A5089224813- Autophagy in Disease and Therapy
- Extracellular vesicles in disease
- Mitochondrial Function and Pathology
- Circadian rhythm and melatonin
- Nanoparticles: synthesis and applications
- Adipose Tissue and Metabolism
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Phagocytosis and Immune Regulation
- Salmonella and Campylobacter epidemiology
- Cell death mechanisms and regulation
- Muscle Physiology and Disorders
- Microplastics and Plastic Pollution
- Erythrocyte Function and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
- Vitamin C and Antioxidants Research
- Dendrimers and Hyperbranched Polymers
- MicroRNA in disease regulation
- Aquaculture disease management and microbiota
- Cholesterol and Lipid Metabolism
- Environmental Toxicology and Ecotoxicology
- T-cell and B-cell Immunology
- Adipokines, Inflammation, and Metabolic Diseases
- Graphene and Nanomaterials Applications
- Vibrio bacteria research studies
University of Urbino
2016-2025
Ambiente Italia (Italy)
2010
Morpho (United States)
2006
University of Pavia
2006
University of Milan
2006
Ospedale Maggiore
2006
Istituti di Ricovero e Cura a Carattere Scientifico
2006
In the past few years, skeletal muscle has emerged as an important secretory organ producing soluble factors, called myokines, that exert either autocrine, paracrine or endocrine effects. Moreover, recent studies have shown releases microRNAs into bloodstream in response to physical exercise. These affect target cells, such hormones and cytokines. The mechanisms underlying microRNA secretion are poorly characterized at present. Here, we investigated whether tissue extracellular vesicles...
The potential toxicity of engineered nanoparticles (NPs) for humans and the environment represents an emerging issue. Since aquatic ultimate sink NP deposition, development suitable assays is needed to evaluate impact NPs on biota. immune system a sensitive target NPs, conservation innate immunity useful basis studying common biological responses NPs. Suspension-feeding invertebrates, such as bivalves, are particularly at risk exposure, since they have extremely developed systems uptake nano...
Abstract Mitochondrial dysfunction is considered one of the hallmarks ischemia/reperfusion injury. Mitochondria are plastic organelles that undergo continuous biogenesis, fusion, and fission. They can be transferred between cells through tunneling nanotubes (TNTs), dynamic structures allow exchange proteins, soluble molecules, organelles. Maintaining mitochondrial dynamics crucial to cell function survival. The present study aimed assess effects melatonin on dynamics, TNT formation,...
Bivalves are widespread in coastal environments subjected to a wide range of environmental fluctuations: however, the rapidly occurring changes due several anthropogenic factors can represent significant threat bivalve immunity. The mussel Mytilus spp. has extremely powerful immune defenses toward different potential pathogens and contaminant stressors. In particular, system represents target for types nanoparticles (NPs), including amino-modified nanopolystyrene (PS-NH2) as model...
Exercise-released extracellular vesicles (EVs) are emerging as a novel class of exerkines that promotes systemic beneficial effects. However, slight differences in the applied exercise protocols terms mode, intensity and duration, well need for standardized EV isolation, make comparison studies literature extremely difficult. This work aims to investigate amount EV-associated miRNAs released circulation response different physical regimens. Healthy individuals were subjected protocols: acute...
Extracellular vesicles (EVs) play a crucial role in the intercellular crosstalk. Mesenchymal stem cell-derived EVs (MSC-EVs), displaying promising therapeutic roles, contribute to strong rationale for developing as an alternative option. EV analysis still represents one of major issues be solved order translate use MSC-EV detection clinical settings. Even if flow cytometry (FC) has been largely applied studies, lack consensus on protocols FC generated controversy. Standard procedures, based...
Phenylketonuria (PKU) is an autosomal recessive genetic disease caused by defects in the phenylalanine hydroxylase gene. Preclinical and clinical investigations suggest that ammonia lyase (PAL) could be effective alternative for treatment of PKU. The aim this study to investigate if erythrocytes loaded with PAL may act as a safe delivery system able overcome bioavailability issues provide, vivo, therapeutically relevant concentration enzyme. Murine were recombinant from Anabaena variabilis...
Cancer chemotherapy is characterized by an elevated intrinsic toxicity and the development of drug resistance. Thus, there a compelling need for new intervention strategies with improved therapeutic profile. Immunogenic cell death (ICD) represents innovative anticancer strategy where dying cancer cells release damage-associated molecular patterns promoting tumor-specific immune responses. The roots Withania somnifera (W. somnifera) are used in Indian traditional medicine their...
Niemann-Pick disease type A (NP-A) and B (NP-B) are lysosomal storage diseases (LSDs) caused by sphingomyelin accumulation in lysosomes relying on reduced or absent acid sphingomyelinase. considerable body of evidence suggests that many LSD impairs autophagy, resulting the poly-ubiquitinated proteins dysfunctional mitochondria, ultimately leading to cell death. Here we test this hypothesis a cellular model B, which autophagy has never been studied. The basal autophagic pathway was first...
Abstract Extracellular vesicles (EVs) are promising natural nanocarriers for the delivery of therapeutic agents. As with any other kind cell, red blood cells (RBCs) produce a limited number EVs under physiological and pathological conditions. Thus, RBC-derived extracellular (RBCEVs) have been recently suggested as next-generation systems purposes. In this paper, we show that thanks to their unique biological physicochemical features, RBCs can be efficiently pre-loaded several kinds molecules...
Abstract: Melatonin influences circadian rhythms and acts as antioxidant free radical scavenger. UV irradiation triggers multiple cellular events which lead to cell death, in particular apoptosis; this process involves reactive oxygen species. Apoptotic machinery several pathways, mitochondria play crucial roles. In work we have evaluated by means of cytometric, biochemical ultrastructural approaches, if incubation U937 promonocytic leukemia cells with melatonin may affect apoptotic behavior...
Several studies indicate that plasma membrane changes during apoptosis are a general phenomenon. Among the flow cytometric methods to measure apoptosis, Annexin V assay detects exposure of phosphatidylserine (PS) is one most commonly used. However, various treatments used for detachment adherent cells generally interfere with binding PS, making measurement technical problem. Materials and Methods Apoptosis different cell lines was investigated by fluorescence microscopy multiple assays...