A5065135570- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neural Engineering
- Nerve injury and regeneration
- Photoreceptor and optogenetics research
- Nuclear Receptors and Signaling
- 3D Printing in Biomedical Research
- Nicotinic Acetylcholine Receptors Study
- RNA regulation and disease
- RNA Research and Splicing
- Renal and related cancers
- Genetics, Aging, and Longevity in Model Organisms
- Nanoplatforms for cancer theranostics
- Developmental Biology and Gene Regulation
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- Parkinson's Disease Mechanisms and Treatments
- Biomedical Ethics and Regulation
- Congenital heart defects research
- Hereditary Neurological Disorders
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Neuroblastoma Research and Treatments
Memorial Sloan Kettering Cancer Center
2016-2025
Kettering University
2016-2025
Research Network (United States)
2024
Cornell University
2003-2024
Weill Cornell Medicine
2023-2024
New York Stem Cell Foundation
2016-2017
Institute for Stem Cell Biology and Regenerative Medicine
2015
New York Proton Center
2012
Goethe University Frankfurt
2010
Virginia Commonwealth University
2009
Human embryonic stem (hES) cells are defined by their extensive self-renewal capacity and potential to differentiate into any cell type of the human body. The challenge in using hES for developmental biology regenerative medicine has been direct wide differentiation toward derivation a specific fate. Within nervous system, have shown vitro neural progenitor cells, neurons, astrocytes. However, our knowledge, selective given neuron subtype not yet demonstrated. Here, we describe conditions...
Standard cell culture systems impose environmental oxygen (O(2)) levels of 20%, whereas actual tissue O(2) in both developing and adult brain are an order magnitude lower. To address whether proliferation differentiation CNS precursors vitro influenced by the environment, we analyzed embryonic day 12 rat mesencephalic precursor cells traditional cultures with 20% lowered (3 +/- 2%). Proliferation was promoted apoptosis reduced when were grown O(2), yielding greater numbers precursors. The...
Neural stem cells (NSCs) yield both neuronal and glial progeny, but their differentiation potential toward multiple region-specific neuron types remains remarkably poor. In contrast, embryonic cell (ESC) progeny readily fates in response to appropriate developmental signals. Here we demonstrate prospective clonal isolation of neural rosette (termed R-NSCs), a novel NSC type with broad CNS PNS capable vivo engraftment. R-NSCs can be derived from human mouse ESCs or plate stage embryos. While...
Embryonic stem (ES) cells are fully pluripotent in that they can differentiate into all cell types, including gametes. We have derived 35 ES lines via nuclear transfer (ntES lines) from adult mouse somatic of inbred, hybrid, and mutant strains. ntES contributed to an extensive variety dopaminergic serotonergic neurons vitro germ vivo. Cloning by nuclei could result normal development fertile adults. These studies demonstrate the full pluripotency cells.
Human embryonic stem cells provide access to the earliest stages of human development and may serve as a source specialized for regenerative medicine. Thus, it becomes crucial develop protocols directed differentiation into tissue-restricted precursors.Here, we present culture conditions derivation unlimited numbers pure mesenchymal precursors from demonstrate multilineage fat, cartilage, bone, skeletal muscle cells.Our findings will help elucidate mechanism mesoderm specification during...
Motoneurons represent a specialized class of neurons essential for the control body movement. Motoneuron loss is cause wide range neurological disorders including amyotrophic lateral sclerosis and spinal muscular atrophy. Embryonic stem cells are promising cell source study potential treatment motoneuron diseases. Here, we present novel in vitro protocol directed differentiation human embryonic (hESCs) into engraftable motoneurons. Neural induction hESCs was induced on MS5 stromal feeders,...
Parkinson's disease (PD) is an age-related neurodegenerative disorder characterized by the accumulation of protein aggregates comprised α-synuclein (α-syn). A major barrier in treatment discovery for PD lack identifiable therapeutic pathways capable reducing human neuronal model systems. Mutations key components trafficking and cellular degradation machinery represent important risk factors PD; however, their precise role progression interaction with α-syn remains unclear. Here, we find that...