A5071989994- Osteoarthritis Treatment and Mechanisms
- Tendon Structure and Treatment
- Lower Extremity Biomechanics and Pathologies
- Knee injuries and reconstruction techniques
- Parkinson's Disease Mechanisms and Treatments
- Musculoskeletal synovial abnormalities and treatments
- Shoulder Injury and Treatment
- Total Knee Arthroplasty Outcomes
- Shoulder and Clavicle Injuries
- Cellular transport and secretion
- Orthopedic Surgery and Rehabilitation
- Immune cells in cancer
- Foot and Ankle Surgery
- Hip disorders and treatments
- Trauma Management and Diagnosis
- Immune Response and Inflammation
- Rheumatoid Arthritis Research and Therapies
- Bone fractures and treatments
- Diabetic Foot Ulcer Assessment and Management
- Bone Tumor Diagnosis and Treatments
- Bone and Joint Diseases
- Mitochondrial Function and Pathology
- Neuroscience and Neuropharmacology Research
- Reproductive System and Pregnancy
- Chemokine receptors and signaling
University of Padua
2016-2025
The infrapatellar fat pad (IFP) is considered a local producer of adipocytokines, suggesting potential role in OA. objective this study was to evaluate the histopathological and molecular characteristics OA IFPs compared with controls. were evaluated patients undergoing total knee replacements control (without OA), considering following parameters: presence inflammatory cells, vascularization, adipose lobules dimension thickness interlobular septa. Immunohistochemistry performed VEGF,...
Lrrk2, a gene linked to Parkinson's disease, encodes large scaffolding protein with kinase and GTPase activities implicated in vesicle cytoskeletal-related processes. At the presynaptic site, LRRK2 associates synaptic vesicles through interaction panel of proteins. Here, we show that activity influences dynamics fusion. We therefore investigated whether phosphorylates component(s) exo/endocytosis machinery. have previously observed interacts NSF, hexameric AAA+ ATPase couples ATP hydrolysis...
Mutations in Leucine-rich repeat kinase 2 gene (LRRK2) are associated with familial and sporadic Parkinson's disease (PD). LRRK2 is a complex protein that consists of multiple domains executing several functions, including GTP hydrolysis, activity binding. Robust evidence suggests acts at the synaptic site as molecular hub connecting vesicles to cytoskeletal elements via panel protein-protein interactions. Here we investigated impact pharmacological inhibition on function. Acute treatment...
Osteoarthritis (OA) is one of the most common joint disorders. Evidence suggests that infrapatellar fat pad (IFP) directly involved in OA pathology. However, a comparison between versus non-OA IFP still missing. Thus, aim this study was to compare molecular, adipocytes and extracellular matrix characteristics patients affected by OA, undergoing anterior cruciate ligament (ACL) reconstruction. We hypothesized not only inflammation but also changes (ECM) composition might be pathogenesis....
The Infrapatellar Fat Pad (IFP) is a fibro-adipose tissue of the knee recently reconsidered as part single anatomo-functional unit (AFU) together with synovial membrane (SM). Several evidence support role this in mechanisms that trigger and perpetuate onset progression osteoarthritis (OA) disease. Additionally, contribution IFP-SM AFU OA-associated pain has also been supposed, but assumption still needs to be fully elucidated. Within context, recent discovery mechanoceptive Piezo ion...
Articular cartilage is a complex connective tissue with the fundamental functions of load bearing, shock absorption and lubrication in joints. However, traumatic events, aging degenerative pathologies may affect its structural integrity function, causing pain long-term disability. Osteoarthritis represents health issue, which concerns an increasing number people worldwide. Moreover, it has been observed that this pathology also affects mechanical behavior articular cartilage. To better...
Abstract Background Hallux valgus (HV) is a widespread condition that leads to discomfort in daily life. There are different surgical techniques for HV. This retrospective and comparative study aimed compare the clinical radiographic outcomes of Reverdin-Isham osteotomy (RIO) Minimally Invasive Intramedullary Nail Device (MIIND) techniques. Methods One hundred ninety-six patients with mild-to-severe HV were enrolled divided into two groups: 98 mild-moderate moderate-severe HV, treated RIO...
Leucine-rich repeat kinase 1 and 2 (LRRK1 LRRK2) are large multidomain proteins containing kinase, GTPase multiple protein-protein interaction domains, but only mutations in LRRK2 linked to familial Parkinson's disease (PD). Independent studies suggest that exists the cell as a complex compatible with size of dimer. However, whether this is truly homodimer or heterologous formed by monomeric other has not been definitively proven due limitations obtaining highly pure suitable for structural...
Abstract The aim of this study was to identify the molecules and pathways involved in cross‐talk between meniscus synovium that may play a critical role osteoarthritis (OA) pathophysiology. Samples were collected from patients with early end‐stage OA cultured alone or cocultured. Cytokines, chemokines, metalloproteases, their inhibitors evaluated at gene protein levels. extracellular matrix (ECM) changes also investigated. In cultures, higher levels interleukin‐6 (IL‐6) IL‐8 messenger RNA...
Familial and idiopathic Parkinson's disease (PD) is associated with the abnormal neuronal accumulation of α-synuclein (aS) leading to β-sheet-rich aggregates called Lewy Bodies (LBs). Moreover, single point mutation in aS gene multiplication lead autosomal dominant forms PD. A connection between PD 14-3-3 chaperone-like proteins was recently proposed, based on fact that some isoforms can interact genetic PD-associated such as parkin, LRRK2 were found components LBs human In particular, a...
Leucine-rich repeat kinase 2 (LRRK2) is a causative gene for Parkinson's disease, but the physiological function and mechanism(s) by which cellular activity of LRRK2 regulated are poorly understood. Here, we identified p21-activated 6 (PAK6) as novel interactor GTPase/ROC domain LRRK2. kinases serine-threonine that serve targets small GTP binding proteins Cdc42 Rac1 have been implicated in different morphogenetic processes through remodeling actin cytoskeleton such synapse formation...
Aim: Osteoarthritis (OA) is a whole joint pathology involving cartilage, synovial membrane, meniscus, subchondral bone, and infrapatellar fat pad (IFP). Synovitis has been widely documented in OA suggesting its important role pathogenesis. The aim of this study was to investigate the different tissues promoting synovitis. Materials methods: Conditioned media (CM) from IFP were generated five patients undergoing total knee replacement used stimulate human fibroblast-like synoviocytes cell...
The human meniscus plays a crucial role for transmission and distribution of load across the knee, as well shock absorption, joint stability, lubrication, congruity. aim this study was to compare complex geometry, unique ultrastructure tissue composition in healthy (control) pathological conditions provide understanding structural changes that could be helpful future design targetted therapies improvement treatment indications. We analyzed samples collected from 3 multi-organ donors (median...
Several studies have investigated cartilage degeneration and inflammatory subchondral bone synovial membrane changes using magnetic resonance (MR) in osteoarthritis (OA) patients. Conversely, there is a paucity of data exploring the role knee ligaments, infrapatellar fat pad (IFP), suprapatellar (SFP) OA compared to post-traumatic cohorts Therefore, aim this study was analyze volumetric morphometric characteristics following joint tissues: IFP (volume, surface, depth, femoral tibial arch...
Mitochondrial dysfunction and oxidative stress are considered central in dopaminergic neurodegeneration Parkinson's disease (PD). Oxidative occurs when the endogenous antioxidant systems overcome by generation of reactive oxygen species (ROS). A plausible source stress, which could account for selective degeneration neurons, is redox chemistry dopamine (DA) leads to formation ROS dopamine-quinones (DAQs). Superoxide dismutase 2 (SOD2) a mitochondrial enzyme that converts superoxide radicals...
Recently, infrapatellar fat pad (IFP) has been considered as a source of stem cells for cartilage regeneration in osteoarthritis (OA) due to their ability differentiation into chondrocytes. However, stressful conditions, like that related OA, may induce pathogenic reprograming. The aim this study was characterize the structural and functional properties new population isolated from osteoarthritic (OA-IFP). Nine OA patients undergoing total knee arthroplasty (TKA) were enrolled [median age =...
Abstract Polyglutamine expansion in the androgen receptor (AR) causes spinobulbar muscular atrophy (SBMA). Skeletal muscle is a primary site of toxicity; however, current understanding early pathological processes that occur and how they unfold during disease progression remains limited. Using transgenic knock-in mice patient-derived biopsies, we show SBMA presymptomatic stage develop respiratory defect matching defective expression genes involved excitation-contraction coupling (ECC),...
Because chondrosarcomas vary widely in their behavior, and because anticipating behavior based on histology alone can be challenging, genetic markers represent an appealing area of inquiry that may help us refine our prognostic approaches. Isocitrate dehydrogenase (IDH) mutations are involved the pathogenesis a variety neoplasms, recently, IDH1/2 have been found tissue benign cartilage tumors as well conventional highly aggressive dedifferentiated chondrosarcomas. However, association with...
Genetic studies show that LRRK2, and not its closest paralogue LRRK1, is linked to Parkinson's disease. To gain insight into the molecular cellular basis of this discrepancy, we searched for LRRK1- LRRK2-specific processes by identifying their distinct interacting proteins. A protein microarray-based interaction screen was performed with recombinant 3xFlag-LRRK1 3xFlag-LRRK2 and, in parallel, co-immunoprecipitation followed mass spectrometry from SH-SY5Y neuroblastoma cell lines stably...