNA). However, lack rigorous standards molecular analyses resulted in widespread data misinterpretation and consequently consensus. Data consistency accuracy strongly depend the primer selection interpretation. An in-silico evaluation 16S rRNA gene eubacterial primers used PNA studies from last ten years unraveled difficulty comparing ecological...

In the present study, we investigated impact of treated wastewater (TWW) irrigation on prevalence antibiotic resistance genes (ARGs) in subsoil pore-water, a so-far under-appreciated matrix. We hypothesized that TWW increases ARG pore-water. This hypothesis was tested using multiphase approach, which consisted sampling percolated pore-water from lysimeter-wells real-scale TWW-irrigated field, operated for commercial farming practices, and controlled, laboratory microcosms irrigated with...

We report a sequencing analysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater samples collected the Frankfurt, Germany, metropolitan area. The majority detected mutations have been identified only clinical genomes outside indicating that SARS-CoV-2 can provide insights into emerging variants city.

Wastewater-based SARS-CoV-2 epidemiology (WBE) has been established as an important tool to support individual testing strategies. The Omicron sub-variants BA.4/BA.5 have spread globally, displacing the preceding variants. Due severe transmissibility and immune escape potential of BA.4/BA.5, early monitoring was required assess implement countermeasures in time. In this study, we monitored prevalence at six municipal wastewater treatment plants (WWTPs) Federal State North Rhine-Westphalia...

Wastewater-based epidemiology (WBE) has demonstrated its importance to support SARS-CoV-2 complementing individual testing strategies. Due their immune-evasive potential and the resulting significance for public health, close monitoring of variants concern (VoC) is required evaluate regulation early local countermeasures. In this study, we demonstrate a rapid workflow wastewater-based detection newly emerging VoCs Omicron in end 2021 at municipal wastewater treatment plant (WWTP)...

Abstract Wastewater based surveillance employing qPCR has already shown its utility for monitoring SARS-CoV-2 at community level, and consequently the European Commission recommended implementation of an EU Sewage Sentinel System. However, using sequencing determination genomic variants in wastewater is not fully established yet. Therefore, we focused on analysis RNA samples collected across 20 countries including 54 municipalities. Our results provide unprecedented insight into abundance...

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemia has been one of the most difficult challenges humankind recently faced. Wastewater-based epidemiology emerged as a tool for surveillance and mitigation potential viral outbreaks, circumventing biases introduced by clinical patient testing. Due to situation urgency, protocols followed isolating RNA from sewage were not adapted such sample matrices. In parallel their implementation fast collection data sustain decisions,...

Abstract Background Sequencing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA from wastewater samples has emerged as a valuable tool for detecting the presence and relative abundances SARS-CoV-2 variants in community. By analyzing viral genetic material present wastewater, researchers public health authorities can gain early insights into spread virus lineages emerging mutations. Constructing reference datasets known their mutation profiles become state-of-the-art...

Abstract The substantial presence of denitrifiers has already been reported in partial nitritation anammox (PNA) systems using the 16S ribosomal RNA (rRNA) gene, but little is known about phylogenetic diversity based on denitrification pathway functional genes. Therefore, we performed a metagenomic analysis to determine distribution genes and associated phylogeny PNA whether niche separation between conventional activated sludge (AS) exists. results revealed distinct abundance pattern their...

Abstract Background Sequencing of SARS-CoV-2 RNA from wastewater samples has emerged as a valuable tool for detecting the presence and relative abundances variants in community. By analyzing viral genetic material present wastewater, public health officials can gain early insights into spread virus inform timely intervention measures. The construction reference datasets known lineages their mutation profies become state-of-the-art assigning sequencing data. However, selection sequences or...

Abstract There are increasing concerns about wastewater treatment plants (WWTPs) acting as hotspots for antibiotic resistance genes (ARG). However, their role largely depends upon the methods and antibiotics in wastewater. To better understand these influences, we compared occurrence fate of ARG between a pond system developing country (Namibia) an advanced WWTP (activated sludge system) developed (Germany). A targeted metagenomic approach was used to investigate wide-spectrum profiles ARGs...

Tracking SARS-CoV-2 variants in wastewater is primarily performed by detecting characteristic mutations of the variants. Unlike Delta variant, emergence Omicron variant and its sublineages as concern has posed a challenge using for surveillance. In this study, we monitored temporal spatial variation including all detected compared whether limiting analyses to like impact outcomes. We collected 24-hour composite samples from 15 treatment plants (WWTP) Hesse sequenced 164 with targeted...

Abstract Wastewater-based epidemiology (WBE) is a great approach that enables us to comprehensively monitor the community determine scale and dynamics of infections in city, particularly metropolitan cities with high population density. Therefore, we monitored time course SARS-CoV-2 RNA concentration raw sewage Frankfurt area, European financial center. To sewage, continuously collected samples from two wastewater treatment plant (WWTP) influents (Niederrad Sindlingen) serving area performed...

Abstract Waste stabilization ponds (WSP) exist worldwide to treat wastewater, especially in warm climates. They are characterized by simple operation and maintenance over 50 years many WSP were built urban communities Namibia. This study evaluated nine of these systems terms their influent effluent water quality compared them with the requirements for reuse agriculture. In current state none adhered Namibian or new European standards, due tCOD concentrations above 100 mg/L caused high algal...

Summary The choice of primer and TaqMan probes to quantify ammonia‐oxidizing bacteria (AOB) in environmental samples is crucial importance. re‐evaluation pairs based on current genomic sequences used for quantification the amoA gene revealed (1) significant misrepresentations AOB population samples, (2) a lack perfect match Nitrosomonas europaea eutropha . We designed two new cluster 7‐specific N. (nerF/nerR/nerTaq) (netF/netR/netTaq). Specificity biases newly sets were compared with most...

Using metagenome sequencing, a nearly complete genome sequence was retrieved for the uncultured Methyloceanibacter sp. strain A49, recovered from an activated sludge system used landfill leachate treatment at closed site. The total size and encoded sequences are 3,407,434 bp 3,280 genes, respectively.

Upcycling wastes into valuable products by mixed microbial communities has recently received considerable attention. Sustainable production of high-value substances from one-carbon (C1) compounds, e.g., methanol supplemented as an external electron donor in bioreactors for wastewater treatment, is a promising application upcycling. This study undertook gene-centric approach to screen potentials culture biomass, removing organic carbon and nitrogen landfill leachate. To this end, the...

Abstract Waste stabilization pond (WSP) systems exist in many countries affected by climate change causing increasing demand for irrigation water. There is little long-term experience with WSP Africa, and thus, this study provides a comprehensive investigation of Namibia. One its treatment trains was upgraded mechanical anaerobic pre-treatment, post-treatment compared to second train without upgrades. The pre-treatment showed positive results on COD, TSS partially pathogen removal. E. coli...