A5107463113- Cell death mechanisms and regulation
- Muscle Physiology and Disorders
- RNA Interference and Gene Delivery
- Acute Lymphoblastic Leukemia research
- Mitochondrial Function and Pathology
- Osteoarthritis Treatment and Mechanisms
- Nuclear Structure and Function
- Autophagy in Disease and Therapy
- Exercise and Physiological Responses
- Phagocytosis and Immune Regulation
- Chronic Lymphocytic Leukemia Research
- Tendon Structure and Treatment
- Circadian rhythm and melatonin
- Biochemical and Molecular Research
- Erythrocyte Function and Pathophysiology
- PI3K/AKT/mTOR signaling in cancer
- Muscle activation and electromyography studies
- DNA Repair Mechanisms
- Shoulder Injury and Treatment
- DNA and Nucleic Acid Chemistry
- Sports injuries and prevention
- Inflammatory mediators and NSAID effects
- Sports Performance and Training
- Viral gastroenteritis research and epidemiology
- Phytochemicals and Antioxidant Activities
University of Urbino
2013-2022
Istituto Ortopedico Rizzoli
2006-2016
University of Padua
2015
Istituto di Genetica Molecolare
2008-2014
Institute of Molecular Genetics
2013-2014
National Research Council
2011-2013
Orthopedic Institute
2012
University of Bologna
1983-2010
East Carolina University
2010
Policlinico S.Orsola-Malpighi
1983-2010
Recent findings have highlighted that constitutively active phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling is a common feature T-cell acute lymphoblastic leukemia (T-ALL), where it upregulates cell proliferation, survival, and drug resistance. These observations lend compelling weight to the application PI3K/Akt/mTOR inhibitors in therapy T-ALL. Here, we analyzed therapeutic potential novel dual PI3K/mTOR inhibitor NVP-BEZ235, an orally bioavailable...
By flow cytometry, we have quantitatively evaluated HL-60, MOLT-4, and P815 apoptotic cells induced with camptothecin, staurosporine, hyperthermia, respectively. Apoptosis was measured by two different cytometry techniques, using propidium iodide (PI) uptake in permeabilized nonpermeabilized cells. also has been analyzed electron microscopy DNA cleavage situ nick translation gel electrophoresis. We demonstrate that supravital exposure to without prior permeabilization identifies cells,...
Abstract Flow cytometry has recently become a choice technique for the quantitative analysis of apoptosis. Monoparametric DNA usually allows identification apoptotic cells as “subdiploid” peak. Progression through apoptosis leads to chromatin condensation, nuclear fragmentation and eventually cell disruption. Thus, major problem flow cytometric populations is discrimination between debris cells. Here we demonstrate that best parameter on which make such distinction content, no matter what...
Abstract It is known that the MyoD family members (MyoD, Myf5, myogenin, and MRF4) play a pivotal role in complex mechanism of skeletal muscle cell differentiation. However, fragmentary information on transcription factor‐specific regulation available data their post‐transcriptional post‐translational behavior are still missing. In this work, we combined mRNA protein expression analysis with subcellular localization. Each myogenic regulator factor (MRF) revealed specific trend quantitative...
The human meniscus plays a crucial role for transmission and distribution of load across the knee, as well shock absorption, joint stability, lubrication, congruity. aim this study was to compare complex geometry, unique ultrastructure tissue composition in healthy (control) pathological conditions provide understanding structural changes that could be helpful future design targetted therapies improvement treatment indications. We analyzed samples collected from 3 multi-organ donors (median...
Abstract Creatine (Cr), one of the most popular nutritional supplements among athletes, has been recently shown to prevent cytotoxicity caused by different oxidative stressors in various mammalian cell lines, including C2C12 myoblasts, via a direct antioxidant activity. Here, effect Cr on differentiating capacity cells exposed H 2 O investigated. Differentiation into myotubes was monitored using morphological, ultrastructural, and molecular techniques. Treatment with (1 h) not only...
This study describes mitochondrial behaviour during the C2C12 myoblast differentiation program and proposes a proteomic approach to mitochondria integrated with classical morphofunctional biochemical analyses. Mitochondrial ultrastructure variations were determined by transmission electron microscopy; mass membrane potential analysed Mitotracker Green JC-1 stains epifluorescence microscope. Expression of PGC1α, NRF1α, Tfam genes controlling biogenesis was studied real-time PCR. The...
Ultraviolet B (UVB) radiation acts as a strong apoptotic trigger in many cell types, tumor and normal cells. Several studies have demonstrated that UVB-induced death occurs through the generation of reactive oxygen species. The consequent oxidative stress includes impairment cellular antioxidants, induction DNA damage occurrence apoptosis. In this review, we investigated UVB action various models by using ultrastructural, molecular cytofluorimetric techniques. Myeloid leukemia HL-60,...
Longicyatholaimus maldivarum was a species erected by Gerlach in 1964 from specimens found the Maldivian archipelago. Due to finding of additional representatives this species, L. is re-described with addition new morphological details mainly about males, because information female original description are very scarce. The newly showed total body length higher than type (1936–2063 µm vs. 1330 long) as well ratio divided maximum diameter (22–28 27). multispiral amphideal fovea 5 turns 4 and...