A5080380454- Cancer Immunotherapy and Biomarkers
- Radiomics and Machine Learning in Medical Imaging
- Neuroblastoma Research and Treatments
- Radiopharmaceutical Chemistry and Applications
- Pancreatic and Hepatic Oncology Research
- Tuberculosis Research and Epidemiology
- AI in cancer detection
- Immune Response and Inflammation
- Genetic factors in colorectal cancer
- Single-cell and spatial transcriptomics
- Inflammatory Bowel Disease
- Helicobacter pylori-related gastroenterology studies
- Cancer Genomics and Diagnostics
- Colorectal Cancer Screening and Detection
- Immune cells in cancer
- Diagnosis and treatment of tuberculosis
- Eosinophilic Esophagitis
- Mycobacterium research and diagnosis
- CAR-T cell therapy research
- Microscopic Colitis
- Gastrointestinal disorders and treatments
- Ferroptosis and cancer prognosis
- Cancer Cells and Metastasis
- Cancer Diagnosis and Treatment
- Gastric Cancer Management and Outcomes
Mass General Brigham
2023-2025
Harvard University
2015-2025
PathAI (United States)
2022-2024
University of Rochester Medical Center
2017-2024
SignPath Pharma (United States)
2024
Massachusetts General Hospital
2024
Critical Path Institute
2022-2024
University of Rochester
2018-2023
Case Western Reserve University
2007-2018
The University of Texas Health Science Center at Houston
2018
There is increasing evidence that innate and adaptive immune responses are intimately linked. This linkage in part mediated through the recognition of conserved microbial products by Toll-like receptors (TLRs). Detection TLRs can result induction inflammatory cytokines activation professional antigen-presenting cells, thereby enhancing responses. Here, we show human beta-defensin-3 (hBD-3), an antimicrobial peptide, induce expression costimulatory molecules CD80, CD86, CD40, on monocytes...
Metastatic dissemination of ovarian tumors involves the invasion tumor cell clusters into mesothelial lining peritoneal cavity organs; however, tumor-specific factors that allow cancer cells to spread are unclear. We used an in vitro assay models initial step metastasis, clearance layer, examine ability a large panel both established and primary cells. Comparison gene protein expression profiles clearance-competent clearance-incompetent revealed mesenchymal genes enriched populations display...
Although abundant myeloid cell populations in the pancreatic ductal adenocarcinoma (PDAC) microenvironment have been postulated to suppress antitumor immunity, composition of these populations, their spatial locations, and how they relate patient outcomes are poorly understood.
Endocrine pathologists are reconsidering whether tumors characterized as noninvasive follicular variant of papillary thyroid carcinoma (NFVPTC) warrant a diagnosis carcinoma. A change in terminology would affect cytology diagnoses; thus, our aim was to study the preceding fine-needle aspiration (FNA) diagnoses this group tumors.We evaluated FNA primary cohort 72 consecutively resected NFVPTCs and cytologic molecular features an additional 39 that included both classical carcinomas...
Ulcerative colitis is a chronic inflammatory bowel disease that characterized by relapsing and remitting course. Assessment of activity critically informs treatment decisions. In addition to endoscopic remission, histologic remission emerging as target key factor in the evaluation therapeutic efficacy. However, manual pathologist semiquantitative limited granularity. Machine learning approaches are increasingly being developed aid pathologists accurate reproducible scoring histology,...
Over 80% of pancreatic ductal adenocarcinoma (PDA) patients are diagnosed with non-resectable late-stage disease that lacks effective neoadjuvant therapies. Stereotactic body radiation therapy (SBRT) has shown promise as an emerging approach for treating PDA, and here, we report its combination local interleukin-12 (IL-12) microsphere (MS) immunotherapy results in marked tumor reduction cures multiple preclinical mouse models PDA. Our findings demonstrate increase intratumoral interferon...
The success of Mycobacterium tuberculosis as a pathogen relies on its ability to regulate the host immune response. M. can manipulate adaptive T cell responses indirectly by modulating antigen-presenting (APC) function or directly interacting with cells. Little is known about role molecules in direct regulation function. Using biochemical approach, we identified lipoproteins LprG and LpqH major lysate responsible for costimulation primary human CD4(+) In absence APCs, activation memory cells...
Background: Histological changes induced by gluten in the duodenal mucosa of patients with non-coeliac sensitivity (NCGS) are poorly defined. Objectives: To evaluate structural and inflammatory features NCGS compared to controls coeliac disease (CeD) milder enteropathy (Marsh I-II). Methods: Well-oriented biopsies 262 control cases normal gastroscopy histologic findings, 261 CeD, 175 from 9 contributing countries were examined. Villus height (VH, μm), crypt depth (CrD, villus-to-crypt ratios...
Colorectal carcinoma (CRC) is the second leading cause of cancer mortality worldwide. CRC stratified into two major groups: microsatellite stable (MSS) and instability-high (MSI-H). MSS constitutes majority cases, has worse overall prognosis, thus far failed to respond immunotherapies targeting immune checkpoint receptors PD-1, PD-L1, CTLA-4. Here we examined alternate immunotherapy targets Tim-3 Lag-3, as well on cells in a cohort using immunohistochemistry flow cytometry together with...
Abstract While alterations in nucleus size, shape, and color are ubiquitous cancer, comprehensive quantification of nuclear morphology across a whole-slide histologic image remains challenge. Here, we describe the development pan-tissue, deep learning-based digital pathology pipeline for exhaustive detection, segmentation, classification utility this morphologic biomarker discovery. Manually-collected annotations were used to train an object detection segmentation model identifying nuclei,...
Massive gastric polyposis is a rare entity that often associated with juvenile syndrome (JPS). The aim of this study was to evaluate the clinicopathological features 22 patients abundant juvenile-type or hyperplastic-like polyps.The included 12 males and 10 females median age 48 years (range: 13-79 years). Fourteen (64%) carried diagnosis JPS, three had prior gastrointestinal adenocarcinomas. Patients without known JPS presented at an older (60 versus 40 years; P = 0.0068). Clinical symptoms...
Several different types of non-conventional dysplasia have been recently described in inflammatory bowel disease [IBD]. Hypermucinous, goblet cell-deficient and crypt cell dysplasias received most attention, but there is limited information regarding their clinicopathological features clinical outcomes.A total 126 cases hypermucinous [n = 55], 26] 45] from 97 IBD patients were collected seven institutions analysed.The cohort included 62 [64%] men 35 [36%] women with a mean age 49 years...
Naked mole rats (NMRs) are the longest-lived rodents yet their stem cell characteristics remain enigmatic. Here, we comprehensively mapped NMR hematopoietic landscape and identified unique features likely contributing to longevity. Adult NMRs form red blood cells in spleen marrow, which comprise a myeloid bias toward granulopoiesis together with decreased B-lymphopoiesis. Remarkably, youthful marrow single-cell transcriptomes compositions largely maintained until at least middle age. Similar...
Aims Lymphocytic oesophagitis (LE) is a histological pattern characterised by increased peripapillary lymphocytes and absent or rare granulocytes. Although its association with Crohn's disease (CrD) established in children, little known about LE adult CrD patients. Previous studies have demonstrated immunophenotypical heterogeneity of LE, suggesting clinical utility distinguishing CD8 T cell‐predominant associated gastroesophageal reflux from CD4 other aetiologies. This study aimed to...
Mycobacterium tuberculosis causes persistent infection due to its ability evade host immune responses. M. induces Toll-like receptor 2 (TLR2) signaling, which influences responses TLR2 agonists expressed by include lipoproteins (e.g., LprG), the glycolipid phosphatidylinositol mannoside 6 (PIM6), and lipoglycan lipomannan (LM). Another lipoglycan, mannose-capped lipoarabinomannan (ManLAM), lacks agonist activity. In contrast, PILAM, from Mycobacterum smegmatis, does have Our understanding of...
Magnetic resonance and ultrasound elastography techniques are now important tools for staging high-grade fibrosis in patients with chronic liver disease. However, uncertainty remains about the effects of simple accumulation fat (steatosis) inflammation (steatohepatitis) on parameters that can be measured using different elastographic techniques. To address this, we examine rheological models capable capturing dominant viscoelastic behaviors associated liver, quantify resulting changes shear...
Abstract Immunosenescence is a hallmark of aging and manifests as increased susceptibility to infection, autoimmunity, cancer in the elderly. One component immunosenescence thymic involution, age‐associated shrinkage thymus, observed all vertebrates studied date. The naked mole rat ( Heterocephalus glaber ) has become an attractive animal model research due its extreme longevity resistance disease. Here, we show that rats display no involution up 11 years age. Furthermore, found large...
- The clinicopathologic and prognostic significance of ARID1A mutation in esophageal adenocarcinoma (EAC) is unknown.- To determine the morphological correlates ARID1A-deficient EAC.- One hundred twenty cases primary EAC were evaluated for a predetermined set histologic features immunohistochemistry ARID1A, p53, MLH1 performed on EAC, as well adjacent Barrett esophagus esophagus-associated dysplasia, when feasible. Associations between categorical variables analyzed by Fisher exact test,...