A5088919162- Extracellular vesicles in disease
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Tissue Engineering and Regenerative Medicine
- Protein Kinase Regulation and GTPase Signaling
- Angiogenesis and VEGF in Cancer
- Retinoids in leukemia and cellular processes
- Erythrocyte Function and Pathophysiology
- Cell Adhesion Molecules Research
- Platelet Disorders and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Inflammatory Biomarkers in Disease Prognosis
- HIV Research and Treatment
- Cell death mechanisms and regulation
- Cellular transport and secretion
- RNA Interference and Gene Delivery
- Galectins and Cancer Biology
- Chronic Lymphocytic Leukemia Research
- NF-κB Signaling Pathways
- Reproductive Biology and Fertility
- Corneal Surgery and Treatments
- Wnt/β-catenin signaling in development and cancer
- Cytokine Signaling Pathways and Interactions
- Acute Myeloid Leukemia Research
- Immune Response and Inflammation
University of Chieti-Pescara
2014-2023
Yale University
2020
Harvard University
2020
Brigham and Women's Hospital
2020
Federation of American Societies for Experimental Biology
2020
Rockefeller University
2020
National Institutes of Health
2020
University Foundation
2014-2015
Centre for Excellence in Mining Innovation
2015
Foundation University Islamabad
2003-2014
The prevalence and clinical picture of hypertensive urgencies emergencies in an emergency department are poorly known. aim the present study was to evaluate crises (urgencies emergencies) during 12 months observation frequency end-organ damage with related pictures first 24 hours after presentation. Hypertensive (76% urgencies, 24% represented more than one fourth all medical urgencies-emergencies. most frequent signs presentation were headache (22%), epistaxis (17%), faintness, psychomotor...
Multiple Sclerosis (MuS) is a complex multifactorial neuropathology, resulting in heterogeneous clinical presentation. A very active MuS research field concerns the discovery of biomarkers helpful to make an early and definite diagnosis. The sphingomyelin pathway has emerged as molecular mechanism involved MuS, since high levels ceramides cerebrospinal fluid (CSF) were related axonal damage neuronal dysfunction. Ceramides are hydrolysis products sphingomyelins through reaction catalyzed by...
Regulatory T Cells (Tregs) are a T-lymphocyte subset involved in the maintenance of immune peripheral tolerance. Despite evidence adaptive system's role Alzheimer's Disease (AD), involvement Tregs is still not clear. We focused on Flow-Cytometry analysis Treg frequencies and phenotypes AD. The aim study to analyse similarities differences profile between Multiple Sclerosis. (CD4+/CD25high/CD127low-neg) were identified using an innovative Flow Cytometry method subtyped as Resting (analysed...
Molecular markers for prostate cancer (PCa) are required to improve the early definition of patient outcomes. Atypically large extracellular vesicles (EVs), referred as "Large Oncosomes" (LO), have been identified in highly migratory and invasive PCa cells. We recently developed characterized DU145R80 subline, selected from parental DU145 cells resistant inhibitors mevalonate pathway. showed different proteomic profile compared cells, along with altered cytoskeleton dynamics a more...
Abstract Manipulation of stem cells or cells-derived secretome has emerged as a novel alternative therapeutic option for multiple sclerosis (MS). Here we show that human periodontal ligament (hPDLSCs)-derived conditioned medium (hPDLSCs-CM) and purified exosomes/microvesicles (hPDLSCs-EMVs) obtained from Relapsing Remitting (RR)-MS patients healthy donors block experimental autoimmune encephalomyelitis (EAE), mouse model MS, by inducing anti-inflammatory immunosuppressive effects in spinal...
Unresolved inflammation and tissue destruction are underlying mechanisms of periodontitis, which is linked to dysregulated polymorphonuclear neutrophil (PMN) functions. Lipoxin A4 (LXA4) a specialized proresolving lipid mediator (SPM) that dampens excessive inflammation, promotes resolution, protects from leukocyte-mediated damage. Human periodontal ligament stem cells (hPDLSCs) represent key players during regeneration may contribute resolution inflammation; thus, they promising tool in...
Human amniotic fluid mesenchymal stem cells (huAFMSCs) are emerging as a promising therapeutic option in regenerative medicine. Here, we characterized huAFMSC phenotype and multipotentiality. When cultured osteogenic medium, displayed significant increase in: Alkaline Phosphatase (ALP) activity mRNA expression, Alizarin Red S staining Runx2 expression; whereas maintaining these an adipogenic culture medium gave time-dependent PPARγ FABP4 glycerol-3-phosphate dehydrogenase (GPDH) positivity...
Background Many adult tissues contain a population of stem cells with the ability to regenerate structures similar microenvironments from which they are derived in vivo and represent promising therapy for regeneration complex clinical disorder. Human (SCs) including bone marrow (BMSCs), dental pulp (DPSCs) periodontal ligament (PDLSCs) have been characterized their high proliferative potential, expression characteristic SC-associated markers plasticity differentiate different lineage vitro....
Amniotic epithelial cells (AECs) are ideal seed for tissue regeneration, but no research has yet been reported on their tendon regeneration potential. This study investigated the efficiency of AEC allotransplantation healing, as well mechanism involved. To this aim ovine AECs, characterized by specific surface and stemness markers (CD14 - , CD31 CD45 CD49f, CD29, CD166, OCT4, SOX2, NANOG, TERT), were allotransplanted into experimentally induced defects in sheep Achilles tendon. In situ...
Background . Neural crest-derived mesenchymal stem cells (MSCs) from human oral tissues possess immunomodulatory and regenerative properties are emerging as a potential therapeutic tool to treat diverse diseases, such multiple sclerosis, myocardial infarction, connective tissue damages. In addition cell-surface antigens, dental MSCs express embryonic cell markers neural crest originate the ectoderm layer. vitro passages may eventually modify these marker expressions other stemness...
Primary open-angle glaucoma (POAG) represents the leading cause of irreversible blindness worldwide and is a multifactorial, chronic neurodegenerative disease characterized by retinal ganglion cell visual field loss. There are many factors that associated with risk developing POAG, increased intraocular pressure being one most prevalent. Due to asymptomatic nature disease, diagnosis POAG often occurs too late, which necessitates development new effective screening strategies for early...
Human Gingival Mesenchymal Stem Cells (hGMSC) are multipotential cells that can expand and differentiate in culture under specific standardized conditions. In the present study, we have investigated whether vitro pre-treatment of hGMCs with Cannabidiol (CBD) influence their expression profile, improving therapeutic potential this cell culture. Following CBD treatment (5μM) for 24 h, gene analysis through Next Generation Sequencing (NGS) has revealed several genes differentially expressed...
Extracellular vesicles (EVs) are released by shedding during different physiological processes and increasingly thought to be new potential biomarkers. However, the impact of pre-analytical processing phases on final measurement is not predictable for this reason, translation basic research into clinical practice has been precluded. Here we have optimized a simple procedure in combination with polychromatic flow cytometry (PFC), identify, classify, enumerate, separate circulating EVs from...
Rationale. Obesity is a risk factor for atherothrombosis and various cancers. However, the mechanisms are not yet completely clarified. Objectives. We aimed to verify whether microparticles (MPs) released from thrombin-activated platelets differed in obese nonobese women number, size, proteomics cargo capacity modulate vitro expression of (i) genes related epithelial mesenchymal transition (EMT) endothelial (EndMT), (ii) cyclooxygenase(COX)-2 involved production angiogenic inflammatory...
Platelet-cancer cell interactions modulate tumor metastasis and thrombosis in cancer. Platelet-derived extracellular vesicles (EVs) can contribute to these outcomes.We characterized the medium-sized EVs (mEVs) released by thrombin-stimulated platelets of colorectal cancer (CRC) patients healthy subjects (HS) on capacity induce epithelial-mesenchymal transition (EMT)-related genes cyclooxygenase (COX)-2(PTGS2), thromboxane (TX)B2 production cocultures with four lines. mEVs were assessed for...
We and others have previously demonstrated the existence of an autonomous nuclear polyphosphoinositide cycle that generates second messengers such as diacylglycerol (DAG), capable attracting to nucleus specific protein kinase C (PKC) isoforms (Neri et al. (1998) J. Biol. Chem. 273, 29738–29744). Recently, however, nuclei also been shown contain enzymes responsible for synthesis non-canonical 3-phosphorylated inositides. To clarify a possible role this peculiar class inositol lipids we...
Abstract Mesenchymal stem cells (MSCs) are self‐renewing with the ability to differentiate into various mesodermal‐derived tissues. Recently, we have identified in adult human periodontal ligament (PDL) a population of (PDL‐MSCs) osteoblasts and adipocytes. The aim present work was further characterize this expression profile its cells. To achieve our objective used flow cytometry, magnetic cell sorting, cytokine antibody array, light electron microscope immunostaining. Our results show that...