Jos Käfer

ORCID: 0000-0002-0561-8008
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About
Contact & Profiles
Research Areas
  • Plant and animal studies
  • Plant Reproductive Biology
  • Chromosomal and Genetic Variations
  • Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
  • Cellular Mechanics and Interactions
  • Ecology and Vegetation Dynamics Studies
  • Horticultural and Viticultural Research
  • Genetic diversity and population structure
  • Evolution and Paleontology Studies
  • Neurobiology and Insect Physiology Research
  • Fermentation and Sensory Analysis
  • 3D Printing in Biomedical Research
  • Plant Virus Research Studies
  • Coral and Marine Ecosystems Studies
  • Plant Parasitism and Resistance
  • Plant Molecular Biology Research
  • Protist diversity and phylogeny
  • Plant Diversity and Evolution
  • Pickering emulsions and particle stabilization
  • CRISPR and Genetic Engineering
  • Genetic Mapping and Diversity in Plants and Animals
  • Marine Biology and Environmental Chemistry
  • Genetics and Plant Breeding
  • Pleistocene-Era Hominins and Archaeology
  • Micro and Nano Robotics

Centre National de la Recherche Scientifique
2015-2024

Institut de Recherche pour le Développement
2022-2024

Université de Montpellier
2023-2024

Institut des Sciences de l'Evolution de Montpellier
2023-2024

Université Claude Bernard Lyon 1
2014-2023

Laboratoire de Biométrie et Biologie Evolutive
2014-2023

Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2023

Diversité, adaptation et développement des plantes
2022-2023

Agropolis International
2022

Forum Réfugiés - Cosi
2020

Cell aggregates are a tool for in vitro studies of morphogenesis, cancer invasion, and tissue engineering. They respond to mechanical forces as complex rather than simple liquid. To change an aggregate's shape, cells have overcome energy barriers. If cell shape fluctuations active enough, the aggregate spontaneously relaxes stresses ("fluctuation-induced flow"). not, changing requires sufficiently large applied stress ("stress-induced capture this distinction, we develop model based on their...

10.1073/pnas.0902085106 article EN Proceedings of the National Academy of Sciences 2009-09-26

Because of the resemblance many epithelial tissues to densely packed soap bubbles, it has been suggested that surface minimization, which drives bubble packing, could be governing cell packing as well. We test this by modeling shape cells in a Drosophila retina ommatidium. use observed configurations and shapes wild-type flies, well flies with different numbers per ommatidia, mutants where E- or N-cadherin is either deleted misexpressed. find minimization insufficient model experimentally...

10.1073/pnas.0704235104 article EN Proceedings of the National Academy of Sciences 2007-11-15

† Background and Aims About 6 % of an estimated total 240 000 species angiosperms are dioecious.The main precursors this sexual system thought to be monoecy gynodioecy.A previous angiosperm-wide study revealed that many dioecious have evolved through the pathway; some case studies a large body theoretical research also provide evidence in support gynodioecy pathway.If plants pathway, gynodioecious should co-occur same genera.However, date, no large-scale analysis has been conducted determine...

10.1093/aob/mcu134 article EN Annals of Botany 2014-08-04

We propose a probabilistic framework to infer autosomal and sex-linked genes from RNA-seq data of cross for any sex chromosome type (XY, ZW, UV). Sex chromosomes (especially the non-recombining repeat-dense Y, W, U, V) are notoriously difficult sequence. Strategies have been developed obtain partially assembled sequences. Most them remain apply numerous non-model organisms, either because they require reference genome, or designed evolutionarily old systems. Sequencing (parents progeny) by...

10.1093/gbe/evw172 article EN cc-by-nc Genome Biology and Evolution 2016-08-01

Cannabis sativa –derived tetrahydrocannabinol (THC) production is increasing very fast worldwide. C. a dioecious plant with XY Chromosomes, and only females (XX) are useful for THC production. Identifying the sex chromosome sequence would improve early sexing better management of this crop; however, genome projects have failed to do so. Moreover, as dioecy in Cannabaceae family ancestral, chromosomes potentially old thus interesting study, little known about chromosomes. Here, we...

10.1101/gr.251207.119 article EN cc-by-nc Genome Research 2020-02-01

A key step in domestication of the grapevine was transition from separate sexes (dioecy) wild Vitis vinifera ssp. sylvestris (V. sylvestris) to hermaphroditism cultivated sativa vinifera). It is known that V. has an XY system and a modified Y haplotype (Yh) sex locus small, but it not previously been precisely characterized.We generate high-quality de novo reference genome for sylvestris, onto which we map whole-genome re-sequencing data cross locate locus. Assembly full X, Y, Yh haplotypes...

10.1186/s13059-020-02131-y article EN cc-by Genome biology 2020-09-06

Estimating time-dependent rates of speciation and extinction from dated phylogenetic trees extant species (timetrees), determining how why they vary, is key to understanding ecological evolutionary processes shape biodiversity. Due an increasing availability trees, a growing number process-based methods relying on the birth-death model have been developed in last decade address variety questions macroevolution. However, this methodological progress has regularly criticized such that one may...

10.1093/sysbio/syab083 article EN cc-by-nc Systematic Biology 2021-10-05

Abstract In angiosperms, dioecious clades tend to have fewer species than their nondioecious sister clades. This departure from the expected equal richness in standard clade test has been interpreted as implying that diversify less and initiated a series of studies suggesting dioecy might be an 'evolutionary dead end‘. However, two us recently showed ‘equal richness‘ null hypothesis is not valid case derived char acters, such dioecy, proposed new for comparisons; preliminary results, using...

10.1111/jeb.12385 article EN Journal of Evolutionary Biology 2014-05-03

We recently described, in Cannabis sativa, the oldest sex chromosome system documented so far plants (12-28 Myr old). Based on estimated age, we predicted that it should be shared by its sister genus Humulus, which is known also to possess XY chromosomes. Here, used transcriptome sequencing of an F1 family H. lupulus identify and study chromosomes this species using probabilistic method SEX-DETector. identified 265 sex-linked genes lupulus, preferentially mapped C. sativa X chromosome. Using...

10.1111/nph.17456 article EN New Phytologist 2021-05-12

10.1658/1100-9233(2004)015[0647:caoivi]2.0.co;2 article EN Journal of Vegetation Science 2004-01-01

Abstract: Question: How should species cover be weighted when calculating average indicator values of vegetation relevés? Location: The Netherlands. Method: Various weighting methods were statistically investigated with 188 relevés from Netherlands for which accurate groundwater levels available. For each method the correlation between Ellenberg value moisture and mean spring level was calculated. A permutation test on coefficients revealed whether differences significant or not. Results:...

10.1111/j.1654-1103.2004.tb02306.x article EN Journal of Vegetation Science 2004-02-24

When spreading onto a protein microlattice living cells spontaneously acquire simple shapes determined by the lattice geometry. This suggests that, on lattice, cells' are in thermodynamic metastable states. Using model at equilibrium we able to reproduce observed shapes. We build phase diagram based two adimensional parameters characterizing essential cellular properties involved spreading: cell's compressibility and fluctuations.

10.1103/physrevlett.105.128101 article EN Physical Review Letters 2010-09-13

In the plant genus Silene, separate sexes and sex chromosomes are believed to have evolved twice. Silene species that wholly or largely hermaphroditic assumed represent ancestral state from which dioecy evolved. This assumption is important for choice of outgroup inferring genetic chromosomal changes involved in evolution dioecy, but mainly based on data a single locus (ITS). To establish order events more clearly, inform choice, we therefore carried out (i) multi-nuclear-gene phylogenetic...

10.1371/journal.pone.0021915 article EN cc-by PLoS ONE 2011-08-10

Comparing species richness in sister clades that differ a character state is one of the ways to study factors influencing diversification. While most its applications have focussed on traits increase diversification, some been used association trait with lower richness, e.g., occurrence dioecy flowering plants. We show here, using simulations and an analytical model, null expectation equal generally clade comparisons wrong case derived occurring independently from speciation: should expect...

10.1093/sysbio/syu024 article EN Systematic Biology 2014-03-26

Silene latifolia represents one of the best-studied plant sex chromosome systems. A new approach using RNA-seq data has recently identified hundreds sex-linked genes in this species. However, is expected to miss that are either not expressed or at low levels tissue(s) used for RNA-seq. Therefore other independent approaches needed discover such genes.Here we 10 well-characterized S. and their homologs vulgaris, a species without chromosomes, screen BAC libraries both We isolated sequenced 4...

10.1186/s12864-015-1698-7 article EN cc-by BMC Genomics 2015-07-24

Differential movement of individual cells within tissues is an important yet poorly understood process in biological development. Here we present a computational study cell sorting caused by combination adhesion and chemotaxis, where assume that all respond equally to the chemotactic signal. To capture our model mesoscopic properties cells, such as their size deformability, use Cellular Potts Model, multiscale, cell-based Monte Carlo model. We demonstrate rich array cell-sorting phenomena,...

10.1371/journal.pcbi.0020056 article EN cc-by PLoS Computational Biology 2006-06-05

We propose an analytical model for the statistical mechanics of shuffled two-dimensional foams with moderate bubble size polydispersity. It predicts without any adjustable parameters correlations between number sides $n$ bubbles (topology) and their areas $A$ (geometry) observed in experiments numerical simulations foams. Detailed statistics show that cellular patterns correlates better $\sqrt{A}$ (as claimed by Desch Feltham) than Lewis widely assumed literature). At level whole foam,...

10.1103/physrevlett.107.168304 article EN Physical Review Letters 2011-10-14

Reduced visibility of women in science is thought to be one the causes their underrepresentation among scientists, particular at senior positions.Visibility achieved through publications, and conference attendance presentations.Here, we investigated gender differences annual meetings Society Molecular Biology Evolution.The analysis meeting programs showed a regular increase female speakers for last 16 years.Data on abstract submission suggest that there are no gender-related preferences...

10.1093/gbe/evy056 article EN cc-by-nc Genome Biology and Evolution 2018-03-01

Summary The genetic basis and evolution of sex determination in dioecious plants is emerging as an active area research with exciting advances genome sequencing analysis technologies. As the sole species within sister lineage to all other extant flowering plants, Amborella trichopoda important model for understanding development flowers. Plants typically produce only male or female flowers, but mechanisms are unknown species. Sequence data derived from natural origin F1 mapping population...

10.1111/nph.17662 article EN New Phytologist 2021-08-03

A two-dimensional (2D) foam can be characterised by its distributions of bubble area and number sides. Both have an average a width (standard deviation). There are therefore at least two very different ways to characterise the disorder. The former is geometrical measurement, while latter purely topological. We discuss common points differences between both quantities. measure them in which sheared, so that bubbles move past each other ‘shuffled’ (a notion we discuss). quantities strongly...

10.1080/09500830802334249 article EN Philosophical Magazine Letters 2008-09-01

A bstract Estimating time-dependent rates of speciation and extinction from dated phylogenetic trees extant species (timetrees), determining how why they vary, is key to understanding ecological evolutionary processes shape biodiversity. Due an increasing availability trees, a growing number process-based methods relying on the birth-death model have been developed in last decade address variety questions macroevolution. However, this methodological progress has regularly criticised such...

10.1101/2021.01.04.424672 preprint EN cc-by-nc bioRxiv (Cold Spring Harbor Laboratory) 2021-01-04
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