A5073243870- Prostate Cancer Treatment and Research
- Cystic Fibrosis Research Advances
- Inflammatory mediators and NSAID effects
- Eicosanoids and Hypertension Pharmacology
- Prostate Cancer Diagnosis and Treatment
- Neonatal Respiratory Health Research
- Fatty Acid Research and Health
- Hormonal and reproductive studies
- Immune Response and Inflammation
- Hemoglobin structure and function
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Peroxisome Proliferator-Activated Receptors
- Ultrasound and Hyperthermia Applications
- Forest Ecology and Biodiversity Studies
- S100 Proteins and Annexins
- Erythrocyte Function and Pathophysiology
- Neonatal Health and Biochemistry
- Asthma and respiratory diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Platelet Disorders and Treatments
- interferon and immune responses
- Diptera species taxonomy and behavior
- Mesenchymal stem cell research
- Forest Insect Ecology and Management
- Helicobacter pylori-related gastroenterology studies
University of Chieti-Pescara
2016-2025
Shenzhen Maternity and Child Healthcare Hospital
2024
Harvard University Press
2024
University of California, Davis
2024
Guangzhou Women and Children Medical Center
2024
Guangzhou Medical University
2024
Southern Medical University
2024
Peking University Shenzhen Hospital
2024
University of Alabama at Birmingham
2023
Stanford University
2023
The function of urokinase and its receptor is essential for cell migration in pathological conditions, as shown by the analysis knockout mice phenotypes. How a protease fibrinolytic pathway can induce not understood no link between this migration-promoting G protein-coupled receptors has been described. We now show that FPRL1/LXA4R, number polypeptides endogenous lipoxin A4 (LXA4), urokinase-type plasminogen activator (uPA) it directly interacts with an activated, soluble, cleaved form uPA...
Eicosanoids derived from lipoxygenase (LO)-catalyzed reactions play important roles in pulmonary inflammation. Here, we examined formation of LO-derived products by human alveolar macrophages (HAM). HAM converted [1-14C]-arachidonic acid to a product carrying 14C-radiolabel that was identified as 15(S)-hydroxy-5,8,11-cis-13-trans-eicosatetraenoic (15-HETE) physical methods. 15-LO mRNA demonstrated reverse transcription-polymerase chain reaction. Incubation for 3 d with interleukin 4(IL-4)...
Pseudomonas aeruginosa lung infection is a main cause of disability and mortality worldwide. Acute inflammation its timely resolution are crucial for ensuring bacterial clearance limiting tissue damage. Here, we investigated protective actions resolvin (Rv) D1 in induced by the RP73 clinical strain P. aeruginosa. RvD1 significantly diminished growth neutrophil infiltration during acute pneumonia caused RP73. Inoculum RP73, immobilized agar beads, resulted persistent up to 21 days, leading...
Unresolved inflammation and tissue destruction are underlying mechanisms of periodontitis, which is linked to dysregulated polymorphonuclear neutrophil (PMN) functions. Lipoxin A4 (LXA4) a specialized proresolving lipid mediator (SPM) that dampens excessive inflammation, promotes resolution, protects from leukocyte-mediated damage. Human periodontal ligament stem cells (hPDLSCs) represent key players during regeneration may contribute resolution inflammation; thus, they promising tool in...
Background —Hypercholesterolemia is considered a major risk factor for the development of atherosclerosis. Enhanced lipid peroxidation and persistent platelet activation can be observed in vivo hypercholesterolemic patients may have pathophysiological implications occurrence cardiovascular events. P-selectin play an important role pathogenesis multicellular events, including We studied impact hypercholesterolemia oxidative stress on plasma levels P-selectin. Methods Results —Plasma were...
Evidence indicates that lipoxygenases (LO) may play a role in cancer cell survival. We show human malignant pleural mesothelial (MM) cells, but not normal (NM) express catalytically active 5-LO. Pharmacological or genetic inhibition of MM 5-LO determined nucleosome formation and induced DNA fragmentation pattern typical apoptosis. This was completely reversed by exogenously added 5(S)-HETE 12(S)-, 15(S)-HETE, leukotriene (LT)B4. A antisense oligonucleotide potently time-dependently reduced...
Human amniotic fluid mesenchymal stem cells (huAFMSCs) are emerging as a promising therapeutic option in regenerative medicine. Here, we characterized huAFMSC phenotype and multipotentiality. When cultured osteogenic medium, displayed significant increase in: Alkaline Phosphatase (ALP) activity mRNA expression, Alizarin Red S staining Runx2 expression; whereas maintaining these an adipogenic culture medium gave time-dependent PPARγ FABP4 glycerol-3-phosphate dehydrogenase (GPDH) positivity...
Non-resolving lung inflammation and P. aeruginosa infections are the underlying cause of morbidity mortality in cystic fibrosis (CF). The endogenous lipid mediator resolvin (Rv) D1 is a potent regulator resolution its roles, actions, therapeutic potential CF interest. Here we investigated actions efficacy RvD1 preclinical models fibrosis. Cftr knockout mice with chronic infection were treated to assess differences bacterial load, inflammation, tissue damage. Cells from volunteers during ex...
An excessive, non-resolving inflammatory response underlies severe COVID-19 that may have fatal outcomes. Therefore, the investigation of endogenous pathways leading to resolution inflammation is interest uncover strategies for mitigating in people with SARS-CoV-2 infection. This becomes particularly urgent individuals preexisting pathologies characterized by chronic respiratory and prone bacterial infection, such as cystic fibrosis (CF). Here, we analyzed immune responses virion spike 1...
Abstract Background Innovative therapies to target tumor-associated neutrophils (PMN) are of clinical interest, since these cells centrally involved in cancer inflammation and tumor progression. Resolvin D1 (RvD1) is a lipid autacoid that promotes resolution by regulating the activity distinct immune non-immune cells. Here, using human papilloma virus (HPV) tumorigenesis as model, we investigated whether RvD1 modulates PMN reduce Methods Growth-curve assays with multiple cell lines vivo...
In addition to inhibiting cyclooxygenase (COX)-1-derived prostanoid biosynthesis, aspirin acetylates COX-2, enabling the conversion of arachidonic acid 15( R )-epi lipoxin A 4 , or aspirin-triggered (ATL). Selective COX-2 inhibitors block ATL formation and exacerbate mucosal injury in rats treated with aspirin. present study, we have examined whether inhibition activity healthy volunteers taking exacerbates gastric if such an effect would be prevented by NCX-4016, a NO-releasing derivative...
Insulin resistance is associated with a low chronic inflammatory state. In this study we investigated the relationship between impaired insulin sensitivity and selected markers of inflammation thrombin generation in obese healthy women. We examined 32 women (body mass index > or = 28), normal (NIS, n 14) (n 18), 10 nonobese < 25). Impaired patients had significantly higher levels C-reactive protein (CRP), TGF-beta 1, plasminogen activator inhibitor-1 (PAI-1), activated factor VII (VIIa),...
Impaired insulin-mediated vasodilation might contribute to vascular damage in insulin-resistant states. Little is known about insulin regulation of nitric oxide (NO) synthesis cells. The aim this work was investigate NO human umbilical vein endothelial cells (HUVECs) carrying the IRS-1 gene G972R variant, be associated with impaired activation PI3-kinase (PI3-K) pathway transfected cells.HUVECs were screened for presence G972R-IRS-1 (HUVEC-G972R) variant by restriction fragment length...
Background The peptido-leukotrienes (LTs) and lipoxins (LX) are produced by platelets through the transcellular conversion of leukocyte-derived LTA 4 at sites vascular inflammation injury, such as during coronary artery balloon angioplasty. We studied actions these eicosanoids on endothelium. Methods Results found that stimulation cultured human umbilical vein endothelial cells (EC) with LTC LTD resulted in release high-molecular-weight multimers von Willebrand factor (vWF) a concentration-...
Inflammatory lung disease is a primary cause of morbidity and mortality in cystic fibrosis (CF). Mechanisms unresolved acute inflammation CF are not completely known, although the involvement transmembrane conductance regulator (CFTR) nonrespiratory cells emerging. Here we examined CFTR expression function human platelets (PLTs) found that they express biologically active CFTR. blockade gave an ∼50% reduction lipoxin A4 (LXA4) formation during PLT/polymorphonuclear leukocytes (PMN)...