A5103177794- Retinal Diseases and Treatments
- Retinal and Macular Surgery
- Glaucoma and retinal disorders
- Retinal Imaging and Analysis
- Intraocular Surgery and Lenses
- Amyotrophic Lateral Sclerosis Research
- Retinal and Optic Conditions
- Retinal Development and Disorders
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Corneal surgery and disorders
- Ocular Diseases and Behçet’s Syndrome
- Parkinson's Disease Mechanisms and Treatments
- CRISPR and Genetic Engineering
- Ocular Oncology and Treatments
- Pluripotent Stem Cells Research
- Apelin-related biomedical research
- Lysosomal Storage Disorders Research
- Vascular Malformations Diagnosis and Treatment
- Cardiovascular Disease and Adiposity
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- RNA Research and Splicing
- Wastewater Treatment and Nitrogen Removal
- Ocular Surface and Contact Lens
- Neurological Disease Mechanisms and Treatments
McGill University
2009-2025
Montreal Neurological Institute and Hospital
2021-2025
Nanchang University
2016-2025
Qingdao University
2023-2024
Second Affiliated Hospital of Nanchang University
2013-2023
Heilongjiang Institute of Technology
2022
North Sichuan Medical University
2022
Université de Montréal
2016
McGill University Health Centre
2007-2016
Montreal General Hospital
2008-2014
Misfolded glycoproteins synthesized in the endoplasmic reticulum (ER) are degraded by cytoplasmic proteasomes, a mechanism known as ERAD (ER-associated degradation). In present study, we demonstrate that of misfolded genetic variant-null Hong Kong α1-antitrypsin is enhanced overexpression ER processing α1,2-mannosidase (ER ManI) HEK 293 cells, indicating importance ManI glycoprotein quality control. We showed previously EDEM, an enzymatically inactive mannosidase homolog, interacts with and...
Antibodies are a key resource in biomedical research yet there no community-accepted standards to rigorously characterize their quality. Here we develop procedure validate pre-existing antibodies. Human cell lines with high expression of target, determined through proteomics database, modified CRISPR/Cas9 knockout (KO) the corresponding gene. Commercial antibodies against target purchased and tested by immunoblot comparing parental KO. Validated used definitively identify most highly...
The methodology for site-directed editing of single nucleotides in the vertebrate genome is considerable interest research biology and medicine. clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 type II (Cas9) system has emerged as a simple inexpensive tool genomic loci variety animal models. In zebrafish, error-prone non-homologous end joining (NHEJ) been used method to disrupt gene function. We sought develop easily create site-specific SNPs...
To dissect the role of vascular endothelial growth factor receptor-2 (VEGFR2) in Müller cells and its effect on neuroprotection diabetic retinopathy (DR), we disrupted VEGFR2 mouse glia determined cell survival, neuronal integrity, trophic production retinas. Diabetes was induced with streptozotocin. Retinal function measured electroretinography. densities were assessed morphometric immunohistochemical analyses. Loss caused a gradual reduction glial density, which reached to significant...
A hexanucleotide (GGGGCC)n repeat expansion in C9orf72 causes amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), eliciting toxic effects through generation of RNA foci, dipeptide proteins, and/or loss protein. Defects nucleocytoplasmic transport (NCT) have been implicated as a pathogenic mechanism underlying toxicity. Here, we show that disrupts the Ran-GTPase gradient NCT vitro vivo. disruption vivo is enhanced by presence compositionally different types cytoplasmic...
To investigate the effect of glucose transporter-1 (GLUT1) inhibition on diabetic retinopathy, we divided forty-eight mice into scrambled siRNA, and GLUT1 siRNA (intravitreally injected) groups. Twenty-one weeks after diabetes induction, calculated retinal concentrations, used electroretinography (ERG) histochemical methods to assess photoreceptor degeneration, conducted immunoblotting, leukostasis vascular leakage assays estimate microangiopathy. The exhibited higher concentrations than but...
Abstract Oligodendrocytes (OLs) are key players in the central nervous system, critical for formation and maintenance of myelin sheaths insulating axons, ensuring efficient neuronal communication. In last decade, use human induced pluripotent stem cells (iPSCs) has become essential recapitulating understanding differentiation role OLs vitro. Current methods include overexpression transcription factors rapid OL generation, neglecting complexity lineage development. Alternatively, growth...
<title>Abstract</title> Background Variants in the <italic>CTSB</italic> gene encoding lysosomal hydrolase cathepsin B (catB) are associated with increased risk of Parkinson’s disease (PD). However, neither specific variants driving these associations nor functional pathways that link catB to PD pathogenesis have been characterized. CatB activity contributes protein degradation and regulates signaling processes involved autophagy lysosome biogenesis. Previous <italic>in vitro</italic>...
Urotensin II (UII) is a potent vasoactive peptide that binds to the urotensin receptor-coupled receptor-14 (known as UT) and exerts wide range of actions in humans experimental animals. We tested hypothesis UII gene deletion or UT blockade ameliorate atherosclerosis.We observed significant reduction weight gain, visceral fat, blood pressure, circulating plasma lipids, proatherogenic cytokines improvement glucose tolerance knockout mice compared with wild type (P<0.05). Deletion after an...
MicroRNA-21 (miR-21) was reported to act as an oncogene during the development of many human tumors. However, little revealed about function miR-21 in retinoblastoma (RB). In this study, we examined expression RB tissues and explored relationship between phosphatase tensin homolog (PTEN)/phosphatidylinositol-3-OH kinase (PI3K)/AKT signal. Quantitative real-time PCR (qRT-PCR) results showed that level higher than retinal normal tissues. Weri-Rb-1 cells, inhibitor suppressed cell viability,...
Retinoblastoma is the most frequent intraocular malignant tumor type to occur in childhood. MicroRNA (miR)‑101‑3p has been reported function as a suppressor various types of cancer. However, biological and underlying mechanisms miR‑101‑3p retinoblastoma are largely unknown. In present study, it was identified that downregulated retinoblastoma. MTT flow cytometry assays demonstrated ectopic overexpression significantly inhibited cell viability cycle progression WERI‑Rb‑1 Y79 cells. vivo mouse...
Intercellular transmission of α-synuclein contributes to Parkinson's disease pathology. Yet, the mechanisms spread are not fully understood. Here, we used live-cell microscopy examine impact associated lipid alterations on release. We discovered that increased glucosylceramides induce ectosome shedding from primary neurons, and dopaminergic neurons derived patient iPSCs harboring mutations in GBA1 (N370S, L444P W378G) LRRK2 (G2019S R1441H) compared their isogenic control. show elevated...
Corneal diseases present a significant challenge to global health. Given the uneven distribution of ophthalmic resources, development system facilitate remote diagnosis corneal is particularly crucial. In this study, we developed an artificial intelligence named Gancor, based on large-scale clinical dataset comprising 9669 anterior segment (AS) images and corresponding fluorescein staining (CFS) from Affiliated Eye Hospital Nanchang University, as well 967 pairs AS-CFS captured via...
This study aims to develop and validate a deep learning-based automated diagnostic system that utilizes fluorescein angiography (FFA) images for the rapid accurate diagnosis of diabetic retinopathy (DR) its complications. We collected 19,031 FFA from 2753 patients between June 2017 March 2024 construct evaluate our analytical framework. The were preprocessed annotated training validating learning model. employed two-stage system: first stage used EfficientNetB0 five-class classification task...
PURPOSE: To investigate lenticule decentration following small incision extraction (SMILE) via the pupil center or tear film mark centration method and compare induction of corneal higher order aberrations (HOAs) between two methods. METHODS: This study analyzed values obtained from tangential topography difference maps 100 eyes (100 patients) undergoing SMILE with (n = 50) method. Total HOAs component were measured preoperatively 6 months postoperatively. Relationships magnitudes induced...