A5023251236- Pancreatic function and diabetes
- Power Line Inspection Robots
- Robotics and Sensor-Based Localization
- CRISPR and Genetic Engineering
- Advanced Neural Network Applications
- Pluripotent Stem Cells Research
- Teleoperation and Haptic Systems
- Single-cell and spatial transcriptomics
- Diabetes and associated disorders
- RNA modifications and cancer
- Phagocytosis and Immune Regulation
- Congenital heart defects research
- Cancer, Hypoxia, and Metabolism
- SARS-CoV-2 and COVID-19 Research
- Cancer Mechanisms and Therapy
- Genetic Associations and Epidemiology
- Neonatal Respiratory Health Research
- Image Enhancement Techniques
- COVID-19 Clinical Research Studies
- Peptidase Inhibition and Analysis
- Elevator Systems and Control
- Advanced Vision and Imaging
- Neuroscience and Neural Engineering
- Vagus Nerve Stimulation Research
- Power Systems Fault Detection
Weill Cornell Medicine
2020-2024
Cornell University
2020-2024
National Institutes of Health
2024
National Heart Lung and Blood Institute
2024
State Grid Corporation of China (China)
2024
Genomic Health (United States)
2022-2023
Huainan Normal University
2019-2022
University of Hong Kong
2014-2020
Anhui University of Science and Technology
2020
University of Science and Technology of China
2020
Recent clinical data have suggested a correlation between coronavirus disease 2019 (COVID-19) and diabetes. Here, we describe the detection of SARS-CoV-2 viral antigen in pancreatic beta cells autopsy samples from individuals with COVID-19. Single-cell RNA sequencing immunostaining ex vivo infections confirmed that multiple types islet were susceptible to SARS-CoV-2, eliciting cellular stress response induction chemokines. Upon infection, showed lower expression insulin higher alpha acinar...
Summary Paragraph The SARS-CoV-2 virus has caused already over 3.5 million COVID-19 cases and 250,000 deaths globally. There is an urgent need to create novel models study using human disease-relevant cells understand key features of biology facilitate drug screening. As primary infection respiratory-based, we developed a lung organoid model pluripotent stem (hPSCs) that could be adapted for screens. organoids, particularly aveolar type II cells, express ACE2 are permissive infection....
Genetic studies have identified numerous loci associated with type 2 diabetes (T2D), but the functional roles of many remain unexplored. Here, we engineered isogenic knockout human embryonic stem cell lines for 20 genes T2D risk. We examined impacts each on β differentiation, functions, and survival. generated gene expression chromatin accessibility profiles cells derived from line. Analyses T2D-association signals overlapping HNF4A-dependent ATAC peaks a likely causal variant at FAIM2...
It is urgent to develop disease models dissect mechanisms regulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Here, we derive airway organoids from human pluripotent stem cells (hPSC-AOs). The hPSC-AOs, particularly ciliated-like cells, are permissive SARS-CoV-2 Using this platform, perform a high content screen and identify GW6471, which blocks GW6471 can also block infection of the B.1.351 variant. RNA sequencing (RNA-seq) analysis suggests that at least in...
Abstract COVID-19 is a systemic disease involving multiple organs. We previously established platform to derive organoids and cells from human pluripotent stem model SARS-CoV-2 infection perform drug screens 1,2 . This provided insight into cellular tropism the host response, yet molecular mechanisms regulating remain poorly defined. Here we systematically examined changes in transcript profiles caused by at different multiplicities of for lung airway organoids, alveolar cardiomyocytes,...
Background and Aims: Esophageal squamous cell carcinoma (ESCC), a major histologic subtype of esophageal cancer, is increasing in incidence, but the genetic underpinnings this disease remain unexplored. The aim study to identify recurrent changes, elucidate their roles discover new biomarkers for improving clinical management ESCC. Methods: Western blotting immunohistochemistry were performed detect expression level RHCG. Bisulfite genomic sequencing (BGS) methylation-specific PCR (MSP) used...
<title>Abstract</title> COVID-19 patients commonly present with neurological signs of central nervous system (CNS) and/or peripheral dysfunction. However, which neural cells are permissive to infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been controversial. Here, we show that midbrain dopamine (DA) neurons derived from human pluripotent stem (hPSCs) selectively SARS-CoV-2 both in vitro and upon transplantation vivo, triggers a DA neuron inflammatory cellular...
Reprogramming of intracellular metabolism is common in liver cancer cells. Understanding the mechanisms cell metabolic reprogramming may present a new basis for treatment. In our previous study, we reported that novel oncogene eukaryotic translation initiation factor 5A2 (EIF5A2) promotes tumorigenesis under hypoxic condition. Here, aim to investigate role EIF5A2 during hepatocellular carcinoma (HCC) development. this messenger RNA (mRNA) level was upregulated 59 105 (56.2%) HCC clinical...
Abstract Inflammatory factors and activation of oncogenes both played critical roles in the development progression human hepatocellular carcinoma (HCC). However, interplay between these two has not been well studied. In this study, we found that regulated by TNFα, Pim-2 proto-oncogene, serine/threonine kinase (PIM2) was highly expressed HCC correlated with poor prognosis ( P = 0.007) as tumor recurrence 0.014). Functional studies showed PIM2 could enhance abilities cell proliferation,...
Abstract Maintenance tasks in distribution networks are often accompanied by hazards associated with high altitudes and voltages. By utilising robots instead of human operators to perform these tasks, potential risks can be avoided, while productivity increased. This research proposes an intelligent power live‐line operation robot (PDLOR) system based on a stereo camera replace complete work. The PDLOR consists several key components, including dual manipulators, wireless tools, visual...
Abstract Genome wide association studies (GWAS) have identified over 100 signals associated with type 1 diabetes (T1D). However, translating any given T1D GWAS signal into mechanistic insights, such as causal variants, their target genes, and the specific cell types involved, has been challenging. Here, we present a comprehensive multi-omic integrative analysis of single-cell/nucleus resolution profiles gene expression chromatin accessibility in healthy autoantibody+ (AAB+) human pancreatic...
Abstract The inspection and maintenance of power distribution network are crucial for efficiently delivering electricity to consumers. Due the high voltage lines, manual live-line operations difficult, risky, inefficient. This paper researches a Power Distribution Network Live-line Operation Robot (PDLOR) with autonomous tool assembly capabilities replace humans in various high-risk electrical tasks. To address challenges dynamic unstructured work environments PDLOR, we propose framework...
Genetic association studies have identified hundreds of independent signals associated with type 2 diabetes (T2D) and related traits. Despite these successes, the identification specific causal variants underlying a genetic signal remains challenging. In this study, we describe deep learning (DL) method to analyze impact sequence on enhancers. Focusing pancreatic islets, T2D relevant tissue, show that our model learns islet-specific transcription factor (TF) regulatory patterns can be used...
Study of disease-relevant immune cells, namely monocytes and macrophages, is limited based on availability primary tissue, a limitation that can be remedied using human induced pluripotent stem cell (hiPSC) technology. Here, we present protocol for differentiation macrophages from hiPSCs. We describe steps hiPSC maintenance, mesoderm lineage induction, hematopoietic progenitor cells (HPCs) commitment expansion, myeloid induction. then detail procedures monocyte formation functional...
Teleoperation system has been an indispensable part of the research on live power line maintaining robots. In this paper, we propose a teleoperation combining direct haptic feedback (DHF) with graphical force (GFF), which effectively improves sensitivity feedback, control accuracy and efficiency maintenance tasks. We create place suitably color progress bar for operator to experience graphically, build using mathematical methods practical perspectives. Five participators are invited test...
It takes a huge amount of works to take pictures transmission line towers and check electrical fittings manually. In spite the introduction deep learning technology inspection, it is not well utilized that fine-grained contexts on components in state-of-the-art research. On basis region-based fully convolutional network (R-FCN), novel object detection method proposed considering among fittings. Deformable convolution layers squeeze-and-excitation (SE) blocks are adopted method. A comparison...
Abstract Kif5b-driven anterograde transport and clathrin-mediated endocytosis (CME) are responsible for opposite intracellular trafficking, contributing to plasma membrane homeostasis. However, whether how the two trafficking processes coordinate remain unclear. Here, we show that Kif5b directly interacts with clathrin heavy chain (CHC) at a region close uncoating catalyst (Hsc70) preferentially localizes on relatively large clathrin-coated vesicles (CCVs). Uncoating in vitro is decreased...
In order to avoid the accident of manual live operation, make working operation safer and improve efficiency, it is general trend use robot or human-machine combination for operation. When operated remotely by operator, detailed information scene often not available, a large amount three-dimensional lost due monitoring screen, leading operator's poor sense presence immersion, more hidden dangers. view above problems, this paper designs virtual reality device that combines binocular camera...
Abstract Type 1 diabetes (T1D) is a complex autoimmune disease characterized by the loss of pancreatic islet beta cells. The mechanisms T1D genetic risk remain poorly understood. Here, we present multi-omic integrative study single-cell/nucleus molecular profiles gene expression and chromatin accessibility in same biological samples from healthy cell autoantibody + (AAB+) human islets to characterize islet-mediated risk. We additionally performed profiling under two stimulatory conditions...