A5088860868- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- RNA Interference and Gene Delivery
- Neuroscience and Neural Engineering
- Autism Spectrum Disorder Research
- Genetics and Neurodevelopmental Disorders
- Silk-based biomaterials and applications
- CAR-T cell therapy research
- Polymer Surface Interaction Studies
- Fungal and yeast genetics research
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Research and Treatments
- Nanofabrication and Lithography Techniques
- Caveolin-1 and cellular processes
- Plant Reproductive Biology
- Biochemical and Structural Characterization
- Energy Harvesting in Wireless Networks
- stochastic dynamics and bifurcation
- Phagocytosis and Immune Regulation
- Receptor Mechanisms and Signaling
- Cancer, Stress, Anesthesia, and Immune Response
- Lipid Membrane Structure and Behavior
- Cell Adhesion Molecules Research
University of Zurich
2021-2023
California Institute of Technology
2021
ETH Zurich
2013-2020
Roche (United States)
2020
Board of the Swiss Federal Institutes of Technology
2016-2019
Stanford University
2012
Columbia University
2010
Teneurins are evolutionarily conserved transmembrane receptors that function as axon guidance and target selection molecules in the developing nervous system. How teneurins recognize each other, whether they establish neuronal adhesion, which teneurin specific interactions guide neurons remains to be determined. To reveal insight into these pertinent questions we combine atomic force microscopy-based single-cell spectroscopy with genetic engineering quantify between animal cells. Using a...
Microorganisms have evolved specific cell surface molecules that enable discrimination between cells from the same and a different kind. Here, we investigate role of Flo11-type adhesins social yeasts in kin discrimination. We measure adhesion forces mediated by Flo11A-type domains using single-cell force spectroscopy, quantify Flo11A-based aggregation populations determine Flo11A-dependent segregation competing yeast strains biofilms. find Flo11A diverse species confer remarkably strong...
The goal of cancer-drug delivery is to achieve high levels therapeutics within tumors with minimal systemic exposure that could cause toxicity. Producing biologics directly in situ where they diffuse and act locally an attractive alternative direct administration recombinant therapeutics, as secretion by the tumor itself provides local concentrations a paracrine fashion continuously over extended duration (paracrine delivery). We have engineered SHielded, REtargeted ADenovirus (SHREAD) gene...
Single-cell force spectroscopy (SCFS) is becoming a widely used method to quantify the adhesion of living cell substrate, another or tissue. The high sensitivity SCFS permits determining contributions individual molecules (CAMs) an entire cell. However, prepare adherent cells for SCFS, they must first be detached from tissue-culture flasks plates. EDTA and trypsin are often applied this purpose. Because cellular properties can affected by treatment, need recover before being further...
Abstract An important question is how growing tissues establish a blood vessel network. Here we study vascular network formation in pancreatic islets, endocrine derived from epithelium. We find that depletion of integrin-linked kinase (ILK) the epithelial cells mice results glucose intolerance due to loss intra-islet vasculature. In turn, vessels accumulate at islet periphery. Neither alterations endothelial cell proliferation, apoptosis, morphology, Vegfa expression and VEGF-A secretion nor...
Fragile X syndrome (FXS) is caused by a repression of the FMR1 gene that codes mental retardation protein (FMRP), an RNA binding involved in processes are crucial for proper brain development. To better understand consequences absence FMRP, we analyzed expression profiles and activities cortical neural progenitor cells (NPCs) neurons obtained from FXS patients' induced pluripotent stem (IPSCs) IPSC-derived knock-out engineered using CRISPR-CAS9 technology. Multielectrode array recordings...
Longer and stronger: Engineered multivalent polypeptides were used to increase tune the adhesion strength of cells surfaces. Monodisperse containing programmable valencies a cell-adhesion sequence synthesized (with up 80 repeats RGD sequence; see schematic illustration). The provided strong resistance cellular delamination under shear. Detailed facts importance specialist readers are published as ”Supporting Information”. Such documents peer-reviewed, but not copy-edited or typeset. They...
Abstract Tissue clearing combined with deep imaging has emerged as a powerful technology to expand classical histological techniques. Current techniques have been optimized for sparsely pigmented organs such the mammalian brain. In contrast, melanin-rich tissue, of great interest in investigation melanomas, remains challenging. To address this challenge, we developed CRISPR-based gene editing approach that is easily incorporated into existing tissue-clearing workflows PACT method. We term...
Abstract The lack of fragile X mental retardation protein (FMRP) protein, due to a repression the FMR1 gene, causes Fragile syndrome (FXS), one most prevalent forms syndromic autisms. gene codes for an RNA binding involved in regulation expression through processing, control local translation, and protein-protein interactions; processes that are crucial proper brain development. Taking advantage induced pluripotent stem cells (iPSCs) CRISPR-Cas9 genome editing technologies, we generated...
Cell adhesion to surfaces has applications in biomaterials science, surface chemistry, and micropatterning technologies. Control of this process is currently achieved using a limited set molecules, including fibronectin its derived peptide RGD. Here, we use recombinant strategies create multivalent, monodisperse polypeptides containing up 80 repeats By varying the number repeats, are able tune cells surfaces. Cells on these modified further showed strong resistance cell delamination under...